################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI HindIII HpaI MamI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1496 GGTACC G^GTACC AccIII 714 TCCGGA T^CCGGA Asp718I 1496 GGTACC G^GTACC AspI 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 158 TGCGCA TGC^GCA BalI 1614 TGGCCA TGG^CCA BbrPI 1240 CACGTG CAC^GTG BseAI 714 TCCGGA T^CCGGA BsgI 1237 CTGCAC GTGCAG(16/14) BsmFI 213 GTCCC GGGAC(10/14) BspEI 714 TCCGGA T^CCGGA BssSI 1636 CTCGTG CACGAG(-5/-1) BstAPI 1626 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1286 AGATCC [GA]^GATC[CT] DraIII 205 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1552 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 257 CGGCCG C^GGCCG EclXI 257 CGGCCG C^GGCCG Eco52I 257 CGGCCG C^GGCCG EcoNI 1048 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 379 GGCCGGCC GGCCGG^CC FspI 158 TGCGCA TGC^GCA HgaI 560 GCGTC GACGC(5/10) HincII 1273 GTCAAC GT[CT]^[GA]AC HindII 1273 GTCAAC GT[CT]^[GA]AC KpnI 1496 GGTACC GGTAC^C KspI 243 CCGCGG CCGC^GG MflI 1286 AGATCC [GA]^GATC[CT] MluNI 1614 TGGCCA TGG^CCA MroI 714 TCCGGA T^CCGGA MscI 1614 TGGCCA TGG^CCA NcoI 982 CCATGG C^CATGG NdeI 803 CATATG CA^TATG PflFI 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1240 CACGTG CAC^GTG PmlI 1240 CACGTG CAC^GTG PshAI 45 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 294 CAGCTG CAG^CTG SacI 904 GAGCTC GAGCT^C SacII 243 CCGCGG CCGC^GG SapI 1566 GAAGAGC GCTCTTC(1/4) ScaI 473 AGTACT AGT^ACT SexAI 437 ACCAGGT A^CC[AT]GGT SfiI 238 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1378 CTCAAG C^T[CT][GA]AG SphI 1604 GCATGC GCATG^C SspI 920 AATATT AAT^ATT StuI 1298 AGGCCT AGG^CCT Tsp509I 537 AATT ^AATT Tth111I 1669 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1291 CCACCACAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1286 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 1287 GATCC GGATC(4/5) 1656 GATCC AlwNI 439 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 967 CAGAGTCTG AspHI 904 GAGCTC G[AT]GC[AT]^C 1569 GAGCAC BbsI 1145 GAAGAC GAAGAC(2/6) 1171 GAAGAC BpuAI 1145 GAAGAC GAAGAC(2/6) 1171 GAAGAC BsaAI 463 TACGTG [CT]AC^GT[GA] 1240 CACGTG BsaI 776 GAGACC GGTCTC(1/5) 509 GGTCTC BsaWI 714 TCCGGA [AT]^CCGG[AT] 908 TCCGGT BseRI 1049 CTCCTC GAGGAG(10/8) 994 GAGGAG BsiEI 50 CGGTCG CG[GA][CT]^CG 257 CGGCCG BsiHKAI 904 GAGCTC G[AT]GC[AT]^C 1569 GAGCAC BsmI 850 GAATGC GAATGC(1/-1) 962 GAATGC BsrFI 380 GCCGGC [GA]^CCGG[CT] 1305 GCCGGC Cfr10I 380 GCCGGC [GA]^CCGG[CT] 1305 GCCGGC MslI 459 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1599 CACGGGCATG NaeI 380 GCCGGC GCC^GGC 1305 GCCGGC NgoMIV 380 GCCGGC G^CCGGC 1305 GCCGGC NspI 746 ACATGC [GA]CATG^[CT] 1604 GCATGC PflMI 438 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1386 CCAGCCCTTGG PpuMI 815 AGGACCC [GA]G^G[AT]CC[CT] 1201 GGGTCCT PstI 297 CTGCAG CTGCA^G 1736 CTGCAG SmaI 263 CCCGGG CCC^GGG 1581 CCCGGG TfiI 738 GAATC G^A[AT]TC 942 GATTC Van91I 438 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1386 CCAGCCCTTGG XmaCI 263 CCCGGG C^CCGGG 1581 CCCGGG XmaI 263 CCCGGG C^CCGGG 1581 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrBI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I