Restriction map for
pFN21AB5700
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwI 295 GATCC GGATC(4/5)
Asp700I 757 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 730 GGTCC G^G[AT]CC
AvrII 726 CCTAGG C^CTAGG
BbsI 73 GAAGAC GAAGAC(2/6)
BclI 863 TGATCA T^GATCA
BcnI 26 CCGGG CC^[GC]GG
BglII 699 AGATCT A^GATCT
BlnI 726 CCTAGG C^CTAGG
BpuAI 73 GAAGAC GAAGAC(2/6)
BsaBI 1207 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 290 GTGCAG GTGCAG(16/14)
BsmI 316 GCATTC GAATGC(1/-1)
Bsp1407I 1007 TGTACA T^GTACA
BsrGI 1007 TGTACA T^GTACA
Cac8I 539 GCAAGC GC[ACGT]^[ACGT]GC
Cfr13I 730 GGTCC G^G[ACGT]CC
DdeI 20 CTCAG C^T[ACGT]AG
DraIII 182 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 726 CCTAGG C^C[AT][AT]GG
FauI 935 CCCGC CCCGC(4/6)
FbaI 863 TGATCA T^GATCA
HaeIII 24 GGCC GG^CC
HapII 26 CCGG C^CGG
HincII 158 GTCAAC GT[CT]^[GA]AC
HindII 158 GTCAAC GT[CT]^[GA]AC
HindIII 1106 AAGCTT A^AGCTT
HpaII 26 CCGG C^CGG
MaeII 171 ACGT A^CGT
MamI 1207 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 26 CCGG C^CGG
NciI 26 CCGGG CC^[GC]GG
NlaIV 1183 GGAGCC GG[ACGT]^[ACGT]CC
NspI 614 GCATGT [GA]CATG^[CT]
PleI 375 GACTC GAGTC(4/5)
Sau96I 730 GGTCC G^G[ACGT]CC
SpeI 858 ACTAGT A^CTAGT
SspBI 1007 TGTACA T^GTACA
StyI 726 CCTAGG C^C[AT][AT]GG
TaqI 71 TCGA T^CGA
TthHB8I 71 TCGA T^CGA
XbaI 753 TCTAGA T^CTAGA
XcmI 945 CCAGGGAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 757 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 338 GTAC GT^AC
1008 GTAC
BbvI 32 GCAGC GCAGC(8/12)
268 GCTGC
BsiYI 594 CCCTGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
595 CCTGGAGGGGG
BslI 594 CCCTGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
595 CCTGGAGGGGG
BstYI 294 AGATCC [GA]^GATC[CT]
699 AGATCT
EarI 670 GAAGAG CTCTTC(1/4)
1150 GAAGAG
Fnu4HI 32 GCAGC GC^[ACGT]GC
268 GCTGC
ItaI 32 GCAGC GC^[ACGT]GC
268 GCTGC
Ksp632I 670 GAAGAG CTCTTC(1/4)
1150 GAAGAG
MflI 294 AGATCC [GA]^GATC[CT]
699 AGATCT
RsaI 338 GTAC GT^AC
1008 GTAC
SfaNI 415 GATGC GCATC(5/9)
1192 GATGC
TseI 32 GCAGC G^C[AT]GC
268 GCTGC
Tsp45I 136 GTCAC ^GT[GC]AC
1142 GTGAC
XhoII 294 AGATCC [GA]^GATC[CT]
699 AGATCT
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BpmI BsaJI BsrI BssKI BstF5I
BstNI DpnII EcoRII FokI HinfI
HphI MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfcI TfiI
Tru9I Tsp509I TspRI