(Circular) (Six-base) MAPSORT of: pFN21AE2235.seq Check: 8946 from: 1 to: 5011 pFN21AE2235 5011 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2821 278 Size: 53 83 186 936 1285 2468 Fragments arranged by size: 2468 1285 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 2354 1937 Size: 370 47 4594 Fragments arranged by size: 4594 370 47 Acc65I G'GTAC_C Cuts at: 2332 2332 Size: 5011 AclI AA'CG_TT Cuts at: 1563 3138 3511 1563 Size: 1575 373 3063 Fragments arranged by size: 3063 1575 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3074 4458 4726 1632 Size: 1442 1384 268 1917 Fragments arranged by size: 1917 1442 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4814 AflIII A'CryG_T Cuts at: 1203 3910 4887 1203 Size: 2707 977 1327 Fragments arranged by size: 2707 1327 977 AgeI A'CCGG_T Cuts at: 3842 3842 Size: 5011 AhdI GACnn_n'nnGTC Cuts at: 3740 3740 Size: 5011 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5011 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5011 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2199 2231 2199 Size: 32 4979 AlwNI CAG_nnn'CTG Cuts at: 2754 4326 2754 Size: 1572 3439 ApaLI G'TGCA_C Cuts at: 3068 4224 3068 Size: 1156 3855 ApoI r'AATT_y Cuts at: 1493 2320 2546 2698 4807 1493 Size: 827 226 152 2109 1697 Fragments arranged by size: 2109 1697 827 226 152 AseI AT'TA_AT Cuts at: 160 1032 3565 160 Size: 872 2533 1606 Fragments arranged by size: 2533 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5011 AvaI C'yCGr_G Cuts at: 1870 1943 2336 1870 Size: 73 393 4545 Fragments arranged by size: 4545 393 73 BamHI G'GATC_C Cuts at: 2341 2708 2341 Size: 367 4644 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 2332 3787 618 Size: 325 217 627 450 95 1455 1842 Fragments arranged by size: 1842 1455 627 450 325 217 95 BanII G_rGCy'C Cuts at: 1391 2330 1391 Size: 939 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3202 3236 1903 Size: 34 1265 34 3678 Fragments arranged by size: 3678 1265 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2903 4113 2903 Size: 1210 3801 BclI T'GATC_A Cuts at: 1548 1548 Size: 5011 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3622 136 Size: 107 122 71 631 605 1950 1525 Fragments arranged by size: 1950 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5006 5006 Size: 5011 BlpI GC'TnA_GC Cuts at: 2428 2428 Size: 5011 Bme1580I G_kGCm'C Cuts at: 1790 3072 4228 1790 Size: 1282 1156 2573 Fragments arranged by size: 2573 1282 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5011 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2742 3700 442 Size: 416 53 83 399 24 1325 958 1753 Fragments arranged by size: 1753 1325 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5011 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3671 1239 Size: 2432 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2100 3136 4001 4299 2100 Size: 1036 865 298 2812 Fragments arranged by size: 2812 1036 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3674 882 Size: 617 2175 2219 Fragments arranged by size: 2219 2175 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 2802 493 Size: 711 1101 497 2702 Fragments arranged by size: 2702 1101 711 497 BsaBI GATnn'nnATC Cuts at: 5005 5005 Size: 5011 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2818 3200 3835 275 Size: 53 83 186 936 1285 382 635 1451 Fragments arranged by size: 1451 1285 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3444 3842 4116 4263 4674 3444 Size: 398 274 147 411 3781 Fragments arranged by size: 3781 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2199 2229 1178 Size: 30 165 30 796 30 3960 Fragments arranged by size: 3960 796 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5011 BseYI C'CCAG_C Cuts at: 4214 4710 4214 Size: 496 4515 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2461 3222 4250 1540 Size: 275 186 460 761 1028 2301 Fragments arranged by size: 2301 1028 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 3072 3157 4228 3072 Size: 85 1071 3855 Fragments arranged by size: 3855 1071 85 BsmI GAATG_Cn' Cuts at: 2522 2615 2522 Size: 93 4918 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 3072 3157 4228 1391 Size: 399 540 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 85 BspEI T'CCGG_A Cuts at: 4674 4674 Size: 5011 BspHI T'CATG_A Cuts at: 2900 4630 2900 Size: 1730 3281 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2366 844 Size: 1522 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2898 1394 Size: 429 1075 3507 Fragments arranged by size: 3507 1075 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3506 3680 65 Size: 1183 2258 174 1396 Fragments arranged by size: 2258 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3655 3842 1700 Size: 110 1845 187 2869 Fragments arranged by size: 2869 1845 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5011 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5011 BssSI C'ACGA_G Cuts at: 3071 4083 3071 Size: 1012 3999 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5011 BstBI TT'CG_AA Cuts at: 3815 3815 Size: 5011 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5011 BstYI r'GATC_y Cuts at: 1972 2341 2708 3093 3110 4562 4648 4660 5006 Size: 369 367 385 17 1452 86 12 346 Cuts at: 5006 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 369 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3520 Fragments arranged by size: 3520 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4272 Fragments arranged by size: 4272 683 56 BtsI GCAGTG_nn' Cuts at: 2527 3321 3341 2527 Size: 794 20 4197 Fragments arranged by size: 4197 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5011 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4976 DraI TTT'AAA Cuts at: 2315 2315 Size: 5011 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4018 809 Size: 507 2702 1802 Fragments arranged by size: 2702 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2458 3347 4976 Size: 54 1425 50 275 18 628 889 1629 Cuts at: 4976 8 Size: 43 Fragments arranged by size: 1629 1425 889 628 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2458 1537 Size: 275 646 4090 Fragments arranged by size: 4090 646 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2941 1353 Size: 1588 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3598 3972 4118 387 Size: 1064 138 2009 374 146 1280 Fragments arranged by size: 2009 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3074 3671 4458 4726 1239 Size: 393 1442 597 787 268 1524 Fragments arranged by size: 1524 1442 787 597 393 268 EcoRI G'AATT_C Cuts at: 2320 2698 2320 Size: 378 4633 FspI TGC'GCA Cuts at: 3517 3517 Size: 5011 HaeII r_GCGC'y Cuts at: 1678 4158 1678 Size: 2480 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3652 3684 3726 3758 1373 Size: 32 135 32 2080 32 42 32 2626 Fragments arranged by size: 2626 2080 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2355 2601 669 Size: 988 281 417 246 3079 Fragments arranged by size: 3079 988 417 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5011 HpaI GTT'AAC Cuts at: 2601 2601 Size: 5011 KpnI G_GTAC'C Cuts at: 2336 2336 Size: 5011 MfeI C'AATT_G Cuts at: 2610 2610 Size: 5011 MluI A'CGCG_T Cuts at: 4887 4887 Size: 5011 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2131 2185 4125 4309 1305 Size: 826 54 1940 184 2007 Fragments arranged by size: 2007 1940 826 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3189 Fragments arranged by size: 3189 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2969 3328 3487 518 Size: 648 1803 359 159 2042 Fragments arranged by size: 2042 1803 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2427 3104 4252 4497 1606 Size: 821 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3520 Fragments arranged by size: 3520 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5011 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5011 NotI GC'GGCC_GC Cuts at: 2458 2458 Size: 5011 NspI r_CATG'y Cuts at: 1132 1229 2369 3914 1132 Size: 97 1140 1545 2229 Fragments arranged by size: 2229 1545 1140 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5011 PciI A'CATG_T Cuts at: 3910 3910 Size: 5011 PflMI CCAn_nnn'nTGG Cuts at: 1108 2231 1108 Size: 1123 3888 PmeI GTTT'AAAC Cuts at: 2315 2315 Size: 5011 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2199 2231 3080 3112 4593 4625 2199 Size: 32 849 32 1481 32 2585 Fragments arranged by size: 2585 1481 849 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5011 PsiI TTA'TAA Cuts at: 2581 2581 Size: 5011 PstI C_TGCA'G Cuts at: 830 2363 830 Size: 1533 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5011 SalI G'TCGA_C Cuts at: 1936 2353 1936 Size: 417 4594 SbfI CC_TGCA'GG Cuts at: 2363 2363 Size: 5011 ScaI AGT'ACT Cuts at: 3259 4971 3259 Size: 1712 3299 SfcI C'TryA_G Cuts at: 826 1045 2359 3494 4175 4366 826 Size: 219 1314 1135 681 191 1471 Fragments arranged by size: 1471 1314 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5011 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5011 SmaI CCC'GGG Cuts at: 1872 2338 1872 Size: 466 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2115 3115 4016 4278 820 Size: 197 926 172 1000 901 262 1553 Fragments arranged by size: 1553 1000 926 901 262 197 172 SnaBI TAC'GTA Cuts at: 493 2305 2802 493 Size: 1812 497 2702 Fragments arranged by size: 2702 1812 497 SpeI A'CTAG_T Cuts at: 152 152 Size: 5011 SphI G_CATG'C Cuts at: 1132 2369 1132 Size: 1237 3774 SspI AAT'ATT Cuts at: 5 52 2935 5 Size: 47 2883 2081 Fragments arranged by size: 2883 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5011 StyI C'CwwG_G Cuts at: 513 1065 2004 2450 513 Size: 552 939 446 3074 Fragments arranged by size: 3074 939 552 446 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 3038 3055 3208 3393 3838 325 Size: 1255 481 977 17 153 185 445 1498 Fragments arranged by size: 1498 1255 977 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3257 4969 96 Size: 274 80 33 51 1443 1280 1712 138 Fragments arranged by size: 1712 1443 1280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4971 5003 4971 Size: 32 4979 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5011 XbaI T'CTAG_A Cuts at: 2251 2347 2251 Size: 96 4915 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5011 XmaI C'CCGG_G Cuts at: 1870 2336 1870 Size: 466 4545 XmnI GAAnn'nnTTC Cuts at: 3140 3140 Size: 5011 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2819 276 Size: 53 83 186 936 1285 2468 Fragments arranged by size: 2468 1285 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI