################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BamHI BanII BbsI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI HincII HindII HindIII HinfI HpaI HphI KpnI KspI MaeI MaeIII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 370 GGCGCC G[GA]^CG[CT]C AspI 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 329 GGACC G^G[AT]CC BalI 341 TGGCCA TGG^CCA BbeI 370 GGCGCC GGCGC^C BbrPI 121 CACGTG CAC^GTG BbvCI 84 GCTGAGG CCTCAGC(-5/-2) BglI 97 GCCGTAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 121 CACGTG [CT]AC^GT[GA] BsaHI 370 GGCGCC G[GA]^CG[CT]C BsaI 223 GAGACC GGTCTC(1/5) BsmAI 223 GAGAC GTCTC(1/5) BsmFI 308 GGGAC GGGAC(10/14) BsrFI 13 GCCGGC [GA]^CCGG[CT] BstF5I 164 CATCC GGATG(2/0) BstXI 332 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 13 GCCGGC [GA]^CCGG[CT] DraIII 65 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 296 CTCTTC CTCTTC(1/4) FokI 164 CATCC GGATG(9/13) HaeII 370 GGCGCC [GA]GCGC^[CT] HapII 14 CCGG C^CGG Hin1I 370 GGCGCC G[GA]^CG[CT]C HpaII 14 CCGG C^CGG KasI 370 GGCGCC G^GCGCC Ksp632I 296 CTCTTC CTCTTC(1/4) MluNI 341 TGGCCA TGG^CCA MscI 341 TGGCCA TGG^CCA MslI 168 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 14 CCGG C^CGG MwoI 97 GCCGTAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 13 GCCGGC GCC^GGC NarI 370 GGCGCC GG^CGCC NgoMIV 13 GCCGGC G^CCGGC NlaIII 81 CATG CATG^ PflFI 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 121 CACGTG CAC^GTG PmlI 121 CACGTG CAC^GTG SfaNI 27 GATGC GCATC(5/9) SfoI 370 GGCGCC GGC^GCC SmlI 58 CTCAAG C^T[CT][GA]AG TaqI 47 TCGA T^CGA Tth111I 379 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 47 TCGA T^CGA XcmI 113 CCAAGCCGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 42 GAATTT [GA]^AATT[CT] 395 GAATTT AluI 53 AGCT AG^CT 338 AGCT ApoI 42 GAATTT [GA]^AATT[CT] 395 GAATTT AviII 29 TGCGCA TGC^GCA 176 TGCGCA BanI 16 GGCACC G^G[CT][GA]CC 370 GGCGCC BsaJI 170 CCACGG C^C[ACGT][ACGT]GG 227 CCGTGG BseRI 217 GAGGAG GAGGAG(10/8) 220 GAGGAG BssKI 20 CCTGG ^CC[ACGT]GG 135 CCAGG BstNI 20 CCTGG CC^[AT]GG 135 CCAGG BstYI 128 AGATCC [GA]^GATC[CT] 278 AGATCC Cfr13I 313 GGCCC G^G[ACGT]CC 329 GGACC DdeI 85 CTGAG C^T[ACGT]AG 185 CTGAG DsaI 170 CCACGG C^C[GA][CT]GG 227 CCGTGG EaeI 95 TGGCCG [CT]^GGCC[GA] 341 TGGCCA EcoRII 20 CCTGG ^CC[AT]GG 135 CCAGG FspI 29 TGCGCA TGC^GCA 176 TGCGCA HgaI 236 GACGC GACGC(5/10) 109 GCGTC MboII 24 GAAGA GAAGA(8/7) 297 TCTTC MflI 128 AGATCC [GA]^GATC[CT] 278 AGATCC MvaI 20 CCTGG CC^[AT]GG 135 CCAGG Sau96I 313 GGCCC G^G[ACGT]CC 329 GGACC ScrFI 20 CCTGG CC^[ACGT]GG 135 CCAGG Tsp509I 43 AATT ^AATT 396 AATT XhoII 128 AGATCC [GA]^GATC[CT] 278 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AlwI BbvI BsgI BsiYI BslI BsrI Cac8I CfoI DpnII Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MboI MnlI NdeII NlaIV Sau3AI TseI TspRI