Restriction map for
pFN21AE6423
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BciVI
BclI BfaI BfrI BlnI BsaBI
BsaI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstXI Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
MaeI MaeIII MamI MfeI MluI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 87 GGCGTC G[GA]^CG[CT]C
AocI 1305 CCTGAGG CC^T[ACGT]AGG
ApaI 1031 GGGCCC GGGCC^C
BalI 215 TGGCCA TGG^CCA
BamHI 78 GGATCC G^GATCC
BanI 384 GGTGCC G^G[CT][GA]CC
BbrPI 837 CACGTG CAC^GTG
BbsI 343 GTCTTC GAAGAC(2/6)
BbvCI 52 GCTGAGG CCTCAGC(-5/-2)
BglI 895 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1317 GCTCAGC GC^T[ACGT]AGC
BpmI 1114 CTGGAG CTGGAG(16/14)
Bpu1102I 1317 GCTCAGC GC^T[ACGT]AGC
BpuAI 343 GTCTTC GAAGAC(2/6)
BsaAI 837 CACGTG [CT]AC^GT[GA]
BsaHI 87 GGCGTC G[GA]^CG[CT]C
BsmAI 1198 GAGAC GTCTC(1/5)
BsmBI 1198 GAGACG CGTCTC(1/5)
Bst1107I 316 GTATAC GTA^TAC
BstAPI 204 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 316 GTATAC GTA^TAC
Bsu36I 1305 CCTGAGG CC^T[ACGT]AGG
CelII 1317 GCTCAGC GC^T[ACGT]AGC
DrdI 334 GACTTCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1305 CCTGAGG CC^T[ACGT]AGG
Hin1I 87 GGCGTC G[GA]^CG[CT]C
HindIII 700 AAGCTT A^AGCTT
HphI 1229 TCACC GGTGA(8/7)
KspI 29 CCGCGG CCGC^GG
MluNI 215 TGGCCA TGG^CCA
MscI 215 TGGCCA TGG^CCA
MseI 556 TTAA T^TAA
NruI 705 TCGCGA TCG^CGA
NspI 273 GCATGT [GA]CATG^[CT]
PmaCI 837 CACGTG CAC^GTG
PmlI 837 CACGTG CAC^GTG
SacI 238 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SapI 516 GCTCTTC GCTCTTC(1/4)
SbfI 201 CCTGCAGG CCTGCA^GG
SexAI 470 ACCTGGT A^CC[AT]GGT
SmlI 511 CTCAAG C^T[CT][GA]AG
Sse8387I 201 CCTGCAGG CCTGCA^GG
StuI 867 AGGCCT AGG^CCT
TfiI 1146 GATTC G^A[AT]TC
Tru9I 556 TTAA T^TAA
TspRI 571 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 401 CCAAGAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 316 GTATAC GT^[AC][GT]AC
1216 GTCTAC
AccIII 246 TCCGGA T^CCGGA
674 TCCGGA
AcsI 586 GAATTC [GA]^AATT[CT]
880 GAATTC
AlwNI 205 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
856 CAGGCTCTG
Aor51HI 299 AGCGCT AGC^GCT
458 AGCGCT
ApoI 586 GAATTC [GA]^AATT[CT]
880 GAATTC
AspHI 238 GAGCTC G[AT]GC[AT]^C
1362 GTGCTC
BcnI 84 CCCGG CC^[GC]GG
663 CCGGG
BglII 93 AGATCT A^GATCT
753 AGATCT
BsaWI 246 TCCGGA [AT]^CCGG[AT]
674 TCCGGA
BseAI 246 TCCGGA T^CCGGA
674 TCCGGA
BseRI 70 GAGGAG GAGGAG(10/8)
444 GAGGAG
BsiHKAI 238 GAGCTC G[AT]GC[AT]^C
1362 GTGCTC
BsmI 158 GAATGC GAATGC(1/-1)
407 GAATGC
BspEI 246 TCCGGA T^CCGGA
674 TCCGGA
BsrI 4 ACTGG ACTGG(1/-1)
152 CCAGT
DsaI 29 CCGCGG C^C[GA][CT]GG
1103 CCGTGG
EaeI 6 TGGCCG [CT]^GGCC[GA]
215 TGGCCA
Eco47III 299 AGCGCT AGC^GCT
458 AGCGCT
EcoNI 849 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1188 CCTGCACCAGG
EcoRI 586 GAATTC G^AATTC
880 GAATTC
EcoT14I 617 CCAAGG C^C[AT][AT]GG
1269 CCAAGG
HincII 466 GTCAAC GT[CT]^[GA]AC
1255 GTCAAC
HindII 466 GTCAAC GT[CT]^[GA]AC
1255 GTCAAC
MroI 246 TCCGGA T^CCGGA
674 TCCGGA
NciI 84 CCCGG CC^[GC]GG
663 CCGGG
NlaIII 274 CATG CATG^
1000 CATG
StyI 617 CCAAGG C^C[AT][AT]GG
1269 CCAAGG
Tsp509I 587 AATT ^AATT
881 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AvaII BanII BbvI BcgI
BmyI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrBI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TthHB8I XhoII XmnI