(Circular) (Six-base) MAPSORT of: pFN21AE1554.seq Check: 5238 from: 1 to: 5524 pFN21AE1554 5524 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2726 2845 2726 Size: 119 5405 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 2426 2706 2770 3587 4971 5239 1632 Size: 437 357 280 64 817 1384 268 1917 Fragments arranged by size: 1917 1384 817 437 357 280 268 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 4423 5400 1203 Size: 3220 977 1327 Fragments arranged by size: 3220 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 2386 3267 4839 2386 Size: 881 1572 3071 Fragments arranged by size: 3071 1572 881 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 2550 4078 160 Size: 872 1518 1528 1606 Fragments arranged by size: 1606 1528 1518 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2482 2849 1870 Size: 73 539 367 4545 Fragments arranged by size: 4545 539 367 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2726 2845 4300 618 Size: 325 217 627 322 617 119 1455 1842 Fragments arranged by size: 1842 1455 627 617 325 322 217 119 BanII G_rGCy'C Cuts at: 1391 2489 2843 1391 Size: 1098 354 4072 Fragments arranged by size: 4072 1098 354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 928 Size: 971 176 4377 Fragments arranged by size: 4377 971 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4135 136 Size: 107 122 71 631 605 2463 1525 Fragments arranged by size: 2463 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2256 5519 2256 Size: 3263 2261 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 3585 4741 1790 Size: 1795 1156 2573 Fragments arranged by size: 2573 1795 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2506 3255 4213 Size: 416 53 83 399 24 1089 749 958 Cuts at: 4213 442 Size: 1753 Fragments arranged by size: 1753 1089 958 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 4184 1239 Size: 1028 1917 2579 Fragments arranged by size: 2579 1917 1028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 3649 4514 4812 2294 Size: 1355 865 298 3006 Fragments arranged by size: 3006 1355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4187 882 Size: 617 2688 2219 Fragments arranged by size: 2688 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3331 3713 4348 275 Size: 53 83 186 936 1798 382 635 1451 Fragments arranged by size: 1798 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2763 1186 Size: 1577 3947 BseYI C'CCAG_C Cuts at: 2170 2636 4727 5223 2170 Size: 466 2091 496 2471 Fragments arranged by size: 2471 2091 496 466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4951 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2974 3735 4763 1540 Size: 275 186 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2821 3585 3670 4741 2821 Size: 764 85 1071 3604 Fragments arranged by size: 3604 1071 764 85 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2489 2821 2843 3585 3670 4741 1391 Size: 399 699 332 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 699 399 332 85 22 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3411 1394 Size: 429 1588 3507 Fragments arranged by size: 3507 1588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4168 4355 1700 Size: 110 2358 187 2869 Fragments arranged by size: 2869 2358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstXI CCAn_nnnn'nTGG Cuts at: 2652 2652 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2025 2256 2448 2854 3221 3606 3623 5075 Size: 53 231 192 406 367 385 17 1452 Cuts at: 5075 5161 5173 5519 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 406 385 367 346 231 192 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2102 2645 513 Size: 552 1037 543 3392 Fragments arranged by size: 3392 1037 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2971 3860 5489 Size: 54 1425 50 275 18 1141 889 1629 Cuts at: 5489 8 Size: 43 Fragments arranged by size: 1629 1425 1141 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 3454 1353 Size: 1049 1052 3423 Fragments arranged by size: 3423 1052 1049 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2267 2426 2706 2770 3587 4184 Size: 393 437 198 159 280 64 817 597 Cuts at: 4184 4971 5239 1239 Size: 787 268 1524 Fragments arranged by size: 1524 817 787 597 437 393 280 268 198 159 64 EcoO109I rG'GnC_Cy Cuts at: 2134 2134 Size: 5524 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2703 4671 1678 Size: 1025 1968 2531 Fragments arranged by size: 2531 1968 1025 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2688 2720 4165 Size: 32 135 32 470 32 614 32 1445 Cuts at: 4165 4197 4239 4271 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1445 614 470 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 2283 748 Size: 1535 3989 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KpnI G_GTAC'C Cuts at: 2730 2849 2730 Size: 119 5405 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3702 Fragments arranged by size: 3702 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2107 2165 3482 3841 4000 518 Size: 648 941 58 1317 359 159 2042 Fragments arranged by size: 2042 1317 941 648 359 159 58 MspA1I CmG'CkG Cuts at: 1606 2561 2940 3617 4765 5010 1606 Size: 955 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 955 677 379 245 NcoI C'CATG_G Cuts at: 513 1065 2102 2645 513 Size: 552 1037 543 3392 Fragments arranged by size: 3392 1037 552 543 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2179 2882 4427 1132 Size: 97 950 703 1545 2229 Fragments arranged by size: 2229 1545 950 703 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 2134 Size: 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2624 2656 2624 Size: 32 5492 PstI C_TGCA'G Cuts at: 830 2876 830 Size: 2046 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 PvuII CAG'CTG Cuts at: 2561 2561 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SfcI C'TryA_G Cuts at: 826 1045 2872 4007 4688 4879 826 Size: 219 1827 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 3628 4529 4791 820 Size: 197 926 366 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 366 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2179 2882 1132 Size: 1047 703 3774 Fragments arranged by size: 3774 1047 703 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2102 2645 2963 513 Size: 552 1037 543 318 3074 Fragments arranged by size: 3074 1037 552 543 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3551 3568 3721 3906 4351 325 Size: 1255 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3770 5482 96 Size: 274 80 33 51 1443 1793 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2139 2171 5484 5516 2139 Size: 32 3313 32 2147 Fragments arranged by size: 3313 2147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2362 2167 Size: 195 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI