(Circular) (Six-base) MAPSORT of: pFN21AE6423.seq Check: 2588 from: 1 to: 6100 pFN21AE6423 6100 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2227 2227 Size: 6100 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 3222 3443 1937 Size: 385 900 221 4594 Fragments arranged by size: 4594 900 385 221 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2480 2518 2876 4163 5547 5815 1632 Size: 662 186 38 358 1287 1384 268 1917 Fragments arranged by size: 1917 1384 1287 662 358 268 186 38 AfeI AGC'GCT Cuts at: 2306 2465 2306 Size: 159 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3164 3196 3164 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 2215 2866 3843 5415 2215 Size: 651 977 1572 2900 Fragments arranged by size: 2900 1572 977 651 ApaI G_GGCC'C Cuts at: 3040 3040 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 2591 2885 3409 3635 3787 5896 1493 Size: 1098 294 524 226 152 2109 1697 Fragments arranged by size: 2109 1697 1098 524 294 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 3425 1870 Size: 73 1482 4545 Fragments arranged by size: 4545 1482 73 BamHI G'GATC_C Cuts at: 2083 3430 3797 2083 Size: 1347 367 4386 Fragments arranged by size: 4386 1347 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 3421 4876 618 Size: 325 217 627 602 1032 1455 1842 Fragments arranged by size: 1842 1455 1032 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 2127 2247 2458 3040 3419 1391 Size: 736 120 211 582 379 4072 Fragments arranged by size: 4072 736 582 379 211 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2341 928 Size: 971 442 4687 Fragments arranged by size: 4687 971 442 BbvCI CC'TCA_GC Cuts at: 2058 2058 Size: 6100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2794 2828 2891 2925 3148 3182 4291 Size: 34 857 34 63 34 223 34 1109 Cuts at: 4291 4325 1903 Size: 34 3678 Fragments arranged by size: 3678 1109 857 223 63 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2906 4711 136 Size: 107 122 71 631 605 1234 1805 1525 Fragments arranged by size: 1805 1525 1234 631 605 122 107 71 BglII A'GATC_T Cuts at: 2098 2758 6095 2098 Size: 660 3337 2103 Fragments arranged by size: 3337 2103 660 BlpI GC'TnA_GC Cuts at: 3323 3517 3323 Size: 194 5906 Bme1580I G_kGCm'C Cuts at: 1790 3040 3282 4161 5317 1790 Size: 1250 242 879 1156 2573 Fragments arranged by size: 2573 1250 1156 879 242 BmgBI CAC'GTC Cuts at: 1318 2941 3220 1318 Size: 1623 279 4198 Fragments arranged by size: 4198 1623 279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2151 3831 4789 Size: 416 53 83 399 24 734 1680 958 Cuts at: 4789 442 Size: 1753 Fragments arranged by size: 1753 1680 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3140 4760 1239 Size: 1901 1620 2579 Fragments arranged by size: 2579 1901 1620 Bpu10I CC'TnA_GC Cuts at: 2058 2058 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 4225 5090 5388 2501 Size: 1724 865 298 3213 Fragments arranged by size: 3213 1724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2844 3891 493 Size: 711 1640 1047 2702 Fragments arranged by size: 2702 1640 1047 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2093 3907 4289 4924 Size: 53 83 186 936 560 1814 382 635 Cuts at: 4924 275 Size: 1451 Fragments arranged by size: 1814 1451 936 635 560 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2251 2679 4533 4931 5205 5352 5763 2251 Size: 428 1854 398 274 147 411 2588 Fragments arranged by size: 2588 1854 428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 1178 Size: 30 165 30 1761 30 4084 Fragments arranged by size: 4084 1761 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2464 1186 Size: 904 374 4822 Fragments arranged by size: 4822 904 374 BseYI C'CCAG_C Cuts at: 2149 2416 2635 5303 5799 2149 Size: 267 219 2668 496 2450 Fragments arranged by size: 2668 2450 496 267 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2255 2351 2570 2895 3026 3179 Size: 573 423 96 96 219 325 131 153 Cuts at: 3179 1163 Size: 4084 Fragments arranged by size: 4084 573 423 325 219 153 131 96 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 3371 4161 4246 5317 2247 Size: 1124 790 85 1071 3030 Fragments arranged by size: 3030 1124 1071 790 85 BsmI GAATG_Cn' Cuts at: 2169 2418 3611 3704 2169 Size: 249 1193 93 4565 Fragments arranged by size: 4565 1193 249 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3197 3197 Size: 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2247 2458 3040 3282 3371 3419 Size: 399 337 120 211 582 242 89 48 Cuts at: 3419 4161 4246 5317 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 399 337 242 211 120 89 85 48 BspEI T'CCGG_A Cuts at: 2251 2679 5763 2251 Size: 428 3084 2588 Fragments arranged by size: 3084 2588 428 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 2227 3234 3455 844 Size: 1290 93 1007 221 3489 Fragments arranged by size: 3489 1290 1007 221 93 BsrBI CCG'CTC Cuts at: 1394 1823 2725 2828 3001 3987 1394 Size: 429 902 103 173 986 3507 Fragments arranged by size: 3507 986 902 429 173 103 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4744 4931 1700 Size: 110 2934 187 2869 Fragments arranged by size: 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2215 794 Size: 1421 4679 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2083 2098 2758 2968 3270 3430 3797 4182 Size: 111 15 660 210 302 160 367 385 Cuts at: 4182 4199 5651 5737 5749 6095 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 660 385 367 346 302 210 160 111 86 17 15 12 BstZ17I GTA'TAC Cuts at: 2323 2323 Size: 6100 Bsu36I CC'TnA_GG Cuts at: 3311 3311 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2034 3108 513 Size: 552 969 1074 3505 Fragments arranged by size: 3505 1074 969 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2033 487 Size: 683 56 807 4554 Fragments arranged by size: 4554 807 683 56 BtsI GCAGTG_nn' Cuts at: 3616 4410 4430 3616 Size: 794 20 5286 Fragments arranged by size: 5286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2345 5107 809 Size: 507 1029 2762 1802 Fragments arranged by size: 2762 1802 1029 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2220 3547 Size: 54 1425 50 275 18 181 209 1327 Cuts at: 3547 4436 6065 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1327 889 275 209 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2528 2925 2966 3056 4030 1353 Size: 920 255 397 41 90 974 3423 Fragments arranged by size: 3423 974 920 397 255 90 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 2096 4687 5061 5207 387 Size: 1064 138 489 18 2591 374 146 1280 Fragments arranged by size: 2591 1280 1064 489 374 146 138 18 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2480 2518 2876 3140 4163 4760 Size: 393 662 186 38 358 264 1023 597 Cuts at: 4760 5547 5815 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1023 787 662 597 393 358 268 264 186 38 EcoNI CCTnn'n_nnAGG Cuts at: 2858 3197 2858 Size: 339 5761 EcoO109I rG'GnC_Cy Cuts at: 2146 2263 2850 2989 3037 2146 Size: 117 587 139 48 5209 Fragments arranged by size: 5209 587 139 117 48 EcoRI G'AATT_C Cuts at: 2591 2885 3409 3787 2591 Size: 294 524 378 4904 Fragments arranged by size: 4904 524 378 294 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2284 2284 Size: 6100 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2308 2467 3081 5247 1678 Size: 630 159 614 2166 2531 Fragments arranged by size: 2531 2166 630 614 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3089 3121 4741 4773 4815 Size: 32 135 32 1517 32 1620 32 42 Cuts at: 4815 4847 1373 Size: 32 2626 Fragments arranged by size: 2626 1620 1517 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 3262 3444 3690 669 Size: 988 281 535 789 182 246 3079 Fragments arranged by size: 3079 988 789 535 281 246 182 HindIII A'AGCT_T Cuts at: 748 2705 748 Size: 1957 4143 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5214 5398 1305 Size: 3909 184 2007 Fragments arranged by size: 3909 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 10 Size: 54 1425 343 390 3888 Fragments arranged by size: 3888 1425 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2036 2057 2188 2538 2639 2812 3034 Size: 423 7 21 131 350 101 173 222 Cuts at: 3034 3118 3327 3516 4193 5341 5586 1606 Size: 84 209 189 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 423 350 245 222 209 189 173 131 101 84 21 7 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5548 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NruI TCG'CGA Cuts at: 2712 2712 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2282 3458 5003 1132 Size: 97 1053 1176 1545 2229 Fragments arranged by size: 2229 1545 1176 1053 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PmlI CAC'GTG Cuts at: 2844 2844 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3164 3196 4169 4201 5682 5714 3164 Size: 32 973 32 1481 32 3550 Fragments arranged by size: 3550 1481 973 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2850 2989 2146 Size: 704 139 5257 Fragments arranged by size: 5257 704 139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 3036 3036 Size: 6100 PstI C_TGCA'G Cuts at: 830 2044 2211 2979 3452 830 Size: 1214 167 768 473 3478 Fragments arranged by size: 3478 1214 768 473 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2057 2639 3118 3327 2057 Size: 582 479 209 4830 Fragments arranged by size: 4830 582 479 209 SacI G_AGCT'C Cuts at: 2247 2247 Size: 6100 SacII CC_GC'GG Cuts at: 2037 2037 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SanDI GG'GwC_CC Cuts at: 2850 2850 Size: 6100 SapI GCTCTTCn'nnn_ Cuts at: 2528 2528 Size: 6100 SbfI CC_TGCA'GG Cuts at: 2211 3452 2211 Size: 1241 4859 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SexAI A'CCwGG_T Cuts at: 2475 2475 Size: 6100 SfcI C'TryA_G Cuts at: 826 1045 2040 2207 2975 3448 4583 5264 5455 Size: 219 995 167 768 473 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1135 995 768 681 473 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 4204 5105 5367 820 Size: 197 926 573 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2874 1517 Size: 1357 4743 StyI C'CwwG_G Cuts at: 513 1065 2622 3274 3539 513 Size: 552 1557 652 265 3074 Fragments arranged by size: 3074 1557 652 552 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 4127 4144 4297 4482 4927 325 Size: 1255 839 1708 17 153 185 445 1498 Fragments arranged by size: 1708 1498 1255 839 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3048 3186 4346 Size: 274 80 33 51 1443 1071 138 1160 Cuts at: 4346 6058 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1160 1071 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3374 3406 6060 6092 3374 Size: 32 2654 32 3382 Fragments arranged by size: 3382 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 2413 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 2465 2960 3176 4229 2465 Size: 495 216 1053 4336 Fragments arranged by size: 4336 1053 495 216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PspXI PsrI RsrII SfiI SfoI SrfI SwaI