(Circular) (Six-base) MAPSORT of: pFN21AE4540.seq Check: 4990 from: 1 to: 6571 pFN21AE4540 6571 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2676 2676 Size: 6571 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3313 3914 1937 Size: 1376 601 4594 Fragments arranged by size: 4594 1376 601 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 3006 3123 4634 6018 6286 1632 Size: 599 775 117 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 775 599 268 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6374 AflIII A'CryG_T Cuts at: 1203 5470 6447 1203 Size: 4267 977 1327 Fragments arranged by size: 4267 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 2139 1166 Size: 973 5598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3608 3640 3608 Size: 32 6539 AlwNI CAG_nnn'CTG Cuts at: 2865 3404 4314 5886 2865 Size: 539 910 1572 3550 Fragments arranged by size: 3550 1572 910 539 ApaLI G'TGCA_C Cuts at: 4628 5784 4628 Size: 1156 5415 ApoI r'AATT_y Cuts at: 1493 3575 3752 3880 4106 4258 6367 1493 Size: 2082 177 128 226 152 2109 1697 Fragments arranged by size: 2109 2082 1697 226 177 152 128 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2726 2906 3214 3896 1870 Size: 73 153 630 180 308 682 4545 Fragments arranged by size: 4545 682 630 308 180 153 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2595 3892 5347 618 Size: 325 217 627 808 1297 1455 1842 Fragments arranged by size: 1842 1455 1297 808 627 325 217 BanII G_rGCy'C Cuts at: 1391 2456 3182 3890 1391 Size: 1065 726 708 4072 Fragments arranged by size: 4072 1065 726 708 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2879 3035 3780 928 Size: 971 980 156 745 3719 Fragments arranged by size: 3719 980 971 745 156 BbvCI CC'TCA_GC Cuts at: 2013 2333 2013 Size: 320 6251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4762 4796 1903 Size: 34 50 34 2741 34 3678 Fragments arranged by size: 3678 2741 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5673 4463 Size: 1210 5361 BclI T'GATC_A Cuts at: 1548 2125 1548 Size: 577 5994 BfrBI ATG'CAT Cuts at: 3433 3433 Size: 6571 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3401 5182 136 Size: 107 122 71 631 605 1729 1781 1525 Fragments arranged by size: 1781 1729 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3534 6566 3534 Size: 3032 3539 BlpI GC'TnA_GC Cuts at: 2109 3497 3988 2109 Size: 1388 491 4692 Fragments arranged by size: 4692 1388 491 Bme1580I G_kGCm'C Cuts at: 1790 2316 2537 2600 3205 3245 3717 4632 5788 Size: 526 221 63 605 40 472 915 1156 Cuts at: 5788 1790 Size: 2573 Fragments arranged by size: 2573 1156 915 605 526 472 221 63 40 BmgBI CAC'GTC Cuts at: 1318 3156 1318 Size: 1838 4733 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 3121 4302 Size: 416 53 83 399 24 1147 557 1181 Cuts at: 4302 5260 442 Size: 958 1753 Fragments arranged by size: 1753 1181 1147 958 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3137 1056 Size: 2081 4490 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2707 3196 2707 Size: 489 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3589 3635 3812 5231 1239 Size: 2350 46 177 1419 2579 Fragments arranged by size: 2579 2350 1419 177 46 Bpu10I CC'TnA_GC Cuts at: 2013 2333 2013 Size: 320 6251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4696 5561 5859 4696 Size: 865 298 5408 Fragments arranged by size: 5408 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3083 5234 882 Size: 617 1584 2151 2219 Fragments arranged by size: 2219 2151 1584 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 4362 493 Size: 711 1296 1862 2702 Fragments arranged by size: 2702 1862 1296 711 BsaBI GATnn'nnATC Cuts at: 2474 6565 2474 Size: 4091 2480 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4378 4760 5395 275 Size: 53 83 186 936 2845 382 635 1451 Fragments arranged by size: 2845 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3271 3555 3630 5004 5402 5676 5823 6234 3271 Size: 284 75 1374 398 274 147 411 3608 Fragments arranged by size: 3608 1374 411 398 284 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 3354 3384 3608 Size: 30 165 30 1362 30 559 30 224 Cuts at: 3608 3638 1178 Size: 30 4111 Fragments arranged by size: 4111 1362 559 224 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 2168 2171 2375 1186 Size: 815 167 3 204 5382 Fragments arranged by size: 5382 815 204 167 3 BseYI C'CCAG_C Cuts at: 2449 2858 2897 3507 3514 5774 6270 2449 Size: 409 39 610 7 2260 496 2750 Fragments arranged by size: 2750 2260 610 496 409 39 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3208 1163 Size: 573 1472 4526 Fragments arranged by size: 4526 1472 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2347 2850 4021 4782 5810 1540 Size: 275 186 346 503 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 503 346 275 186 BsiHKAI G_wGCw'C Cuts at: 2796 3182 4632 4717 5788 2796 Size: 386 1450 85 1071 3579 Fragments arranged by size: 3579 1450 1071 386 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2456 2537 2600 2796 3182 3205 Size: 399 526 140 81 63 196 386 23 Cuts at: 3205 3245 3717 3890 4632 4717 5788 1391 Size: 40 472 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 472 399 386 196 173 140 85 81 63 40 23 BspEI T'CCGG_A Cuts at: 3271 3555 6234 3271 Size: 284 2679 3608 Fragments arranged by size: 3608 2679 284 BspHI T'CATG_A Cuts at: 2520 4460 6190 2520 Size: 1940 1730 2901 Fragments arranged by size: 2901 1940 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 3325 3926 844 Size: 1832 649 601 3489 Fragments arranged by size: 3489 1832 649 601 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2937 5066 5240 65 Size: 1183 1689 2129 174 1396 Fragments arranged by size: 2129 1689 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 5215 5402 1700 Size: 110 950 2455 187 2869 Fragments arranged by size: 2869 2455 950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6571 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 5375 5375 Size: 6571 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2764 3613 2257 Size: 507 849 5215 Fragments arranged by size: 5215 849 507 BstYI r'GATC_y Cuts at: 1972 3534 3837 3901 4268 4653 4670 6122 6208 Size: 1562 303 64 367 385 17 1452 86 Cuts at: 6208 6220 6566 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1562 1452 385 367 346 303 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2201 2823 2201 Size: 622 5949 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6019 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2574 2644 487 Size: 683 56 1348 70 4414 Fragments arranged by size: 4414 1348 683 70 56 BtsI GCAGTG_nn' Cuts at: 2088 2707 4087 4881 4901 2088 Size: 619 1380 794 20 3758 Fragments arranged by size: 3758 1380 794 619 20 ClaI AT'CG_AT Cuts at: 1554 3636 1554 Size: 2082 4489 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3875 3875 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2259 2505 Size: 54 1425 50 275 18 210 219 246 Cuts at: 2505 2754 2766 2847 2883 3084 3378 3584 4018 Size: 249 12 81 36 201 294 206 434 Cuts at: 4018 4907 6536 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 434 294 275 249 246 219 210 206 201 81 54 50 43 36 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2847 4018 1537 Size: 275 1035 1171 4090 Fragments arranged by size: 4090 1171 1035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 4501 1353 Size: 831 2317 3423 Fragments arranged by size: 3423 2317 831 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3180 3180 Size: 6571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 3006 3123 3589 3635 3812 4634 Size: 393 599 775 117 466 46 177 822 Cuts at: 4634 5231 6018 6286 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 822 787 775 599 597 466 393 268 177 117 46 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 6571 EcoRI G'AATT_C Cuts at: 3752 3880 4258 3752 Size: 128 378 6065 Fragments arranged by size: 6065 378 128 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 6571 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 2865 5718 1678 Size: 1187 2853 2531 Fragments arranged by size: 2853 2531 1187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2673 2705 5212 5244 5286 Size: 32 135 32 1101 32 2507 32 42 Cuts at: 5286 5318 1373 Size: 32 2626 Fragments arranged by size: 2626 2507 1101 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2626 3547 3915 4161 669 Size: 988 281 688 921 368 246 3079 Fragments arranged by size: 3079 988 921 688 368 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6571 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5685 5869 1305 Size: 4380 184 2007 Fragments arranged by size: 4380 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2507 2756 2768 2885 Size: 54 1425 343 429 246 249 12 117 Cuts at: 2885 3586 10 Size: 701 2995 Fragments arranged by size: 2995 1425 701 429 343 249 246 117 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2139 3722 4529 4888 5047 518 Size: 648 973 1583 807 359 159 2042 Fragments arranged by size: 2042 1583 973 807 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2449 3351 3401 3987 4664 5812 6057 Size: 411 432 902 50 586 677 1148 245 Cuts at: 6057 1606 Size: 2120 Fragments arranged by size: 2120 1148 902 677 586 432 411 245 50 NaeI GCC'GGC Cuts at: 2762 2762 Size: 6571 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6019 NdeI CA'TA_TG Cuts at: 387 387 Size: 6571 NgoMIV G'CCGG_C Cuts at: 2760 2760 Size: 6571 NheI G'CTAG_C Cuts at: 1052 3133 1052 Size: 2081 4490 NotI GC'GGCC_GC Cuts at: 2847 4018 2847 Size: 1171 5400 NsiI A_TGCA'T Cuts at: 3435 3435 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 2515 2800 3929 5474 1132 Size: 97 1286 285 1129 1545 2229 Fragments arranged by size: 2229 1545 1286 1129 285 97 PasI CC'CwG_GG Cuts at: 1348 2250 2396 1348 Size: 902 146 5523 Fragments arranged by size: 5523 902 146 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 2993 3593 1108 Size: 1482 403 600 4086 Fragments arranged by size: 4086 1482 600 403 PfoI T'CCnGG_A Cuts at: 2544 3158 2544 Size: 614 5957 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2363 2395 3608 3640 4640 4672 6153 6185 2363 Size: 32 1213 32 1000 32 1481 32 2749 Fragments arranged by size: 2749 1481 1213 1000 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspXI vC'TCGA_Gb Cuts at: 3214 3214 Size: 6571 PstI C_TGCA'G Cuts at: 830 3409 3923 830 Size: 2579 514 3478 Fragments arranged by size: 3478 2579 514 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 PvuII CAG'CTG Cuts at: 2017 3351 2017 Size: 1334 5237 SacI G_AGCT'C Cuts at: 3182 3182 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SfcI C'TryA_G Cuts at: 826 1045 3405 3919 5054 5735 5926 826 Size: 219 2360 514 1135 681 191 1471 Fragments arranged by size: 2360 1471 1135 681 514 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 2908 3898 1872 Size: 1036 990 4545 Fragments arranged by size: 4545 1036 990 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 3214 4675 5576 5838 820 Size: 197 926 153 1118 1461 901 262 1553 Fragments arranged by size: 1553 1461 1118 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 4362 493 Size: 3869 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 2515 3929 1132 Size: 1383 1414 3774 Fragments arranged by size: 3774 1414 1383 SspI AAT'ATT Cuts at: 5 52 4495 5 Size: 47 4443 2081 Fragments arranged by size: 4443 2081 47 StuI AGG'CCT Cuts at: 1517 2331 3708 1517 Size: 814 1377 4380 Fragments arranged by size: 4380 1377 814 StyI C'CwwG_G Cuts at: 513 1065 2769 3681 3747 4010 513 Size: 552 1704 912 66 263 3074 Fragments arranged by size: 3074 1704 912 552 263 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 2333 3528 4598 4615 4768 4953 Size: 1255 726 27 1195 1070 17 153 185 Cuts at: 4953 5398 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1195 1070 726 445 185 153 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4817 6529 96 Size: 274 80 33 51 1443 2840 1712 138 Fragments arranged by size: 2840 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2765 2797 3354 3386 6531 6563 2765 Size: 32 557 32 3145 32 2773 Fragments arranged by size: 3145 2773 557 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 2096 3214 1943 Size: 153 1118 5300 Fragments arranged by size: 5300 1118 153 XmaI C'CCGG_G Cuts at: 1870 2906 3896 1870 Size: 1036 990 4545 Fragments arranged by size: 4545 1036 990 XmnI GAAnn'nnTTC Cuts at: 2378 3279 4700 2378 Size: 901 1421 4249 Fragments arranged by size: 4249 1421 901 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI