(Linear) (Six-base) MAPSORT of: KIEE1317.seq Check: 7118 from: 1 to: 2214 KIEE1317.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AatII G_ACGT'C Cuts at: 0 1415 2214 Size: 1415 799 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1427 2214 Size: 311 1116 787 Fragments arranged by size: 1116 787 311 AflIII A'CryG_T Cuts at: 0 905 2214 Size: 905 1309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1455 2214 Size: 1455 759 AlwNI CAG_nnn'CTG Cuts at: 0 219 2214 Size: 219 1995 ApaI G_GGCC'C Cuts at: 0 18 2214 Size: 18 2196 ApaLI G'TGCA_C Cuts at: 0 765 2214 Size: 765 1449 ApoI r'AATT_y Cuts at: 0 543 558 574 2214 Size: 543 15 16 1640 Fragments arranged by size: 1640 543 16 15 AvaI C'yCGr_G Cuts at: 0 548 2214 Size: 548 1666 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1079 1112 2214 Size: 1079 33 1102 Fragments arranged by size: 1102 1079 33 BamHI G'GATC_C Cuts at: 0 638 2214 Size: 638 1576 BanII G_rGCy'C Cuts at: 0 18 750 2214 Size: 18 732 1464 Fragments arranged by size: 1464 732 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 1415 2214 Size: 1415 799 BbvCI CC'TCA_GC Cuts at: 0 206 2088 2214 Size: 206 1882 126 Fragments arranged by size: 1882 206 126 BclI T'GATC_A Cuts at: 0 734 1832 1847 2214 Size: 734 1098 15 367 Fragments arranged by size: 1098 734 367 15 BglI GCCn_nnn'nGGC Cuts at: 0 22 1660 2214 Size: 22 1638 554 Fragments arranged by size: 1638 554 22 BglII A'GATC_T Cuts at: 0 2106 2214 Size: 2106 108 BlpI GC'TnA_GC Cuts at: 0 749 2214 Size: 749 1465 Bme1580I G_kGCm'C Cuts at: 0 18 392 769 2214 Size: 18 374 377 1445 Fragments arranged by size: 1445 377 374 18 BmgBI CAC'GTC Cuts at: 0 198 237 2061 2214 Size: 198 39 1824 153 Fragments arranged by size: 1824 198 153 39 BmrI ACTGGGnnnn_n' Cuts at: 0 386 1245 2214 Size: 386 859 969 Fragments arranged by size: 969 859 386 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 769 2214 Size: 769 1445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1043 1565 1728 2214 Size: 1043 522 163 486 Fragments arranged by size: 1043 522 486 163 Bpu10I CC'TnA_GC Cuts at: 0 206 2088 2214 Size: 206 1882 126 Fragments arranged by size: 1882 206 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 1091 2214 Size: 94 997 1123 Fragments arranged by size: 1123 997 94 BsaI GGTCTCn'nnnn_ Cuts at: 0 545 2214 Size: 545 1669 BsaAI yAC'GTr Cuts at: 0 908 2214 Size: 908 1306 BsaBI GATnn'nnATC Cuts at: 0 1516 2214 Size: 1516 698 BsaHI Gr'CG_yC Cuts at: 0 1412 2214 Size: 1412 802 BsaWI w'CCGG_w Cuts at: 0 296 727 2147 2214 Size: 296 431 1420 67 Fragments arranged by size: 1420 431 296 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 238 241 268 271 1690 1720 1726 1756 Size: 238 3 27 3 1419 30 6 30 Cuts at: 1756 2214 Size: 458 Fragments arranged by size: 1419 458 238 30 30 27 6 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 246 2214 Size: 246 1968 BseYI C'CCAG_C Cuts at: 0 1226 2214 Size: 1226 988 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1140 1698 2083 2214 Size: 1140 558 385 131 Fragments arranged by size: 1140 558 385 131 BsiHKAI G_wGCw'C Cuts at: 0 271 750 769 893 1056 2214 Size: 271 479 19 124 163 1158 Fragments arranged by size: 1158 479 271 163 124 19 Bsp1286I G_dGCh'C Cuts at: 0 18 271 392 750 769 893 1056 2214 Size: 18 253 121 358 19 124 163 1158 Fragments arranged by size: 1158 358 253 163 124 121 19 18 BspEI T'CCGG_A Cuts at: 0 296 2147 2214 Size: 296 1851 67 Fragments arranged by size: 1851 296 67 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1153 2214 Size: 1153 1061 BsrDI GCAATG_nn' Cuts at: 0 1570 1835 1850 2024 2214 Size: 1570 265 15 174 190 Fragments arranged by size: 1570 265 190 174 15 BsrGI T'GTAC_A Cuts at: 0 2135 2214 Size: 2135 79 BstAPI GCAn_nnn'nTGC Cuts at: 0 216 2214 Size: 216 1998 BstEII G'GTnAC_C Cuts at: 0 678 2214 Size: 678 1536 BstYI r'GATC_y Cuts at: 0 299 638 1582 1959 2106 2214 Size: 299 339 944 377 147 108 Fragments arranged by size: 944 377 339 299 147 108 BtgI C'CryG_G Cuts at: 0 881 1792 2168 2214 Size: 881 911 376 46 Fragments arranged by size: 911 881 376 46 BtsI GCAGTG_nn' Cuts at: 0 172 356 1157 1820 2214 Size: 172 184 801 663 394 Fragments arranged by size: 801 663 394 184 172 ClaI AT'CG_AT Cuts at: 0 1297 2214 Size: 1297 917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1514 1549 2214 Size: 1514 35 665 Fragments arranged by size: 1514 665 35 DraI TTT'AAA Cuts at: 0 1334 2214 Size: 1334 880 DraIII CAC_nnn'GTG Cuts at: 0 32 2214 Size: 32 2182 DrdI GACnn_nn'nnGTC Cuts at: 0 1177 2214 Size: 1177 1037 EaeI y'GGCC_r Cuts at: 0 878 1856 2214 Size: 878 978 358 Fragments arranged by size: 978 878 358 EarI CTCTTCn'nnn_ Cuts at: 0 112 259 2214 Size: 112 147 1955 Fragments arranged by size: 1955 147 112 EcoICRI GAG'CTC Cuts at: 0 748 2214 Size: 748 1466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1043 1427 1565 1728 2214 Size: 311 732 384 138 163 486 Fragments arranged by size: 732 486 384 311 163 138 EcoNI CCTnn'n_nnAGG Cuts at: 0 790 2214 Size: 790 1424 EcoO109I rG'GnC_Cy Cuts at: 0 474 2214 Size: 474 1740 EcoRI G'AATT_C Cuts at: 0 543 574 2214 Size: 543 31 1640 Fragments arranged by size: 1640 543 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1262 1294 1824 1856 1939 1971 2214 Size: 1262 32 530 32 83 32 243 Fragments arranged by size: 1262 530 243 83 32 32 32 HincII GTy'rAC Cuts at: 0 111 450 957 2214 Size: 111 339 507 1257 Fragments arranged by size: 1257 507 339 111 MfeI C'AATT_G Cuts at: 0 491 2214 Size: 491 1723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1115 1964 1986 2214 Size: 1115 849 22 228 Fragments arranged by size: 1115 849 228 22 MscI TGG'CCA Cuts at: 0 880 2214 Size: 880 1334 MslI CAynn'nnrTG Cuts at: 0 321 730 775 880 1828 2214 Size: 321 409 45 105 948 386 Fragments arranged by size: 948 409 386 321 105 45 MspA1I CmG'CkG Cuts at: 0 2092 2214 Size: 2092 122 NcoI C'CATG_G Cuts at: 0 881 1792 2214 Size: 881 911 422 Fragments arranged by size: 911 881 422 NdeI CA'TA_TG Cuts at: 0 644 2214 Size: 644 1570 PasI CC'CwG_GG Cuts at: 0 398 2214 Size: 398 1816 PflMI CCAn_nnn'nTGG Cuts at: 0 32 375 2214 Size: 32 343 1839 Fragments arranged by size: 1839 343 32 PmlI CAC'GTG Cuts at: 0 908 2214 Size: 908 1306 PspOMI G'GGCC_C Cuts at: 0 14 2214 Size: 14 2200 PstI C_TGCA'G Cuts at: 0 167 363 1164 2214 Size: 167 196 801 1050 Fragments arranged by size: 1050 801 196 167 PvuII CAG'CTG Cuts at: 0 2092 2214 Size: 2092 122 SacI G_AGCT'C Cuts at: 0 750 2214 Size: 750 1464 SapI GCTCTTCn'nnn_ Cuts at: 0 112 259 2214 Size: 112 147 1955 Fragments arranged by size: 1955 147 112 SexAI A'CCwGG_T Cuts at: 0 368 2214 Size: 368 1846 SfcI C'TryA_G Cuts at: 0 163 359 1160 2214 Size: 163 196 801 1054 Fragments arranged by size: 1054 801 196 163 SfiI GGCCn_nnn'nGGCC Cuts at: 0 22 2214 Size: 22 2192 SmlI C'TyrA_G Cuts at: 0 73 548 1106 2214 Size: 73 475 558 1108 Fragments arranged by size: 1108 558 475 73 StyI C'CwwG_G Cuts at: 0 18 429 881 935 1014 1792 1883 2214 Size: 18 411 452 54 79 778 91 331 Fragments arranged by size: 778 452 411 331 91 79 54 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 720 1672 2214 Size: 720 952 542 Fragments arranged by size: 952 720 542 TatI w'GTAC_w Cuts at: 0 325 866 2066 2135 2214 Size: 325 541 1200 69 79 Fragments arranged by size: 1200 541 325 79 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 695 727 2214 Size: 695 32 1487 Fragments arranged by size: 1487 695 32 Tth111I GACn'n_nGTC Cuts at: 0 2002 2214 Size: 2002 212 XbaI T'CTAG_A Cuts at: 0 2185 2214 Size: 2185 29 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 405 672 847 984 2214 Size: 405 267 175 137 1230 Fragments arranged by size: 1230 405 267 175 137 XhoI C'TCGA_G Cuts at: 0 548 2214 Size: 548 1666 ZraI GAC'GTC Cuts at: 0 1413 2214 Size: 1413 801 Enzymes that do cut: AatII AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstEII BstYI BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NdeI PasI PflMI PmlI PspOMI PstI PvuII SacI SapI SexAI SfcI SfiI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BanI BbeI BcgI BciVI BfrBI BmtI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI EagI EciI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XmaI XmnI