################################## # NO CUT ENZYMES ( 223 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp509I TspRI Tth111I TthHB8I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 301 GTAGAC GT^[AC][GT]AC AfaI 297 GTAC GT^AC AlwI 148 GGATC GGATC(4/5) BanI 232 GGCACC G^G[CT][GA]CC BbvI 64 GCTGC GCAGC(8/12) BfaI 247 CTAG C^TAG BsaAI 298 TACGTA [CT]AC^GT[GA] BsrI 184 CCAGT ACTGG(1/-1) Cac8I 126 GCAAGC GC[ACGT]^[ACGT]GC DpnII 149 GATC ^GATC Fnu4HI 64 GCTGC GC^[ACGT]GC HinfI 177 GATTC G^A[ACGT]TC HphI 280 GGTGA GGTGA(8/7) ItaI 64 GCTGC GC^[ACGT]GC MaeI 247 CTAG C^TAG MaeII 299 ACGT A^CGT MboI 149 GATC ^GATC MwoI 4 GCAGGACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 149 GATC ^GATC NlaIV 232 GGCACC GG[ACGT]^[ACGT]CC PflMI 220 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 297 GTAC GT^AC Sau3AI 149 GATC ^GATC SmlI 110 CTCAAG C^T[CT][GA]AG SnaBI 298 TACGTA TAC^GTA TfiI 177 GATTC G^A[AT]TC TseI 64 GCTGC G^C[AT]GC Tsp45I 281 GTGAC ^GT[GC]AC Van91I 220 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 246 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BsiYI 42 CCGAGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 220 CCAGAATATGG BslI 42 CCGAGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 220 CCAGAATATGG MaeIII 76 GTAAC ^GT[ACGT]AC 281 GTGAC MboII 27 TCTTC GAAGA(8/7) 213 TCTTC MnlI 86 GAGG CCTC(7/6) 230 GAGG ######################################### # 3 OR MORE CUT ENZYMES ( 0 enzymes) # #########################################