################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspHI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MluI MluNI MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 5 CGCG CG^CG AccIII 1544 TCCGGA T^CCGGA AflII 1731 CTTAAG C^TTAAG AlwNI 227 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 103 AGCGCT AGC^GCT AvaI 32 CCCGGG C^[CT]CG[GA]G BbrPI 1314 CACGTG CAC^GTG BfrI 1731 CTTAAG C^TTAAG BsaAI 1314 CACGTG [CT]AC^GT[GA] BsaWI 1544 TCCGGA [AT]^CCGG[AT] BseAI 1544 TCCGGA T^CCGGA BsoBI 32 CCCGGG C^[CT]CG[GA]G BspEI 1544 TCCGGA T^CCGGA BspLU11I 420 ACATGT A^CATGT BsrBI 1468 CCGCTC CCGCTC(-3/-3) BstUI 5 CGCG CG^CG BstXI 1568 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 115 GACTTCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 858 CCACGG C^C[GA][CT]GG EaeI 152 CGGCCA [CT]^GGCC[GA] Eco47III 103 AGCGCT AGC^GCT HaeII 103 AGCGCT [GA]GCGC^[CT] HincII 1231 GTTGAC GT[CT]^[GA]AC HindII 1231 GTTGAC GT[CT]^[GA]AC MfeI 2069 CAATTG C^AATTG MroI 1544 TCCGGA T^CCGGA MslI 780 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 2069 CAATTG C^AATTG MvnI 5 CGCG CG^CG NdeI 1244 CATATG CA^TATG NheI 1672 GCTAGC G^CTAGC NspI 420 ACATGT [GA]CATG^[CT] PflMI 2135 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1314 CACGTG CAC^GTG PmlI 1314 CACGTG CAC^GTG PstI 55 CTGCAG CTGCA^G SmaI 32 CCCGGG CCC^GGG Van91I 2135 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 32 CCCGGG C^CCGGG XmaI 32 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AfaI 594 GTAC GT^AC 2028 GTAC AflIII 420 ACATGT A^C[GA][CT]GT 1313 ACACGT Asp700I 940 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1885 GAAGAGATTC AvaII 855 GGACC G^G[AT]CC 1385 GGTCC BamHI 1223 GGATCC G^GATCC 1736 GGATCC BbsI 930 GAAGAC GAAGAC(2/6) 1828 GAAGAC BcnI 32 CCCGG CC^[GC]GG 33 CCGGG BfaI 1673 CTAG C^TAG 1942 CTAG BmyI 88 GTGCCC G[AGT]GC[ACT]^C 94 GTGCCC BpuAI 930 GAAGAC GAAGAC(2/6) 1828 GAAGAC BsaBI 898 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1045 GATAAAGATC BsmFI 470 GGGAC GGGAC(10/14) 1386 GTCCC BsmI 841 GAATGC GAATGC(1/-1) 587 GCATTC Bsp1286I 88 GTGCCC G[AGT]GC[ACT]^C 94 GTGCCC BspMI 1515 ACCTGC ACCTGC(4/8) 57 GCAGGT BsrDI 823 GCAATG GCAATG(2/0) 925 GCAATG BssSI 1337 CACGAG CACGAG(-5/-1) 540 CTCGTG CfoI 4 GCGC GCG^C 104 GCGC HaeIII 153 GGCC GG^CC 2089 GGCC HapII 33 CCGG C^CGG 1545 CCGG HhaI 4 GCGC GCG^C 104 GCGC HinP1I 4 GCGC G^CGC 104 GCGC HpaII 33 CCGG C^CGG 1545 CCGG MaeI 1673 CTAG C^TAG 1942 CTAG MamI 898 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1045 GATAAAGATC MspA1I 227 CAGCTG C[AC]G^C[GT]G 935 CAGCTG MspI 33 CCGG C^CGG 1545 CCGG NciI 32 CCCGG CC^[GC]GG 33 CCGGG PleI 307 GAGTC GAGTC(4/5) 1415 GAGTC PvuII 227 CAGCTG CAG^CTG 935 CAGCTG RsaI 594 GTAC GT^AC 2028 GTAC SexAI 137 ACCTGGT A^CC[AT]GGT 572 ACCAGGT SfcI 55 CTGCAG C^T[GA][CT]AG 949 CTACAG SmlI 1731 CTTAAG C^T[CT][GA]AG 2032 CTTGAG XmnI 940 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1885 GAAGAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BanI BbvI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII