(Linear) (Six-base) MAPSORT of: KIEE1554.seq Check: 1188 from: 1 to: 819 KIEE1554.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. Acc65I G'GTAC_C Cuts at: 0 721 819 Size: 721 98 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 421 701 765 819 Size: 64 357 280 64 54 Fragments arranged by size: 357 280 64 64 54 AlwNI CAG_nnn'CTG Cuts at: 0 381 819 Size: 381 438 AseI AT'TA_AT Cuts at: 0 545 819 Size: 545 274 AvaI C'yCGr_G Cuts at: 0 477 819 Size: 477 342 BanI G'GyrC_C Cuts at: 0 104 721 819 Size: 104 617 98 Fragments arranged by size: 617 104 98 BanII G_rGCy'C Cuts at: 0 484 819 Size: 484 335 BbsI GAAGACnn'nnnn_ Cuts at: 0 70 819 Size: 70 749 BglII A'GATC_T Cuts at: 0 251 819 Size: 251 568 BmrI ACTGGGnnnn_n' Cuts at: 0 501 819 Size: 501 318 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 819 Size: 262 557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 819 Size: 289 530 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 758 819 Size: 758 61 BseYI C'CCAG_C Cuts at: 0 165 631 819 Size: 165 466 188 Fragments arranged by size: 466 188 165 BsiHKAI G_wGCw'C Cuts at: 0 816 819 Size: 816 3 Bsp1286I G_dGCh'C Cuts at: 0 484 816 819 Size: 484 332 3 Fragments arranged by size: 484 332 3 BstXI CCAn_nnnn'nTGG Cuts at: 0 647 819 Size: 647 172 BstYI r'GATC_y Cuts at: 0 20 251 443 819 Size: 20 231 192 376 Fragments arranged by size: 376 231 192 20 BtgI C'CryG_G Cuts at: 0 97 640 819 Size: 97 543 179 Fragments arranged by size: 543 179 97 EarI CTCTTCn'nnn_ Cuts at: 0 397 819 Size: 397 422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 262 421 701 765 819 Size: 64 198 159 280 64 54 Fragments arranged by size: 280 198 159 64 64 54 EcoO109I rG'GnC_Cy Cuts at: 0 129 819 Size: 129 690 HaeII r_GCGC'y Cuts at: 0 698 819 Size: 698 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 37 69 683 715 819 Size: 37 32 614 32 104 Fragments arranged by size: 614 104 37 32 32 HindIII A'AGCT_T Cuts at: 0 278 819 Size: 278 541 KpnI G_GTAC'C Cuts at: 0 725 819 Size: 725 94 MslI CAynn'nnrTG Cuts at: 0 102 160 819 Size: 102 58 659 Fragments arranged by size: 659 102 58 MspA1I CmG'CkG Cuts at: 0 556 819 Size: 556 263 NcoI C'CATG_G Cuts at: 0 97 640 819 Size: 97 543 179 Fragments arranged by size: 543 179 97 NspI r_CATG'y Cuts at: 0 174 819 Size: 174 645 PpuMI rG'GwC_Cy Cuts at: 0 129 819 Size: 129 690 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 619 651 819 Size: 619 32 168 Fragments arranged by size: 619 168 32 PvuII CAG'CTG Cuts at: 0 556 819 Size: 556 263 SmlI C'TyrA_G Cuts at: 0 304 819 Size: 304 515 SphI G_CATG'C Cuts at: 0 174 819 Size: 174 645 StyI C'CwwG_G Cuts at: 0 97 640 819 Size: 97 543 179 Fragments arranged by size: 543 179 97 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 134 166 819 Size: 134 32 653 Fragments arranged by size: 653 134 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 162 357 819 Size: 162 195 462 Fragments arranged by size: 462 195 162 Enzymes that do cut: Acc65I AcuI AlwNI AseI AvaI BanI BanII BbsI BglII BmrI BpmI BpuEI BseRI BseYI BsiHKAI Bsp1286I BstXI BstYI BtgI EarI Eco57MI EcoO109I HaeII Hin4I HindIII KpnI MslI MspA1I NcoI NspI PpuMI PsrI PvuII SmlI SphI StyI TstI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI