################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI SwaI TaqI Tru9I TseI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AlwNI 475 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 316 CCTCAGG CC^T[ACGT]AGG AspHI 220 GAGCTC G[AT]GC[AT]^C AvaII 276 GGACC G^G[AT]CC BamHI 81 GGATCC G^GATCC BanII 220 GAGCTC G[GA]GC[CT]^C BcnI 273 CCGGG CC^[GC]GG BfaI 229 CTAG C^TAG BglI 395 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 430 GGTCTC GGTCTC(1/5) BseRI 241 GAGGAG GAGGAG(10/8) BsiHKAI 220 GAGCTC G[AT]GC[AT]^C BsmBI 264 GAGACG CGTCTC(1/5) BsmFI 275 GGGAC GGGAC(10/14) Bsp1407I 329 TGTACA T^GTACA BspMI 98 ACCTGC ACCTGC(4/8) BsrGI 329 TGTACA T^GTACA BstAPI 474 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 426 CCAGG CC^[AT]GG BstYI 81 GGATCC [GA]^GATC[CT] Bsu36I 316 CCTCAGG CC^T[ACGT]AGG DpnII 82 GATC ^GATC DsaI 23 CCGCGG C^C[GA][CT]GG Eco81I 316 CCTCAGG CC^T[ACGT]AGG EcoRII 426 CCAGG ^CC[AT]GG EcoT14I 446 CCAAGG C^C[AT][AT]GG FauI 184 CCCGC CCCGC(4/6) HinfI 110 GAATC G^A[ACGT]TC HphI 20 TCACC GGTGA(8/7) KspI 23 CCGCGG CCGC^GG MaeI 229 CTAG C^TAG MaeII 267 ACGT A^CGT MboI 82 GATC ^GATC MflI 81 GGATCC [GA]^GATC[CT] MspA1I 23 CCGCGG C[AC]G^C[GT]G MvaI 426 CCAGG CC^[AT]GG NciI 273 CCGGG CC^[GC]GG NdeII 82 GATC ^GATC NlaIII 471 CATG CATG^ NspI 470 ACATGC [GA]CATG^[CT] PpuMI 275 GGGACCC [GA]G^G[AT]CC[CT] SacI 220 GAGCTC GAGCT^C SacII 23 CCGCGG CCGC^GG Sau3AI 82 GATC ^GATC SbfI 33 CCTGCAGG CCTGCA^GG Sse8387I 33 CCTGCAGG CCTGCA^GG SspBI 329 TGTACA T^GTACA StyI 446 CCAAGG C^C[AT][AT]GG TfiI 110 GAATC G^A[AT]TC XhoII 81 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 24 CGCG CG^CG 186 CGCG AcyI 178 GACGCC G[GA]^CG[CT]C 460 GACGCC AlwI 82 GATCC GGATC(4/5) 81 GGATC BmyI 220 GAGCTC G[AGT]GC[ACT]^C 409 GGGCAC BsaHI 178 GACGCC G[GA]^CG[CT]C 460 GACGCC BsmAI 264 GAGAC GTCTC(1/5) 431 GTCTC Bsp1286I 220 GAGCTC G[AGT]GC[ACT]^C 409 GGGCAC BssKI 273 CCGGG ^CC[ACGT]GG 426 CCAGG BstUI 24 CGCG CG^CG 186 CGCG DraII 275 GGGACCC [GA]G^G[ACGT]CC[CT] 476 AGGCCCT EaeI 26 CGGCCA [CT]^GGCC[GA] 270 TGGCCG EarI 115 CTCTTC CTCTTC(1/4) 236 GAAGAG EcoNI 316 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 420 CCTTTACCAGG EcoO109I 275 GGGACCC [GA]G^G[ACGT]CC[CT] 476 AGGCCCT Fnu4HI 25 GCGGC GC^[ACGT]GC 297 GCCGC HaeII 12 GGCGCT [GA]GCGC^[CT] 392 AGCGCC HapII 273 CCGG C^CGG 396 CCGG HgaI 178 GACGC GACGC(5/10) 460 GACGC Hin1I 178 GACGCC G[GA]^CG[CT]C 460 GACGCC HpaII 273 CCGG C^CGG 396 CCGG ItaI 25 GCGGC GC^[ACGT]GC 297 GCCGC Ksp632I 115 CTCTTC CTCTTC(1/4) 236 GAAGAG MspI 273 CCGG C^CGG 396 CCGG MvnI 24 CGCG CG^CG 186 CGCG MwoI 395 GCCGGAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 474 GCAGGCCCTGC PstI 34 CTGCAG CTGCA^G 340 CTGCAG ScrFI 273 CCGGG CC^[ACGT]GG 426 CCAGG StuI 324 AGGCCT AGG^CCT 362 AGGCCT TspRI 138 CACTG CA[GC]TG[ACGT][ACGT]^ 369 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AfaI AluI BsaJI BsiYI BslI BstF5I Cac8I CfoI Cfr13I DdeI FokI HaeIII HhaI HinP1I MboII MnlI NlaIV RsaI Sau96I SfcI