(Circular) (Six-base) MAPSORT of: pFN21AE6425.seq Check: 9783 from: 1 to: 6034 pFN21AE6425 6034 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2926 2926 Size: 6034 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3377 1937 Size: 1440 4594 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 4097 5481 5749 1632 Size: 1011 1454 1384 268 1917 Fragments arranged by size: 1917 1454 1384 1011 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2751 1184 Size: 1567 4467 AlwNI CAG_nnn'CTG Cuts at: 2691 3777 5349 2691 Size: 1086 1572 3376 Fragments arranged by size: 3376 1572 1086 ApaLI G'TGCA_C Cuts at: 3155 4091 5247 3155 Size: 936 1156 3942 Fragments arranged by size: 3942 1156 936 ApoI r'AATT_y Cuts at: 1493 2934 3010 3343 3569 3721 5830 1493 Size: 1441 76 333 226 152 2109 1697 Fragments arranged by size: 2109 1697 1441 333 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 3072 3359 1870 Size: 73 1129 287 4545 Fragments arranged by size: 4545 1129 287 73 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3355 4810 618 Size: 325 217 627 1568 1455 1842 Fragments arranged by size: 1842 1568 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2721 3353 1391 Size: 1330 632 4072 Fragments arranged by size: 4072 1330 632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2369 3292 928 Size: 971 470 923 3670 Fragments arranged by size: 3670 971 923 470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3249 3283 4225 4259 1903 Size: 34 1312 34 942 34 3678 Fragments arranged by size: 3678 1312 942 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4645 136 Size: 107 122 71 631 605 2973 1525 Fragments arranged by size: 2973 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2765 3159 4095 5251 1790 Size: 975 394 936 1156 2573 Fragments arranged by size: 2573 1156 975 936 394 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3272 3765 4723 Size: 416 53 83 399 24 1855 493 958 Cuts at: 4723 442 Size: 1753 Fragments arranged by size: 1855 1753 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3068 3068 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 2816 2949 4694 1239 Size: 1496 81 133 1745 2579 Fragments arranged by size: 2579 1745 1496 133 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 5024 5322 4159 Size: 865 298 4871 Fragments arranged by size: 4871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4697 882 Size: 617 3198 2219 Fragments arranged by size: 3198 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3825 493 Size: 711 2621 2702 Fragments arranged by size: 2702 2621 711 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2910 4467 4865 5139 5286 5697 2910 Size: 1557 398 274 147 411 3247 Fragments arranged by size: 3247 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 5065 Fragments arranged by size: 5065 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2802 1186 Size: 1616 4418 BseYI C'CCAG_C Cuts at: 2392 5237 5733 2392 Size: 2845 496 2693 Fragments arranged by size: 2845 2693 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3484 4245 5273 1540 Size: 275 186 1483 761 1028 2301 Fragments arranged by size: 2301 1483 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3159 4095 4180 5251 3159 Size: 936 85 1071 3942 Fragments arranged by size: 3942 1071 936 85 BsmI GAATG_Cn' Cuts at: 3545 3638 3545 Size: 93 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2721 2765 3159 3353 4095 4180 5251 Size: 399 931 44 394 194 742 85 1071 Cuts at: 5251 1391 Size: 2174 Fragments arranged by size: 2174 1071 931 742 399 394 194 85 44 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 3389 844 Size: 2082 463 3489 Fragments arranged by size: 3489 2082 463 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2733 2928 4529 4703 65 Size: 1183 1485 195 1601 174 1396 Fragments arranged by size: 1601 1485 1396 1183 195 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4678 4865 1700 Size: 110 2868 187 2869 Fragments arranged by size: 2869 2868 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 4094 5106 4094 Size: 1012 5022 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 3196 4838 3196 Size: 1642 4392 BstEII G'GTnAC_C Cuts at: 1184 2913 1184 Size: 1729 4305 BstXI CCAn_nnnn'nTGG Cuts at: 2755 2755 Size: 6034 BstYI r'GATC_y Cuts at: 1972 2055 2894 3030 3364 3731 4116 4133 5585 Size: 83 839 136 334 367 385 17 1452 Cuts at: 5585 5671 5683 6029 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 839 385 367 346 334 136 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5482 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4344 4364 3550 Size: 794 20 5220 Fragments arranged by size: 5220 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 2298 2851 3338 2298 Size: 553 487 4994 Fragments arranged by size: 4994 553 487 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3481 4370 5999 Size: 54 1425 50 275 18 1651 889 1629 Cuts at: 5999 8 Size: 43 Fragments arranged by size: 1651 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3481 1537 Size: 275 1669 4090 Fragments arranged by size: 4090 1669 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2998 3964 1353 Size: 1645 966 3423 Fragments arranged by size: 3423 1645 966 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4621 4995 5141 387 Size: 1064 138 3032 374 146 1280 Fragments arranged by size: 3032 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2643 2735 2816 2949 4097 4694 5481 Size: 393 1011 92 81 133 1148 597 787 Cuts at: 5481 5749 1239 Size: 268 1524 Fragments arranged by size: 1524 1148 1011 787 597 393 268 133 92 81 EcoO109I rG'GnC_Cy Cuts at: 2040 2743 2040 Size: 703 5331 EcoRI G'AATT_C Cuts at: 3343 3721 3343 Size: 378 5656 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2844 2844 Size: 6034 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 5181 1678 Size: 3503 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 4675 4707 4749 Size: 32 135 32 545 32 2526 32 42 Cuts at: 4749 4781 1373 Size: 32 2626 Fragments arranged by size: 2626 2526 545 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3378 3624 669 Size: 988 281 1440 246 3079 Fragments arranged by size: 3079 1440 988 281 246 HindIII A'AGCT_T Cuts at: 748 2329 748 Size: 1581 4453 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3291 5148 5332 1305 Size: 1986 1857 184 2007 Fragments arranged by size: 2007 1986 1857 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4212 Fragments arranged by size: 4212 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 3280 3992 4351 4510 518 Size: 648 928 1186 712 359 159 2042 Fragments arranged by size: 2042 1186 928 712 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 3450 4127 5275 5520 1606 Size: 500 1344 677 1148 245 2120 Fragments arranged by size: 2120 1344 1148 677 500 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5482 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NruI TCG'CGA Cuts at: 3237 3237 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2732 3392 4937 1132 Size: 97 1503 660 1545 2229 Fragments arranged by size: 2229 1545 1503 660 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6034 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6034 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 6034 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 2743 2040 Size: 703 5331 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 3059 3604 3059 Size: 545 5489 PstI C_TGCA'G Cuts at: 830 3386 830 Size: 2556 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2106 2106 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 3382 4517 5198 5389 826 Size: 219 2337 1135 681 191 1471 Fragments arranged by size: 2337 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3361 1872 Size: 1489 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3072 4138 5039 5301 820 Size: 197 926 1129 1066 901 262 1553 Fragments arranged by size: 1553 1129 1066 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 2732 3392 1132 Size: 1600 660 3774 Fragments arranged by size: 3774 1600 660 SspI AAT'ATT Cuts at: 5 52 3232 3958 5 Size: 47 3180 726 2081 Fragments arranged by size: 3180 2081 726 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2739 3473 513 Size: 552 1674 734 3074 Fragments arranged by size: 3074 1674 734 552 SwaI ATTT'AAAT Cuts at: 2851 2851 Size: 6034 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4061 4078 4231 4416 4861 325 Size: 1255 2481 17 153 185 445 1498 Fragments arranged by size: 2481 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4280 5992 96 Size: 274 80 33 51 1443 2303 1712 138 Fragments arranged by size: 2303 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2142 2174 2298 2330 5994 6026 2142 Size: 32 124 32 3664 32 2150 Fragments arranged by size: 3664 2150 124 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2464 1316 Size: 1148 4886 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 3072 1943 Size: 1129 4905 XmaI C'CCGG_G Cuts at: 1870 3359 1870 Size: 1489 4545 XmnI GAAnn'nnTTC Cuts at: 2577 3109 4163 2577 Size: 532 1054 4448 Fragments arranged by size: 4448 1054 532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI