Restriction map for pFN21AE2235
 (Circular) (Six-base) MAPSORT of: pFN21AE2235.seq  Check: 8946  from: 1  to: 5011



pFN21AE2235  5011 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:55  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2821     278 
   Size:         53      83     186     936    1285    2468

  Fragments arranged by size: 

                2468    1285     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2307    2354    1937 
   Size:        370      47    4594

  Fragments arranged by size: 

                4594     370      47
 
Acc65I G'GTAC_C

Cuts at:   2332    2332 
   Size:       5011
 
AclI AA'CG_TT

Cuts at:   1563    3138    3511    1563 
   Size:       1575     373    3063

  Fragments arranged by size: 

                3063    1575     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3074    4458    4726    1632 
   Size:       1442    1384     268    1917

  Fragments arranged by size: 

                1917    1442    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4814
 
AflIII A'CryG_T

Cuts at:   1203    3910    4887    1203 
   Size:       2707     977    1327

  Fragments arranged by size: 

                2707    1327     977
 
AgeI A'CCGG_T

Cuts at:   3842    3842 
   Size:       5011
 
AhdI GACnn_n'nnGTC

Cuts at:   3740    3740 
   Size:       5011
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5011
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5011
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2199    2231    2199 
   Size:         32    4979
 
AlwNI CAG_nnn'CTG

Cuts at:   2754    4326    2754 
   Size:       1572    3439
 
ApaLI G'TGCA_C

Cuts at:   3068    4224    3068 
   Size:       1156    3855
 
ApoI r'AATT_y

Cuts at:   1493    2320    2546    2698    4807    1493 
   Size:        827     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     827     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3565     160 
   Size:        872    2533    1606

  Fragments arranged by size: 

                2533    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5011
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2336    1870 
   Size:         73     393    4545

  Fragments arranged by size: 

                4545     393      73
 
BamHI G'GATC_C

Cuts at:   2341    2708    2341 
   Size:        367    4644
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2237    2332    3787     618 
   Size:        325     217     627     450      95    1455    1842

  Fragments arranged by size: 

                1842    1455     627     450     325     217      95
 
BanII G_rGCy'C

Cuts at:   1391    2330    1391 
   Size:        939    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4040
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3202    3236    1903 
   Size:         34    1265      34    3678

  Fragments arranged by size: 

                3678    1265      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2903    4113    2903 
   Size:       1210    3801
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5011
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3622     136 
   Size:        107     122      71     631     605    1950    1525

  Fragments arranged by size: 

                1950    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5006    5006 
   Size:       5011
 
BlpI GC'TnA_GC

Cuts at:   2428    2428 
   Size:       5011
 
Bme1580I G_kGCm'C

Cuts at:   1790    3072    4228    1790 
   Size:       1282    1156    2573

  Fragments arranged by size: 

                2573    1282    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5011
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2742    3700     442 
   Size:        416      53      83     399      24    1325     958    1753

  Fragments arranged by size: 

                1753    1325     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5011
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3671    1239 
   Size:       2432    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2100    3136    4001    4299    2100 
   Size:       1036     865     298    2812

  Fragments arranged by size: 

                2812    1036     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3674     882 
   Size:        617    2175    2219

  Fragments arranged by size: 

                2219    2175     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2305    2802     493 
   Size:        711    1101     497    2702

  Fragments arranged by size: 

                2702    1101     711     497
 
BsaBI GATnn'nnATC

Cuts at:   5005    5005 
   Size:       5011
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2818    3200    3835     275 
   Size:         53      83     186     936    1285     382     635    1451

  Fragments arranged by size: 

                1451    1285     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3444    3842    4116    4263    4674    3444 
   Size:        398     274     147     411    3781

  Fragments arranged by size: 

                3781     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2199    2229    1178 
   Size:         30     165      30     796      30    3960

  Fragments arranged by size: 

                3960     796     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5011
 
BseYI C'CCAG_C

Cuts at:   4214    4710    4214 
   Size:        496    4515
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4438
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2461    3222    4250    1540 
   Size:        275     186     460     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     460     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3072    3157    4228    3072 
   Size:         85    1071    3855

  Fragments arranged by size: 

                3855    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2522    2615    2522 
   Size:         93    4918
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2330    3072    3157    4228    1391 
   Size:        399     540     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     540     399      85
 
BspEI T'CCGG_A

Cuts at:   4674    4674 
   Size:       5011
 
BspHI T'CATG_A

Cuts at:   2900    4630    2900 
   Size:       1730    3281
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2366     844 
   Size:       1522    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2898    1394 
   Size:        429    1075    3507

  Fragments arranged by size: 

                3507    1075     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3506    3680      65 
   Size:       1183    2258     174    1396

  Fragments arranged by size: 

                2258    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3655    3842    1700 
   Size:        110    1845     187    2869

  Fragments arranged by size: 

                2869    1845     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5011
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5011
 
BssSI C'ACGA_G

Cuts at:   3071    4083    3071 
   Size:       1012    3999
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5011
 
BstBI TT'CG_AA

Cuts at:   3815    3815 
   Size:       5011
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5011
 
BstYI r'GATC_y

Cuts at:   1972    2341    2708    3093    3110    4562    4648    4660    5006 
   Size:        369     367     385      17    1452      86      12     346

Cuts at:   5006    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     369     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3520

  Fragments arranged by size: 

                3520     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4272

  Fragments arranged by size: 

                4272     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2527    3321    3341    2527 
   Size:        794      20    4197

  Fragments arranged by size: 

                4197     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5011
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4976
 
DraI TTT'AAA

Cuts at:   2315    2315 
   Size:       5011
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4018     809 
   Size:        507    2702    1802

  Fragments arranged by size: 

                2702    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2458    3347    4976 
   Size:         54    1425      50     275      18     628     889    1629

Cuts at:   4976       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     628     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2458    1537 
   Size:        275     646    4090

  Fragments arranged by size: 

                4090     646     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2941    1353 
   Size:       1588    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3598    3972    4118     387 
   Size:       1064     138    2009     374     146    1280

  Fragments arranged by size: 

                2009    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3074    3671    4458    4726    1239 
   Size:        393    1442     597     787     268    1524

  Fragments arranged by size: 

                1524    1442     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2320    2698    2320 
   Size:        378    4633
 
FspI TGC'GCA

Cuts at:   3517    3517 
   Size:       5011
 
HaeII r_GCGC'y

Cuts at:   1678    4158    1678 
   Size:       2480    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3652    3684    3726    3758    1373 
   Size:         32     135      32    2080      32      42      32    2626

  Fragments arranged by size: 

                2626    2080     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2355    2601     669 
   Size:        988     281     417     246    3079

  Fragments arranged by size: 

                3079     988     417     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5011
 
HpaI GTT'AAC

Cuts at:   2601    2601 
   Size:       5011
 
KpnI G_GTAC'C

Cuts at:   2336    2336 
   Size:       5011
 
MfeI C'AATT_G

Cuts at:   2610    2610 
   Size:       5011
 
MluI A'CGCG_T

Cuts at:   4887    4887 
   Size:       5011
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2131    2185    4125    4309    1305 
   Size:        826      54    1940     184    2007

  Fragments arranged by size: 

                2007    1940     826     184      54
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3189

  Fragments arranged by size: 

                3189    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2969    3328    3487     518 
   Size:        648    1803     359     159    2042

  Fragments arranged by size: 

                2042    1803     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2427    3104    4252    4497    1606 
   Size:        821     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     821     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3520

  Fragments arranged by size: 

                3520     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5011
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5011
 
NotI GC'GGCC_GC

Cuts at:   2458    2458 
   Size:       5011
 
NspI r_CATG'y

Cuts at:   1132    1229    2369    3914    1132 
   Size:         97    1140    1545    2229

  Fragments arranged by size: 

                2229    1545    1140      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5011
 
PciI A'CATG_T

Cuts at:   3910    3910 
   Size:       5011
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2231    1108 
   Size:       1123    3888
 
PmeI GTTT'AAAC

Cuts at:   2315    2315 
   Size:       5011
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2199    2231    3080    3112    4593    4625    2199 
   Size:         32     849      32    1481      32    2585

  Fragments arranged by size: 

                2585    1481     849      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5011
 
PsiI TTA'TAA

Cuts at:   2581    2581 
   Size:       5011
 
PstI C_TGCA'G

Cuts at:    830    2363     830 
   Size:       1533    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5011
 
SalI G'TCGA_C

Cuts at:   1936    2353    1936 
   Size:        417    4594
 
SbfI CC_TGCA'GG

Cuts at:   2363    2363 
   Size:       5011
 
ScaI AGT'ACT

Cuts at:   3259    4971    3259 
   Size:       1712    3299
 
SfcI C'TryA_G

Cuts at:    826    1045    2359    3494    4175    4366     826 
   Size:        219    1314    1135     681     191    1471

  Fragments arranged by size: 

                1471    1314    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5011
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5011
 
SmaI CCC'GGG

Cuts at:   1872    2338    1872 
   Size:        466    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2115    3115    4016    4278     820 
   Size:        197     926     172    1000     901     262    1553

  Fragments arranged by size: 

                1553    1000     926     901     262     197     172
 
SnaBI TAC'GTA

Cuts at:    493    2305    2802     493 
   Size:       1812     497    2702

  Fragments arranged by size: 

                2702    1812     497
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5011
 
SphI G_CATG'C

Cuts at:   1132    2369    1132 
   Size:       1237    3774
 
SspI AAT'ATT

Cuts at:      5      52    2935       5 
   Size:         47    2883    2081

  Fragments arranged by size: 

                2883    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5011
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2450     513 
   Size:        552     939     446    3074

  Fragments arranged by size: 

                3074     939     552     446
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2061    3038    3055    3208    3393    3838     325 
   Size:       1255     481     977      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     977     481     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3257    4969      96 
   Size:        274      80      33      51    1443    1280    1712     138

  Fragments arranged by size: 

                1712    1443    1280     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4971    5003    4971 
   Size:         32    4979
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5011
 
XbaI T'CTAG_A

Cuts at:   2251    2347    2251 
   Size:         96    4915
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5011
 
XmaI C'CCGG_G

Cuts at:   1870    2336    1870 
   Size:        466    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3140    3140 
   Size:       5011
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2819     276 
   Size:         53      83     186     936    1285    2468

  Fragments arranged by size: 

                2468    1285     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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