################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI Fnu4HI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI ItaI KasI KspI MaeI MaeII MaeIII MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI TseI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 78 GGTACC G^GTACC AciI 160 CCGC CCGC(-3/-1) AfaI 79 GTAC GT^AC AluI 290 AGCT AG^CT Aor51HI 308 AGCGCT AGC^GCT Asp718I 78 GGTACC G^GTACC AspHI 202 GAGCAC G[AT]GC[AT]^C BalI 320 TGGCCA TGG^CCA BanI 78 GGTACC G^G[CT][GA]CC BbsI 112 GAAGAC GAAGAC(2/6) BmyI 202 GAGCAC G[AGT]GC[ACT]^C BpuAI 112 GAAGAC GAAGAC(2/6) BsaBI 210 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 304 GAGGAG GAGGAG(10/8) BsgI 223 GTGCAG GTGCAG(16/14) BsiHKAI 202 GAGCAC G[AT]GC[AT]^C Bsp1286I 202 GAGCAC G[AGT]GC[ACT]^C BsrI 123 ACTGG ACTGG(1/-1) BssKI 249 CCAGG ^CC[ACGT]GG BstF5I 216 CATCC GGATG(2/0) BstNI 249 CCAGG CC^[AT]GG Cac8I 175 GCTGGC GC[ACGT]^[ACGT]GC CfoI 309 GCGC GCG^C EaeI 320 TGGCCA [CT]^GGCC[GA] EarI 34 CTCTTC CTCTTC(1/4) Eco47III 308 AGCGCT AGC^GCT EcoNI 297 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 249 CCAGG ^CC[AT]GG FauI 159 CCCGC CCCGC(4/6) FokI 216 CATCC GGATG(9/13) HaeII 308 AGCGCT [GA]GCGC^[CT] HaeIII 321 GGCC GG^CC HapII 235 CCGG C^CGG HhaI 309 GCGC GCG^C HinP1I 309 GCGC G^CGC HinfI 22 GAGTC G^A[ACGT]TC HpaII 235 CCGG C^CGG KpnI 78 GGTACC GGTAC^C Ksp632I 34 CTCTTC CTCTTC(1/4) MamI 210 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MslI 216 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 235 CCGG C^CGG MvaI 249 CCAGG CC^[AT]GG NlaIV 78 GGTACC GG[ACGT]^[ACGT]CC PleI 22 GAGTC GAGTC(4/5) RsaI 79 GTAC GT^AC SapI 33 GCTCTTC GCTCTTC(1/4) ScrFI 249 CCAGG CC^[ACGT]GG SfcI 240 CTACAG C^T[GA][CT]AG Tsp509I 345 AATT ^AATT ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BpmI 11 CTCCAG CTGGAG(16/14) 301 CTGGAG BsmFI 187 GTCCC GGGAC(10/14) 294 GTCCC DpnII 54 GATC ^GATC 210 GATC EcoT14I 74 CCAAGG C^C[AT][AT]GG 197 CCTTGG MboI 54 GATC ^GATC 210 GATC MseI 62 TTAA T^TAA 137 TTAA NdeII 54 GATC ^GATC 210 GATC PflMI 117 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 192 CCACCCCTTGG Sau3AI 54 GATC ^GATC 210 GATC StyI 74 CCAAGG C^C[AT][AT]GG 197 CCTTGG TaqI 20 TCGA T^CGA 38 TCGA Tru9I 62 TTAA T^TAA 137 TTAA TspRI 122 CACTG CA[GC]TG[ACGT][ACGT]^ 285 CAGTG TthHB8I 20 TCGA T^CGA 38 TCGA Van91I 117 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 192 CCACCCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BsaJI BsiYI BslI HphI MboII MnlI MwoI