(Linear) (Six-base) MAPSORT of: KIEE6425.seq Check: 7409 from: 1 to: 1329 KIEE6425.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 921 1329 Size: 921 408 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 638 1329 Size: 638 691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 746 1329 Size: 746 583 AlwNI CAG_nnn'CTG Cuts at: 0 686 1329 Size: 686 643 ApaLI G'TGCA_C Cuts at: 0 1150 1329 Size: 1150 179 ApoI r'AATT_y Cuts at: 0 929 1005 1329 Size: 929 76 324 Fragments arranged by size: 929 324 76 AvaI C'yCGr_G Cuts at: 0 1067 1329 Size: 1067 262 BanII G_rGCy'C Cuts at: 0 716 1329 Size: 716 613 BbsI GAAGACnn'nnnn_ Cuts at: 0 364 1287 1329 Size: 364 923 42 Fragments arranged by size: 923 364 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1244 1278 1329 Size: 1244 34 51 Fragments arranged by size: 1244 51 34 Bme1580I G_kGCm'C Cuts at: 0 760 1154 1329 Size: 760 394 175 Fragments arranged by size: 760 394 175 BmrI ACTGGGnnnn_n' Cuts at: 0 1267 1329 Size: 1267 62 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1063 1329 Size: 1063 266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 811 944 1329 Size: 730 81 133 385 Fragments arranged by size: 730 385 133 81 BsaWI w'CCGG_w Cuts at: 0 905 1329 Size: 905 424 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 112 142 1329 Size: 112 30 1187 Fragments arranged by size: 1187 112 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 797 1329 Size: 797 532 BseYI C'CCAG_C Cuts at: 0 387 1329 Size: 387 942 BsiHKAI G_wGCw'C Cuts at: 0 1154 1329 Size: 1154 175 Bsp1286I G_dGCh'C Cuts at: 0 716 760 1154 1329 Size: 716 44 394 175 Fragments arranged by size: 716 394 175 44 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 921 1329 Size: 921 408 BsrDI GCAATG_nn' Cuts at: 0 728 923 1329 Size: 728 195 406 Fragments arranged by size: 728 406 195 BstBI TT'CG_AA Cuts at: 0 1191 1329 Size: 1191 138 BstEII G'GTnAC_C Cuts at: 0 908 1329 Size: 908 421 BstXI CCAn_nnnn'nTGG Cuts at: 0 750 1329 Size: 750 579 BstYI r'GATC_y Cuts at: 0 50 889 1025 1329 Size: 50 839 136 304 Fragments arranged by size: 839 304 136 50 DraI TTT'AAA Cuts at: 0 293 846 1329 Size: 293 553 483 Fragments arranged by size: 553 483 293 EarI CTCTTCn'nnn_ Cuts at: 0 993 1329 Size: 993 336 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 638 730 811 944 1329 Size: 638 92 81 133 385 Fragments arranged by size: 638 385 133 92 81 EcoO109I rG'GnC_Cy Cuts at: 0 35 738 1329 Size: 35 703 591 Fragments arranged by size: 703 591 35 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 839 1329 Size: 839 490 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 112 144 1329 Size: 112 32 1185 Fragments arranged by size: 1185 112 32 HindIII A'AGCT_T Cuts at: 0 324 1329 Size: 324 1005 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1286 1329 Size: 1286 43 MslI CAynn'nnrTG Cuts at: 0 89 1275 1329 Size: 89 1186 54 Fragments arranged by size: 1186 89 54 MspA1I CmG'CkG Cuts at: 0 101 1329 Size: 101 1228 NruI TCG'CGA Cuts at: 0 1232 1329 Size: 1232 97 NspI r_CATG'y Cuts at: 0 727 1329 Size: 727 602 PfoI T'CCnGG_A Cuts at: 0 674 1329 Size: 674 655 PpuMI rG'GwC_Cy Cuts at: 0 35 738 1329 Size: 35 703 591 Fragments arranged by size: 703 591 35 PsiI TTA'TAA Cuts at: 0 1054 1329 Size: 1054 275 PvuII CAG'CTG Cuts at: 0 101 1329 Size: 101 1228 SmlI C'TyrA_G Cuts at: 0 1067 1329 Size: 1067 262 SphI G_CATG'C Cuts at: 0 727 1329 Size: 727 602 SspI AAT'ATT Cuts at: 0 1227 1329 Size: 1227 102 StyI C'CwwG_G Cuts at: 0 734 1329 Size: 734 595 SwaI ATTT'AAAT Cuts at: 0 846 1329 Size: 846 483 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 137 169 293 325 1329 Size: 137 32 124 32 1004 Fragments arranged by size: 1004 137 124 32 32 Tth111I GACn'n_nGTC Cuts at: 0 459 1329 Size: 459 870 XhoI C'TCGA_G Cuts at: 0 1067 1329 Size: 1067 262 XmnI GAAnn'nnTTC Cuts at: 0 572 1104 1329 Size: 572 532 225 Fragments arranged by size: 572 532 225 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaLI ApoI AvaI BanII BbsI BcgI Bme1580I BmrI BplI BpmI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BstBI BstEII BstXI BstYI DraI EarI Eco57MI EcoO109I FalI Hin4I HindIII MmeI MslI MspA1I NruI NspI PfoI PpuMI PsiI PvuII SmlI SphI SspI StyI SwaI TstI Tth111I XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI TaqII TatI XbaI XcmI XmaI ZraI