(Linear) (Six-base) MAPSORT of: KIEE6423.seq Check: 4430 from: 1 to: 1395 KIEE6423.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 222 1395 Size: 222 1173 AccI GT'mk_AC Cuts at: 0 317 1217 1395 Size: 317 900 178 Fragments arranged by size: 900 317 178 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 475 513 871 1395 Size: 289 186 38 358 524 Fragments arranged by size: 524 358 289 186 38 AfeI AGC'GCT Cuts at: 0 301 460 1395 Size: 301 159 935 Fragments arranged by size: 935 301 159 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1395 Size: 1159 32 204 Fragments arranged by size: 1159 204 32 AlwNI CAG_nnn'CTG Cuts at: 0 210 861 1395 Size: 210 651 534 Fragments arranged by size: 651 534 210 ApaI G_GGCC'C Cuts at: 0 1035 1395 Size: 1035 360 ApoI r'AATT_y Cuts at: 0 586 880 1395 Size: 586 294 515 Fragments arranged by size: 586 515 294 BamHI G'GATC_C Cuts at: 0 78 1395 Size: 78 1317 BanI G'GyrC_C Cuts at: 0 384 1395 Size: 384 1011 BanII G_rGCy'C Cuts at: 0 122 242 453 1035 1395 Size: 122 120 211 582 360 Fragments arranged by size: 582 360 211 122 120 BbsI GAAGACnn'nnnn_ Cuts at: 0 336 1395 Size: 336 1059 BbvCI CC'TCA_GC Cuts at: 0 53 1395 Size: 53 1342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 789 823 886 920 1143 1177 1395 Size: 789 34 63 34 223 34 218 Fragments arranged by size: 789 223 218 63 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 901 1395 Size: 901 494 BglII A'GATC_T Cuts at: 0 93 753 1395 Size: 93 660 642 Fragments arranged by size: 660 642 93 BlpI GC'TnA_GC Cuts at: 0 1318 1395 Size: 1318 77 Bme1580I G_kGCm'C Cuts at: 0 1035 1277 1395 Size: 1035 242 118 Fragments arranged by size: 1035 242 118 BmgBI CAC'GTC Cuts at: 0 936 1215 1395 Size: 936 279 180 Fragments arranged by size: 936 279 180 BmrI ACTGGGnnnn_n' Cuts at: 0 146 1395 Size: 146 1249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1135 1395 Size: 1135 260 Bpu10I CC'TnA_GC Cuts at: 0 53 1395 Size: 53 1342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 1395 Size: 496 899 BsaAI yAC'GTr Cuts at: 0 839 1395 Size: 839 556 BsaHI Gr'CG_yC Cuts at: 0 88 1395 Size: 88 1307 BsaWI w'CCGG_w Cuts at: 0 246 674 1395 Size: 246 428 721 Fragments arranged by size: 721 428 246 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1159 1189 1395 Size: 1159 30 206 Fragments arranged by size: 1159 206 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 85 459 1395 Size: 85 374 936 Fragments arranged by size: 936 374 85 BseYI C'CCAG_C Cuts at: 0 144 411 630 1395 Size: 144 267 219 765 Fragments arranged by size: 765 267 219 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 250 346 565 890 1021 1174 1395 Size: 154 96 96 219 325 131 153 221 Fragments arranged by size: 325 221 219 154 153 131 96 96 BsiHKAI G_wGCw'C Cuts at: 0 242 1366 1395 Size: 242 1124 29 Fragments arranged by size: 1124 242 29 BsmI GAATG_Cn' Cuts at: 0 164 413 1395 Size: 164 249 982 Fragments arranged by size: 982 249 164 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1192 1395 Size: 1192 203 Bsp1286I G_dGCh'C Cuts at: 0 122 242 453 1035 1277 1366 1395 Size: 122 120 211 582 242 89 29 Fragments arranged by size: 582 242 211 122 120 89 29 BspEI T'CCGG_A Cuts at: 0 246 674 1395 Size: 246 428 721 Fragments arranged by size: 721 428 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 129 222 1229 1395 Size: 129 93 1007 166 Fragments arranged by size: 1007 166 129 93 BsrBI CCG'CTC Cuts at: 0 720 823 996 1395 Size: 720 103 173 399 Fragments arranged by size: 720 399 173 103 BstAPI GCAn_nnn'nTGC Cuts at: 0 210 1395 Size: 210 1185 BstYI r'GATC_y Cuts at: 0 78 93 753 963 1265 1395 Size: 78 15 660 210 302 130 Fragments arranged by size: 660 302 210 130 78 15 BstZ17I GTA'TAC Cuts at: 0 318 1395 Size: 318 1077 Bsu36I CC'TnA_GG Cuts at: 0 1306 1395 Size: 1306 89 BtgI C'CryG_G Cuts at: 0 29 1103 1395 Size: 29 1074 292 Fragments arranged by size: 1074 292 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 28 1395 Size: 28 1367 DrdI GACnn_nn'nnGTC Cuts at: 0 340 1395 Size: 340 1055 EaeI y'GGCC_r Cuts at: 0 6 215 1395 Size: 6 209 1180 Fragments arranged by size: 1180 209 6 EarI CTCTTCn'nnn_ Cuts at: 0 268 523 920 961 1051 1395 Size: 268 255 397 41 90 344 Fragments arranged by size: 397 344 268 255 90 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 73 91 1395 Size: 73 18 1304 Fragments arranged by size: 1304 73 18 EcoICRI GAG'CTC Cuts at: 0 240 1395 Size: 240 1155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 475 513 871 1135 1395 Size: 289 186 38 358 264 260 Fragments arranged by size: 358 289 264 260 186 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 853 1192 1395 Size: 853 339 203 Fragments arranged by size: 853 339 203 EcoO109I rG'GnC_Cy Cuts at: 0 141 258 845 984 1032 1395 Size: 141 117 587 139 48 363 Fragments arranged by size: 587 363 141 139 117 48 EcoRI G'AATT_C Cuts at: 0 586 880 1395 Size: 586 294 515 Fragments arranged by size: 586 515 294 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 279 1395 Size: 279 1116 HaeII r_GCGC'y Cuts at: 0 303 462 1076 1395 Size: 303 159 614 319 Fragments arranged by size: 614 319 303 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1084 1116 1395 Size: 1084 32 279 Fragments arranged by size: 1084 279 32 HincII GTy'rAC Cuts at: 0 468 1257 1395 Size: 468 789 138 Fragments arranged by size: 789 468 138 HindIII A'AGCT_T Cuts at: 0 700 1395 Size: 700 695 MscI TGG'CCA Cuts at: 0 217 1395 Size: 217 1178 MspA1I CmG'CkG Cuts at: 0 24 31 52 183 533 634 807 1029 Size: 24 7 21 131 350 101 173 222 Cuts at: 1029 1113 1322 1395 Size: 84 209 73 Fragments arranged by size: 350 222 209 173 131 101 84 73 24 21 7 NruI TCG'CGA Cuts at: 0 707 1395 Size: 707 688 NspI r_CATG'y Cuts at: 0 277 1395 Size: 277 1118 PfoI T'CCnGG_A Cuts at: 0 347 1395 Size: 347 1048 PmlI CAC'GTG Cuts at: 0 839 1395 Size: 839 556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1395 Size: 1159 32 204 Fragments arranged by size: 1159 204 32 PpuMI rG'GwC_Cy Cuts at: 0 141 845 984 1395 Size: 141 704 139 411 Fragments arranged by size: 704 411 141 139 PspOMI G'GGCC_C Cuts at: 0 1031 1395 Size: 1031 364 PstI C_TGCA'G Cuts at: 0 39 206 974 1395 Size: 39 167 768 421 Fragments arranged by size: 768 421 167 39 PvuII CAG'CTG Cuts at: 0 52 634 1113 1322 1395 Size: 52 582 479 209 73 Fragments arranged by size: 582 479 209 73 52 SacI G_AGCT'C Cuts at: 0 242 1395 Size: 242 1153 SacII CC_GC'GG Cuts at: 0 32 1395 Size: 32 1363 SanDI GG'GwC_CC Cuts at: 0 845 1395 Size: 845 550 SapI GCTCTTCn'nnn_ Cuts at: 0 523 1395 Size: 523 872 SbfI CC_TGCA'GG Cuts at: 0 206 1395 Size: 206 1189 SexAI A'CCwGG_T Cuts at: 0 470 1395 Size: 470 925 SfcI C'TryA_G Cuts at: 0 35 202 970 1395 Size: 35 167 768 425 Fragments arranged by size: 768 425 167 35 SmlI C'TyrA_G Cuts at: 0 511 1395 Size: 511 884 StuI AGG'CCT Cuts at: 0 869 1395 Size: 869 526 StyI C'CwwG_G Cuts at: 0 617 1269 1395 Size: 617 652 126 Fragments arranged by size: 652 617 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 414 1395 Size: 414 981 TatI w'GTAC_w Cuts at: 0 1043 1181 1395 Size: 1043 138 214 Fragments arranged by size: 1043 214 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1369 1395 Size: 1369 26 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 408 1395 Size: 408 987 XmnI GAAnn'nnTTC Cuts at: 0 460 955 1171 1395 Size: 460 495 216 224 Fragments arranged by size: 495 460 224 216 Enzymes that do cut: AarI AccI AcuI AfeI AloI AlwNI ApaI ApoI BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BstAPI BstYI BstZ17I Bsu36I BtgI BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MscI MspA1I NruI NspI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BciVI BclI BfrBI BmtI BplI BsaI BsaBI BsiEI BsiWI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BtsI ClaI CspCI DraI DraIII EagI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PflMI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI