(Circular) (Six-base) MAPSORT of: pFN21AE1326.seq Check: 8554 from: 1 to: 6427 pFN21AE1326 6427 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2811 4237 278 Size: 53 83 186 936 1275 1426 2468 Fragments arranged by size: 2468 1426 1275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3462 3748 3462 Size: 286 6141 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4490 5874 6142 1632 Size: 2858 1384 268 1917 Fragments arranged by size: 2858 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 5326 6303 1203 Size: 4123 977 1327 Fragments arranged by size: 4123 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3291 1184 Size: 2107 4320 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2012 2044 2558 2590 2012 Size: 32 514 32 5849 Fragments arranged by size: 5849 514 32 32 AlwNI CAG_nnn'CTG Cuts at: 2155 2185 2554 3124 3454 4170 5742 2155 Size: 30 369 570 330 716 1572 2840 Fragments arranged by size: 2840 1572 716 570 369 330 30 ApaI G_GGCC'C Cuts at: 2776 2776 Size: 6427 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 3736 3962 4114 6223 1493 Size: 2243 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2789 3279 3471 3752 1870 Size: 73 846 490 192 281 4545 Fragments arranged by size: 4545 846 490 281 192 73 AvrII C'CTAG_G Cuts at: 3022 3022 Size: 6427 BamHI G'GATC_C Cuts at: 2499 3757 4124 2499 Size: 1258 367 4802 Fragments arranged by size: 4802 1258 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3462 3748 5203 618 Size: 325 217 627 1675 286 1455 1842 Fragments arranged by size: 1842 1675 1455 627 325 286 217 BanII G_rGCy'C Cuts at: 1391 2098 2250 2546 2673 2776 3746 1391 Size: 707 152 296 127 103 970 4072 Fragments arranged by size: 4072 970 707 296 152 127 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3532 928 Size: 971 1633 3823 Fragments arranged by size: 3823 1633 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2366 2400 2870 2904 4618 4652 1903 Size: 34 429 34 470 34 1714 34 3678 Fragments arranged by size: 3678 1714 470 429 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 2325 1548 Size: 777 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 2754 2897 Size: 107 122 71 631 605 463 619 143 Cuts at: 2897 3284 3578 5038 136 Size: 387 294 1460 1525 Fragments arranged by size: 1525 1460 631 619 605 463 387 294 143 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3442 3716 3844 3442 Size: 274 128 6025 Fragments arranged by size: 6025 274 128 Bme1580I G_kGCm'C Cuts at: 1790 2162 2776 4488 5644 1790 Size: 372 614 1712 1156 2573 Fragments arranged by size: 2573 1712 1156 614 372 BmgBI CAC'GTC Cuts at: 1318 2884 1318 Size: 1566 4861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2505 4158 5116 Size: 416 53 83 399 24 1088 1653 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 1753 1653 1088 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2082 3005 3146 3549 5087 1239 Size: 843 923 141 403 1538 2579 Fragments arranged by size: 2579 1538 923 843 403 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 4552 5417 5715 2300 Size: 2252 865 298 3012 Fragments arranged by size: 3012 2252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2056 2808 3645 5090 882 Size: 617 557 752 837 1445 2219 Fragments arranged by size: 2219 1445 837 752 617 557 BsaAI yAC'GTr Cuts at: 493 1204 4218 493 Size: 711 3014 2702 Fragments arranged by size: 3014 2702 711 BsaBI GATnn'nnATC Cuts at: 3591 6421 3591 Size: 2830 3597 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2736 2808 4234 4616 Size: 53 83 186 936 1203 72 1426 382 Cuts at: 4616 5251 275 Size: 635 1451 Fragments arranged by size: 1451 1426 1203 936 635 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 2482 2577 2911 4860 5258 5532 5679 6090 2482 Size: 95 334 1949 398 274 147 411 2819 Fragments arranged by size: 2819 1949 411 398 334 274 147 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 3642 3672 1178 Size: 30 165 30 822 30 1387 30 3933 Fragments arranged by size: 3933 1387 822 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3335 3549 1186 Size: 2149 214 4064 Fragments arranged by size: 4064 2149 214 BseYI C'CCAG_C Cuts at: 2155 3037 3447 5630 6126 2155 Size: 882 410 2183 496 2456 Fragments arranged by size: 2456 2183 882 496 410 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5854 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4488 4573 5644 4488 Size: 85 1071 5271 Fragments arranged by size: 5271 1071 85 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2162 2250 2546 2673 2776 3746 Size: 399 308 64 88 296 127 103 970 Cuts at: 3746 4488 4573 5644 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 970 742 399 308 296 127 103 88 85 64 BspEI T'CCGG_A Cuts at: 2577 2911 6090 2577 Size: 334 3179 2914 Fragments arranged by size: 3179 2914 334 BspHI T'CATG_A Cuts at: 3705 4316 6046 3705 Size: 611 1730 4086 Fragments arranged by size: 4086 1730 611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2420 2826 3782 844 Size: 1576 406 956 3489 Fragments arranged by size: 3489 1576 956 406 BsrBI CCG'CTC Cuts at: 1394 1823 3441 3498 4314 1394 Size: 429 1618 57 816 3507 Fragments arranged by size: 3507 1618 816 429 57 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 2343 3483 4922 5096 65 Size: 1183 1026 69 1140 1439 174 1396 Fragments arranged by size: 1439 1396 1183 1140 1026 174 69 BsrFI r'CCGG_y Cuts at: 1700 1810 2448 2638 3306 5071 5258 1700 Size: 110 638 190 668 1765 187 2869 Fragments arranged by size: 2869 1765 668 638 190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 2375 4487 5499 2375 Size: 2112 1012 3303 Fragments arranged by size: 3303 2112 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 3485 794 Size: 1391 1300 3736 Fragments arranged by size: 3736 1391 1300 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 2713 3364 1184 Size: 1529 651 4247 Fragments arranged by size: 4247 1529 651 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2884 2467 Size: 417 6010 BstYI r'GATC_y Cuts at: 1972 2487 2499 3033 3757 4124 4509 4526 5978 Size: 515 12 534 724 367 385 17 1452 Cuts at: 5978 6064 6076 6422 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 724 534 515 385 367 346 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2250 2304 2365 2454 3631 513 Size: 552 1185 54 61 89 1177 3309 Fragments arranged by size: 3309 1185 1177 552 89 61 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2380 2446 3186 487 Size: 683 56 1154 66 740 3728 Fragments arranged by size: 3728 1154 740 683 66 56 BtsI GCAGTG_nn' Cuts at: 2160 3303 3943 4737 4757 2160 Size: 1143 640 794 20 3830 Fragments arranged by size: 3830 1143 794 640 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DraIII CAC_nnn'GTG Cuts at: 2604 2604 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2457 3149 3436 Size: 54 1425 50 275 18 627 692 287 Cuts at: 3436 3874 4763 6392 8 Size: 438 889 1629 43 Fragments arranged by size: 1629 1425 889 692 627 438 287 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2960 4357 1353 Size: 1607 1397 3423 Fragments arranged by size: 3423 1607 1397 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 3005 3146 3549 4490 5087 5874 Size: 393 450 923 141 403 941 597 787 Cuts at: 5874 6142 1239 Size: 268 1524 Fragments arranged by size: 1524 941 923 787 597 450 403 393 268 141 EcoO109I rG'GnC_Cy Cuts at: 2772 3019 2772 Size: 247 6180 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3256 3256 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2314 3082 3341 5574 1678 Size: 636 768 259 2233 2531 Fragments arranged by size: 2531 2233 768 636 259 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2080 2112 2225 Size: 32 135 32 437 32 39 32 113 Cuts at: 2225 2257 2472 2504 2910 2942 5068 5100 5142 Size: 32 215 32 406 32 2126 32 42 Cuts at: 5142 5174 1373 Size: 32 2626 Fragments arranged by size: 2626 2126 437 406 215 135 113 42 39 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6427 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3466 3752 3466 Size: 286 6141 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 3628 5541 5725 1305 Size: 1197 1126 1913 184 2007 Fragments arranged by size: 2007 1913 1197 1126 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 10 Size: 54 1425 343 627 3978 Fragments arranged by size: 3978 1425 627 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3005 3119 3419 3710 4385 4744 4903 Size: 648 1839 114 300 291 675 359 159 Cuts at: 4903 518 Size: 2042 Fragments arranged by size: 2042 1839 675 648 359 300 291 159 114 MspA1I CmG'CkG Cuts at: 1606 2111 2185 2192 3313 3843 4520 5668 5913 Size: 505 74 7 1121 530 677 1148 245 Cuts at: 5913 1606 Size: 2120 Fragments arranged by size: 2120 1148 1121 677 530 505 245 74 7 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5875 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 3272 3785 5330 1132 Size: 97 2043 513 1545 2229 Fragments arranged by size: 2229 2043 1545 513 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6427 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 2554 1108 Size: 1047 399 4981 Fragments arranged by size: 4981 1047 399 PfoI T'CCnGG_A Cuts at: 2013 2098 2211 2406 2982 2013 Size: 85 113 195 576 5458 Fragments arranged by size: 5458 576 195 113 85 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3019 3019 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspOMI G'GGCC_C Cuts at: 2772 2772 Size: 6427 PstI C_TGCA'G Cuts at: 830 2037 2508 3779 830 Size: 1207 471 1271 3478 Fragments arranged by size: 3478 1271 1207 471 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2185 2192 3313 2185 Size: 7 1121 5299 Fragments arranged by size: 5299 1121 7 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SbfI CC_TGCA'GG Cuts at: 2508 3779 2508 Size: 1271 5156 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SexAI A'CCwGG_T Cuts at: 3654 3654 Size: 6427 SfcI C'TryA_G Cuts at: 826 1045 2033 2504 3775 4910 5591 5782 826 Size: 219 988 471 1271 1135 681 191 1471 Fragments arranged by size: 1471 1271 1135 988 681 471 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2135 2135 Size: 6427 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 2791 3281 3754 1872 Size: 919 490 473 4545 Fragments arranged by size: 4545 919 490 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 4531 5432 5694 820 Size: 197 926 372 2216 901 262 1553 Fragments arranged by size: 2216 1553 926 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3272 3785 1132 Size: 2140 513 3774 Fragments arranged by size: 3774 2140 513 SrfI GCCC'GGGC Cuts at: 2791 2791 Size: 6427 SspI AAT'ATT Cuts at: 5 52 2623 4351 5 Size: 47 2571 1728 2081 Fragments arranged by size: 2571 2081 1728 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6427 StyI C'CwwG_G Cuts at: 513 1065 2475 3022 3231 3866 513 Size: 552 1410 547 209 635 3074 Fragments arranged by size: 3074 1410 635 552 547 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2031 2596 4454 4471 4624 4809 5254 Size: 1255 451 565 1858 17 153 185 445 Cuts at: 5254 325 Size: 1498 Fragments arranged by size: 1858 1498 1255 565 451 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4673 6385 96 Size: 274 80 33 51 1443 2696 1712 138 Fragments arranged by size: 2696 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3642 3674 6387 6419 3642 Size: 32 2713 32 3650 Fragments arranged by size: 3650 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2849 1316 Size: 1533 4894 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3121 3187 3379 3573 3121 Size: 66 192 194 5975 Fragments arranged by size: 5975 194 192 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 2789 3279 3752 1870 Size: 919 490 473 4545 Fragments arranged by size: 4545 919 490 473 XmnI GAAnn'nnTTC Cuts at: 3050 4556 3050 Size: 1506 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2809 4235 276 Size: 53 83 186 936 1275 1426 2468 Fragments arranged by size: 2468 1426 1275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SwaI