################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NsiI PacI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 1231 CGCG CG^CG AciI 393 CCGC CCGC(-3/-1) AfaI 248 GTAC GT^AC Alw44I 1150 GTGCAC G^TGCAC AlwNI 681 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1150 GTGCAC G^TGCAC AspHI 1150 GTGCAC G[AT]GC[AT]^C AspI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1067 CTCGAG C^[CT]CG[GA]G BanII 712 GAGCCC G[GA]GC[CT]^C BcgI 1255 CGACTGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 553 CTAG C^TAG BsaWI 905 TCCGGT [AT]^CCGG[AT] BseRI 806 CTCCTC GAGGAG(10/8) BsiHKAI 1150 GTGCAC G[AT]GC[AT]^C BsmFI 138 GTCCC GGGAC(10/14) BsoBI 1067 CTCGAG C^[CT]CG[GA]G BspMI 912 ACCTGC ACCTGC(4/8) BstBI 1190 TTCGAA TT^CGAA BstEII 908 GGTCACC G^GT[ACGT]ACC BstPI 908 GGTCACC G^GT[ACGT]ACC BstUI 1231 CGCG CG^CG BstXI 743 CCAAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EarI 998 GAAGAG CTCTTC(1/4) EcoO65I 908 GGTCACC G^GT[ACGT]ACC EcoT14I 734 CCAAGG C^C[AT][AT]GG FauI 392 CCCGC CCCGC(4/6) HaeIII 1042 GGCC GG^CC HapII 906 CCGG C^CGG HgaI 369 GCGTC GACGC(5/10) HindIII 324 AAGCTT A^AGCTT HinfI 621 GATTC G^A[ACGT]TC HpaII 906 CCGG C^CGG Ksp632I 998 GAAGAG CTCTTC(1/4) MaeI 553 CTAG C^TAG MspA1I 99 CAGCTG C[AC]G^C[GT]G MspI 906 CCGG C^CGG MvnI 1231 CGCG CG^CG NruI 1230 TCGCGA TCG^CGA NspI 723 GCATGC [GA]CATG^[CT] NspV 1190 TTCGAA TT^CGAA PaeR7I 1067 CTCGAG C^TCGAG PflFI 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 99 CAGCTG CAG^CTG RsaI 248 GTAC GT^AC SfuI 1190 TTCGAA TT^CGAA SmlI 1067 CTCGAG C^T[CT][GA]AG SphI 723 GCATGC GCATG^C SspI 1225 AATATT AAT^ATT StyI 734 CCAAGG C^C[AT][AT]GG SwaI 843 ATTTAAAT ATTT^AAAT TfiI 621 GATTC G^A[AT]TC Tth111I 456 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1067 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 929 GAATTT [GA]^AATT[CT] 1005 AAATTT ApoI 929 GAATTT [GA]^AATT[CT] 1005 AAATTT Asp700I 568 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1100 GAAAACCTTC BbsI 371 GTCTTC GAAGAC(2/6) 1294 GTCTTC BbvI 98 GCAGC GCAGC(8/12) 720 GCTGC BpuAI 371 GTCTTC GAAGAC(2/6) 1294 GTCTTC BsaJI 141 CCCTGG C^C[ACGT][ACGT]GG 734 CCAAGG BsrDI 729 CATTGC GCAATG(2/0) 916 GCAATG DraI 291 TTTAAA TTT^AAA 844 TTTAAA DraII 34 GGGTCCT [GA]G^G[ACGT]CC[CT] 737 AGGACCC EcoO109I 34 GGGTCCT [GA]G^G[ACGT]CC[CT] 737 AGGACCC Fnu4HI 98 GCAGC GC^[ACGT]GC 720 GCTGC ItaI 98 GCAGC GC^[ACGT]GC 720 GCTGC MaeII 942 ACGT A^CGT 1154 ACGT MslI 85 CATAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1271 CATTTCAGTG PpuMI 34 GGGTCCT [GA]G^G[AT]CC[CT] 737 AGGACCC TseI 98 GCAGC G^C[AT]GC 720 GCTGC XmnI 568 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1100 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AluI AlwI AvaII BmyI BpmI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FokI HphI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII