################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeIII HgaI Hin1I HincII HindII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 721 GGTACC G^GTACC AfaI 722 GTAC GT^AC AlwNI 376 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 544 ATTAAT AT^TAAT AsnI 544 ATTAAT AT^TAAT Asp718I 721 GGTACC G^GTACC AspHI 812 GAGCAC G[AT]GC[AT]^C AvaI 477 CTCGGG C^[CT]CG[GA]G BanII 480 GGGCTC G[GA]GC[CT]^C BbsI 77 GTCTTC GAAGAC(2/6) BcnI 260 CCCGG CC^[GC]GG BfaI 124 CTAG C^TAG BglII 251 AGATCT A^GATCT BpmI 241 CTGGAG CTGGAG(16/14) BpuAI 77 GTCTTC GAAGAC(2/6) BseRI 743 GAGGAG GAGGAG(10/8) BsiHKAI 812 GAGCAC G[AT]GC[AT]^C BsmFI 246 GGGAC GGGAC(10/14) BsoBI 477 CTCGGG C^[CT]CG[GA]G BstXI 640 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 695 GCGC GCG^C DraII 128 GGGTCCT [GA]G^G[ACGT]CC[CT] EarI 402 GAAGAG CTCTTC(1/4) EcoO109I 128 GGGTCCT [GA]G^G[ACGT]CC[CT] HaeII 694 GGCGCT [GA]GCGC^[CT] HapII 261 CCGG C^CGG HhaI 695 GCGC GCG^C HinP1I 695 GCGC G^CGC HindIII 278 AAGCTT A^AGCTT HpaII 261 CCGG C^CGG KpnI 721 GGTACC GGTAC^C Ksp632I 402 GAAGAG CTCTTC(1/4) MaeI 124 CTAG C^TAG MspA1I 554 CAGCTG C[AC]G^C[GT]G MspI 261 CCGG C^CGG NciI 260 CCCGG CC^[GC]GG NspI 170 GCATGC [GA]CATG^[CT] PpuMI 128 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 544 ATTAAT AT^TAAT PvuII 554 CAGCTG CAG^CTG RsaI 722 GTAC GT^AC SmlI 304 CTCAAG C^T[CT][GA]AG SphI 170 GCATGC GCATG^C Tsp45I 151 GTGAC ^GT[GC]AC TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 433 CCGC CCGC(-3/-1) 757 GCGG AlwI 21 GATCC GGATC(4/5) 443 GGATC AvaII 36 GGACC G^G[AT]CC 129 GGTCC BanI 104 GGTGCC G^G[CT][GA]CC 721 GGTACC BmyI 480 GGGCTC G[AGT]GC[ACT]^C 812 GAGCAC BsiYI 132 CCTTTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 323 CCCTTGCTCGG BslI 132 CCTTTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 323 CCCTTGCTCGG BsmAI 618 GTCTC GTCTC(1/5) 700 GTCTC Bsp1286I 480 GGGCTC G[AGT]GC[ACT]^C 812 GAGCAC Cfr13I 36 GGACC G^G[ACGT]CC 129 GGTCC DdeI 255 CTCAG C^T[ACGT]AG 808 CTAAG DsaI 97 CCATGG C^C[GA][CT]GG 640 CCATGG EcoT14I 97 CCATGG C^C[AT][AT]GG 640 CCATGG HphI 225 TCACC GGTGA(8/7) 369 TCACC MaeIII 151 GTGAC ^GT[ACGT]AC 464 GTTAC MseI 488 TTAA T^TAA 545 TTAA MslI 98 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 156 CATTATGGTG NcoI 97 CCATGG C^CATGG 640 CCATGG Sau96I 36 GGACC G^G[ACGT]CC 129 GGTCC SfaNI 526 GATGC GCATC(5/9) 669 GATGC StyI 97 CCATGG C^C[AT][AT]GG 640 CCATGG TfiI 378 GAATC G^A[AT]TC 384 GAATC Tru9I 488 TTAA T^TAA 545 TTAA XcmI 155 CCATTATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 350 CCAAAGAGCAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI BbvI BsaJI BsrI BssKI BstF5I BstNI BstYI Cac8I DpnII EcoRII Fnu4HI FokI HinfI ItaI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI TseI Tsp509I XhoII