(Linear) (Six-base) MAPSORT of: KIBB6231.seq Check: 5426 from: 1 to: 2229 KIBB6231.orf With 182 enzymes: SgfI * March 22, 2012 15:33 .. AflIII A'CryG_T Cuts at: 0 1814 2229 Size: 1814 415 AgeI A'CCGG_T Cuts at: 0 1651 2229 Size: 1651 578 AhdI GACnn_n'nnGTC Cuts at: 0 1290 2229 Size: 1290 939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1978 2229 Size: 1978 251 AlwNI CAG_nnn'CTG Cuts at: 0 1707 1773 2229 Size: 1707 66 456 Fragments arranged by size: 1707 456 66 ApaI G_GGCC'C Cuts at: 0 248 1489 2006 2229 Size: 248 1241 517 223 Fragments arranged by size: 1241 517 248 223 ApaLI G'TGCA_C Cuts at: 0 313 1867 2229 Size: 313 1554 362 Fragments arranged by size: 1554 362 313 ApoI r'AATT_y Cuts at: 0 490 829 2229 Size: 490 339 1400 Fragments arranged by size: 1400 490 339 AvaI C'yCGr_G Cuts at: 0 1159 1265 1419 1512 1659 2061 2229 Size: 1159 106 154 93 147 402 168 Fragments arranged by size: 1159 402 168 154 147 106 93 BanI G'GyrC_C Cuts at: 0 72 172 358 1544 1585 1938 2229 Size: 72 100 186 1186 41 353 291 Fragments arranged by size: 1186 353 291 186 100 72 41 BanII G_rGCy'C Cuts at: 0 248 284 1208 1369 1489 2006 2068 2229 Size: 248 36 924 161 120 517 62 161 Fragments arranged by size: 924 517 248 161 161 120 62 36 BbeI G_GCGC'C Cuts at: 0 76 176 1589 2229 Size: 76 100 1413 640 Fragments arranged by size: 1413 640 100 76 BbsI GAAGACnn'nnnn_ Cuts at: 0 277 2229 Size: 277 1952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2042 2076 2229 Size: 2042 34 153 Fragments arranged by size: 2042 153 34 BclI T'GATC_A Cuts at: 0 675 2229 Size: 675 1554 BglI GCCn_nnn'nGGC Cuts at: 0 1523 1696 1831 2029 2041 2050 2229 Size: 1523 173 135 198 12 9 179 Fragments arranged by size: 1523 198 179 173 135 12 9 BglII A'GATC_T Cuts at: 0 1181 2229 Size: 1181 1048 BlpI GC'TnA_GC Cuts at: 0 1256 2229 Size: 1256 973 Bme1580I G_kGCm'C Cuts at: 0 248 317 361 1384 1489 1547 1871 2006 Size: 248 69 44 1023 105 58 324 135 Cuts at: 2006 2229 Size: 223 Fragments arranged by size: 1023 324 248 223 135 105 69 58 44 BmgBI CAC'GTC Cuts at: 0 1196 1755 2229 Size: 1196 559 474 Fragments arranged by size: 1196 559 474 BmrI ACTGGGnnnn_n' Cuts at: 0 587 864 2229 Size: 587 277 1365 Fragments arranged by size: 1365 587 277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1002 1046 1578 1729 1842 2085 2229 Size: 340 662 44 532 151 113 243 144 Fragments arranged by size: 662 532 340 243 151 144 113 44 BsaI GGTCTCn'nnnn_ Cuts at: 0 211 2229 Size: 211 2018 BsaAI yAC'GTr Cuts at: 0 686 1817 2229 Size: 686 1131 412 Fragments arranged by size: 1131 686 412 BsaHI Gr'CG_yC Cuts at: 0 73 173 1127 1586 1717 2229 Size: 73 100 954 459 131 512 Fragments arranged by size: 954 512 459 131 100 73 BsaWI w'CCGG_w Cuts at: 0 21 1651 2229 Size: 21 1630 578 Fragments arranged by size: 1630 578 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 123 153 2068 2098 2229 Size: 123 30 1915 30 131 Fragments arranged by size: 1915 131 123 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 225 1162 1216 1369 1833 2076 2088 2229 Size: 225 937 54 153 464 243 12 141 Fragments arranged by size: 937 464 243 225 153 141 54 12 BseYI C'CCAG_C Cuts at: 0 99 1108 1564 2229 Size: 99 1009 456 665 Fragments arranged by size: 1009 665 456 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1471 1543 2229 Size: 1471 72 686 Fragments arranged by size: 1471 686 72 BsiEI CG_ry'CG Cuts at: 0 215 1466 1607 1838 1997 2229 Size: 215 1251 141 231 159 232 Fragments arranged by size: 1251 232 231 215 159 141 BsiHKAI G_wGCw'C Cuts at: 0 317 1871 2229 Size: 317 1554 358 Fragments arranged by size: 1554 358 317 Bsp1286I G_dGCh'C Cuts at: 0 248 284 317 361 1208 1369 1384 1489 Size: 248 36 33 44 847 161 15 105 Cuts at: 1489 1547 1871 2006 2068 2229 Size: 58 324 135 62 161 Fragments arranged by size: 847 324 248 161 161 135 105 62 58 44 36 33 15 BsrBI CCG'CTC Cuts at: 0 1260 1443 1551 2084 2229 Size: 1260 183 108 533 145 Fragments arranged by size: 1260 533 183 145 108 BsrFI r'CCGG_y Cuts at: 0 220 1389 1651 1941 2229 Size: 220 1169 262 290 288 Fragments arranged by size: 1169 290 288 262 220 BsrGI T'GTAC_A Cuts at: 0 551 2229 Size: 551 1678 BssHII G'CGCG_C Cuts at: 0 1408 1410 1412 2229 Size: 1408 2 2 817 Fragments arranged by size: 1408 817 2 2 BstEII G'GTnAC_C Cuts at: 0 103 2229 Size: 103 2126 BstXI CCAn_nnnn'nTGG Cuts at: 0 1680 2229 Size: 1680 549 BstYI r'GATC_y Cuts at: 0 18 1181 2229 Size: 18 1163 1048 Fragments arranged by size: 1163 1048 18 BtgI C'CryG_G Cuts at: 0 275 1229 1577 1631 1673 1679 1721 1906 Size: 275 954 348 54 42 6 42 185 Cuts at: 1906 1985 1997 2229 Size: 79 12 232 Fragments arranged by size: 954 348 275 232 185 79 54 42 42 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 819 2229 Size: 819 1410 BtsI GCAGTG_nn' Cuts at: 0 2060 2229 Size: 2060 169 DraI TTT'AAA Cuts at: 0 408 2229 Size: 408 1821 DrdI GACnn_nn'nnGTC Cuts at: 0 2017 2229 Size: 2017 212 EaeI y'GGCC_r Cuts at: 0 179 212 924 1175 1391 1463 1604 1628 Size: 179 33 712 251 216 72 141 24 Cuts at: 1628 1676 1724 1775 1823 1835 1994 2030 2147 Size: 48 48 51 48 12 159 36 117 Cuts at: 2147 2229 Size: 82 Fragments arranged by size: 712 251 216 179 159 141 117 82 72 51 48 48 48 36 33 24 12 EagI C'GGCC_G Cuts at: 0 212 1463 1604 1835 1994 2229 Size: 212 1251 141 231 159 235 Fragments arranged by size: 1251 235 231 212 159 141 EarI CTCTTCn'nnn_ Cuts at: 0 284 924 1397 1558 2229 Size: 284 640 473 161 671 Fragments arranged by size: 671 640 473 284 161 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1002 1046 1578 1729 1842 2085 2229 Size: 340 662 44 532 151 113 243 144 Fragments arranged by size: 662 532 340 243 151 144 113 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 1480 2229 Size: 1480 749 EcoO109I rG'GnC_Cy Cuts at: 0 244 245 1001 1485 1486 1618 1643 1770 Size: 244 1 756 484 1 132 25 127 Cuts at: 1770 2002 2003 2210 2229 Size: 232 1 207 19 Fragments arranged by size: 756 484 244 232 207 132 127 25 19 1 1 1 EcoRI G'AATT_C Cuts at: 0 829 2229 Size: 829 1400 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1449 2229 Size: 1449 780 HaeII r_GCGC'y Cuts at: 0 76 160 176 1390 1589 2229 Size: 76 84 16 1214 199 640 Fragments arranged by size: 1214 640 199 84 76 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 8 40 123 155 180 212 1000 1032 Size: 8 32 83 32 25 32 788 32 Cuts at: 1032 1276 1308 2050 2082 2229 Size: 244 32 742 32 147 Fragments arranged by size: 788 742 244 147 83 32 32 32 32 32 32 25 8 KasI G'GCGC_C Cuts at: 0 72 172 1585 2229 Size: 72 100 1413 644 Fragments arranged by size: 1413 644 100 72 MfeI C'AATT_G Cuts at: 0 199 2229 Size: 199 2030 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1178 2229 Size: 1178 1051 MscI TGG'CCA Cuts at: 0 181 1630 1777 2229 Size: 181 1449 147 452 Fragments arranged by size: 1449 452 181 147 MslI CAynn'nnrTG Cuts at: 0 364 838 967 1678 1819 1936 2229 Size: 364 474 129 711 141 117 293 Fragments arranged by size: 711 474 364 293 141 129 117 MspA1I CmG'CkG Cuts at: 0 1231 1274 1609 1693 1723 1831 1908 1999 Size: 1231 43 335 84 30 108 77 91 Cuts at: 1999 2175 2229 Size: 176 54 Fragments arranged by size: 1231 335 176 108 91 84 77 54 43 30 NaeI GCC'GGC Cuts at: 0 1391 2229 Size: 1391 838 NarI GG'CG_CC Cuts at: 0 73 173 1586 2229 Size: 73 100 1413 643 Fragments arranged by size: 1413 643 100 73 NcoI C'CATG_G Cuts at: 0 275 1577 1679 1985 2229 Size: 275 1302 102 306 244 Fragments arranged by size: 1302 306 275 244 102 NgoMIV G'CCGG_C Cuts at: 0 1389 2229 Size: 1389 840 NotI GC'GGCC_GC Cuts at: 0 1463 1604 1835 1994 2229 Size: 1463 141 231 159 235 Fragments arranged by size: 1463 235 231 159 141 NruI TCG'CGA Cuts at: 0 1376 2229 Size: 1376 853 PasI CC'CwG_GG Cuts at: 0 240 1482 2229 Size: 240 1242 747 Fragments arranged by size: 1242 747 240 PflMI CCAn_nnn'nTGG Cuts at: 0 2178 2229 Size: 2178 51 PfoI T'CCnGG_A Cuts at: 0 908 1946 2229 Size: 908 1038 283 Fragments arranged by size: 1038 908 283 PpuMI rG'GwC_Cy Cuts at: 0 1001 1618 2210 2229 Size: 1001 617 592 19 Fragments arranged by size: 1001 617 592 19 PsiI TTA'TAA Cuts at: 0 488 2229 Size: 488 1741 PspOMI G'GGCC_C Cuts at: 0 244 1485 2002 2229 Size: 244 1241 517 227 Fragments arranged by size: 1241 517 244 227 PstI C_TGCA'G Cuts at: 0 959 2165 2229 Size: 959 1206 64 Fragments arranged by size: 1206 959 64 SacII CC_GC'GG Cuts at: 0 1232 1724 1909 2000 2229 Size: 1232 492 185 91 229 Fragments arranged by size: 1232 492 229 185 91 SanDI GG'GwC_CC Cuts at: 0 1618 2229 Size: 1618 611 SapI GCTCTTCn'nnn_ Cuts at: 0 1558 2229 Size: 1558 671 SfcI C'TryA_G Cuts at: 0 955 1076 1917 2161 2229 Size: 955 121 841 244 68 Fragments arranged by size: 955 841 244 121 68 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1523 2229 Size: 1523 706 SfoI GGC'GCC Cuts at: 0 74 174 1587 2229 Size: 74 100 1413 642 Fragments arranged by size: 1413 642 100 74 SgrAI Cr'CCGG_yG Cuts at: 0 1651 2229 Size: 1651 578 SmaI CCC'GGG Cuts at: 0 1267 1421 1514 2229 Size: 1267 154 93 715 Fragments arranged by size: 1267 715 154 93 StuI AGG'CCT Cuts at: 0 264 1165 1432 2229 Size: 264 901 267 797 Fragments arranged by size: 901 797 267 264 StyI C'CwwG_G Cuts at: 0 275 308 1394 1577 1679 1985 2229 Size: 275 33 1086 183 102 306 244 Fragments arranged by size: 1086 306 275 244 183 102 33 TatI w'GTAC_w Cuts at: 0 551 796 2229 Size: 551 245 1433 Fragments arranged by size: 1433 551 245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 189 315 2229 Size: 189 126 1914 Fragments arranged by size: 1914 189 126 XmaI C'CCGG_G Cuts at: 0 1265 1419 1512 2229 Size: 1265 154 93 717 Fragments arranged by size: 1265 717 154 93 Enzymes that do cut: AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssHII BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI PasI PflMI PfoI PpuMI PsiI PspOMI PstI SacII SanDI SapI SfcI SfiI SfoI SgrAI SmaI StuI StyI TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AleI AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BmtI BplI Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraIII EciI EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI NdeI NheI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI