(Circular) (Six-base) MAPSORT of: pFN21AE1510.seq Check: 4798 from: 1 to: 6850 pFN21AE1510 6850 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3529 3529 Size: 6850 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4660 278 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4193 1937 Size: 2256 4594 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 4977 5350 1563 Size: 3414 373 3063 Fragments arranged by size: 3414 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 2862 2964 3852 3948 4037 4913 6297 Size: 956 274 102 888 96 89 876 1384 Cuts at: 6297 6565 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 956 888 876 274 268 102 96 89 AfeI AGC'GCT Cuts at: 2110 2110 Size: 6850 AflII C'TTAA_G Cuts at: 820 1017 3736 820 Size: 197 2719 3934 Fragments arranged by size: 3934 2719 197 AflIII A'CryG_T Cuts at: 1203 2425 3318 5749 6726 1203 Size: 1222 893 2431 977 1327 Fragments arranged by size: 2431 1327 1222 977 893 AgeI A'CCGG_T Cuts at: 5681 5681 Size: 6850 AhdI GACnn_n'nnGTC Cuts at: 5579 5579 Size: 6850 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6850 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 5734 AlwNI CAG_nnn'CTG Cuts at: 2237 4593 6165 2237 Size: 2356 1572 2922 Fragments arranged by size: 2922 2356 1572 ApaLI G'TGCA_C Cuts at: 4907 6063 4907 Size: 1156 5694 ApoI r'AATT_y Cuts at: 1493 2523 2880 2948 2985 3114 3257 3601 3610 Size: 1030 357 68 37 129 143 344 9 Cuts at: 3610 3775 4159 4385 4537 6646 1493 Size: 165 384 226 152 2109 1697 Fragments arranged by size: 2109 1697 1030 384 357 344 226 165 152 143 129 68 37 9 AseI AT'TA_AT Cuts at: 160 1032 5404 160 Size: 872 4372 1606 Fragments arranged by size: 4372 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 2037 4175 1870 Size: 73 94 2138 4545 Fragments arranged by size: 4545 2138 94 73 BamHI G'GATC_C Cuts at: 3228 3741 4180 4547 3228 Size: 513 439 367 5531 Fragments arranged by size: 5531 513 439 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2516 3517 4171 5626 Size: 325 217 627 305 424 1001 654 1455 Cuts at: 5626 618 Size: 1842 Fragments arranged by size: 1842 1455 1001 654 627 424 325 305 217 BanII G_rGCy'C Cuts at: 1391 4169 1391 Size: 2778 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2942 3840 928 Size: 971 1043 898 3938 Fragments arranged by size: 3938 1043 971 898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5041 5075 1903 Size: 34 3104 34 3678 Fragments arranged by size: 3678 3104 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4742 5952 4742 Size: 1210 5640 BclI T'GATC_A Cuts at: 1548 1548 Size: 6850 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5461 136 Size: 107 122 71 631 605 3789 1525 Fragments arranged by size: 3789 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6845 6845 Size: 6850 BlpI GC'TnA_GC Cuts at: 4267 4267 Size: 6850 Bme1580I G_kGCm'C Cuts at: 1790 2097 2103 4911 6067 1790 Size: 307 6 2808 1156 2573 Fragments arranged by size: 2808 2573 1156 307 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6850 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4581 5539 442 Size: 416 53 83 399 24 3164 958 1753 Fragments arranged by size: 3164 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3681 1056 Size: 2625 4225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2105 2382 3064 5510 1239 Size: 850 16 277 682 2446 2579 Fragments arranged by size: 2579 2446 850 682 277 16 Bpu10I CC'TnA_GC Cuts at: 2468 2468 Size: 6850 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4058 4975 5840 6138 4058 Size: 917 865 298 4770 Fragments arranged by size: 4770 917 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5513 882 Size: 617 4014 2219 Fragments arranged by size: 4014 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3321 4641 493 Size: 711 2117 1320 2702 Fragments arranged by size: 2702 2117 1320 711 BsaBI GATnn'nnATC Cuts at: 2907 3054 6844 2907 Size: 147 3790 2913 Fragments arranged by size: 3790 2913 147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4657 5039 5674 275 Size: 53 83 186 936 3124 382 635 1451 Fragments arranged by size: 3124 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3549 5283 5681 5955 6102 6513 3549 Size: 1734 398 274 147 411 3886 Fragments arranged by size: 3886 1734 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6625 Fragments arranged by size: 6625 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3701 3704 3770 3779 1186 Size: 2515 3 66 9 4257 Fragments arranged by size: 4257 2515 66 9 3 BseYI C'CCAG_C Cuts at: 2472 3162 4131 6053 6549 2472 Size: 690 969 1922 496 2773 Fragments arranged by size: 2773 1922 969 690 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2174 2306 3431 1163 Size: 573 399 39 132 1125 4582 Fragments arranged by size: 4582 1125 573 399 132 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4300 5061 6089 1540 Size: 275 186 2299 761 1028 2301 Fragments arranged by size: 2301 2299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4911 4996 6067 4911 Size: 85 1071 5694 Fragments arranged by size: 5694 1071 85 BsmI GAATG_Cn' Cuts at: 2592 2852 4361 4454 2592 Size: 260 1509 93 4988 Fragments arranged by size: 4988 1509 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2103 4169 4911 4996 6067 1391 Size: 399 307 6 2066 742 85 1071 2174 Fragments arranged by size: 2174 2066 1071 742 399 307 85 6 BspEI T'CCGG_A Cuts at: 3549 6513 3549 Size: 2964 3886 BspHI T'CATG_A Cuts at: 4739 6469 4739 Size: 1730 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3529 4205 844 Size: 1209 1476 676 3489 Fragments arranged by size: 3489 1476 1209 676 BsrBI CCG'CTC Cuts at: 1394 1823 3475 4737 1394 Size: 429 1652 1262 3507 Fragments arranged by size: 3507 1652 1262 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2835 2937 5345 5519 65 Size: 1183 1587 102 2408 174 1396 Fragments arranged by size: 2408 1587 1396 1183 174 102 BsrFI r'CCGG_y Cuts at: 1700 1810 5494 5681 1700 Size: 110 3684 187 2869 Fragments arranged by size: 3684 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6850 BssSI C'ACGA_G Cuts at: 2545 3342 4910 5922 2545 Size: 797 1568 1012 3473 Fragments arranged by size: 3473 1568 1012 797 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6850 BstBI TT'CG_AA Cuts at: 5654 5654 Size: 6850 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6850 BstXI CCAn_nnnn'nTGG Cuts at: 3580 3580 Size: 6850 BstYI r'GATC_y Cuts at: 1972 3228 3414 3741 4180 4547 4932 4949 6401 Size: 1256 186 327 439 367 385 17 1452 Cuts at: 6401 6487 6499 6845 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1256 439 385 367 346 327 186 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2863 513 Size: 552 939 859 4500 Fragments arranged by size: 4500 939 859 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6111 Fragments arranged by size: 6111 683 56 BtsI GCAGTG_nn' Cuts at: 4366 5160 5180 4366 Size: 794 20 6036 Fragments arranged by size: 6036 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6815 DraI TTT'AAA Cuts at: 4154 4154 Size: 6850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 5857 809 Size: 507 810 3731 1802 Fragments arranged by size: 3731 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 4297 5186 Size: 54 1425 50 275 18 327 2140 889 Cuts at: 5186 6815 8 Size: 1629 43 Fragments arranged by size: 2140 1629 1425 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4297 1537 Size: 275 2485 4090 Fragments arranged by size: 4090 2485 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 3885 4051 4780 1353 Size: 669 1863 166 729 3423 Fragments arranged by size: 3423 1863 729 669 166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5437 5811 5957 387 Size: 1064 138 3848 374 146 1280 Fragments arranged by size: 3848 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2105 2382 2588 2862 2964 3064 Size: 393 457 16 277 206 274 102 100 Cuts at: 3064 3852 3948 4037 4913 5510 6297 6565 1239 Size: 788 96 89 876 597 787 268 1524 Fragments arranged by size: 1524 876 788 787 597 457 393 277 274 268 206 102 100 96 89 16 EcoRI G'AATT_C Cuts at: 3257 3610 3775 4159 4537 3257 Size: 353 165 384 378 5570 Fragments arranged by size: 5570 384 378 353 165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2483 2483 Size: 6850 FspI TGC'GCA Cuts at: 5356 5356 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 2112 5997 1678 Size: 434 3885 2531 Fragments arranged by size: 3885 2531 434 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3230 3262 3297 3329 5491 Size: 32 135 32 1658 32 35 32 2162 Cuts at: 5491 5523 5565 5597 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2162 1658 135 42 35 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3238 4194 4440 669 Size: 988 281 1300 956 246 3079 Fragments arranged by size: 3079 1300 988 956 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 4074 4449 4074 Size: 375 6475 MluI A'CGCG_T Cuts at: 6726 6726 Size: 6850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3363 5964 6148 1305 Size: 2058 2601 184 2007 Fragments arranged by size: 2601 2058 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5028 Fragments arranged by size: 5028 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 4808 5167 5326 518 Size: 648 1623 2019 359 159 2042 Fragments arranged by size: 2042 2019 1623 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 2942 4266 4943 6091 6336 1606 Size: 628 708 1324 677 1148 245 2120 Fragments arranged by size: 2120 1324 1148 708 677 628 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5359 Fragments arranged by size: 5359 939 552 NdeI CA'TA_TG Cuts at: 387 3250 387 Size: 2863 3987 NheI G'CTAG_C Cuts at: 1052 3677 1052 Size: 2625 4225 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NspI r_CATG'y Cuts at: 1132 1229 2429 4208 5753 1132 Size: 97 1200 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 1200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6850 PciI A'CATG_T Cuts at: 2425 5749 2425 Size: 3324 3526 PflMI CCAn_nnn'nTGG Cuts at: 1108 4146 1108 Size: 3038 3812 PfoI T'CCnGG_A Cuts at: 3898 3898 Size: 6850 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PmlI CAC'GTG Cuts at: 3321 3321 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4919 4951 6432 6464 4919 Size: 32 1481 32 5305 Fragments arranged by size: 5305 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6850 PsiI TTA'TAA Cuts at: 2666 4420 2666 Size: 1754 5096 PstI C_TGCA'G Cuts at: 830 2064 4202 830 Size: 1234 2138 3478 Fragments arranged by size: 3478 2138 1234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 2234 2942 2234 Size: 708 6142 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SbfI CC_TGCA'GG Cuts at: 4202 4202 Size: 6850 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SexAI A'CCwGG_T Cuts at: 2142 2577 2142 Size: 435 6415 SfcI C'TryA_G Cuts at: 826 1045 2060 2954 4198 5333 6014 6205 826 Size: 219 1015 894 1244 1135 681 191 1471 Fragments arranged by size: 1471 1244 1135 1015 894 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 2039 4177 1872 Size: 167 2138 4545 Fragments arranged by size: 4545 2138 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4037 4954 5855 6117 820 Size: 197 926 1793 301 917 901 262 1553 Fragments arranged by size: 1793 1553 926 917 901 301 262 197 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 4208 1132 Size: 3076 3774 SrfI GCCC'GGGC Cuts at: 2039 2039 Size: 6850 SspI AAT'ATT Cuts at: 5 52 4774 5 Size: 47 4722 2081 Fragments arranged by size: 4722 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6850 StyI C'CwwG_G Cuts at: 513 1065 2004 2512 3394 3427 3781 4289 513 Size: 552 939 508 882 33 354 508 3074 Fragments arranged by size: 3074 939 882 552 508 508 354 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2261 3378 3926 4120 4877 4894 5047 Size: 1255 681 1117 548 194 757 17 153 Cuts at: 5047 5232 5677 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1117 757 681 548 445 194 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5096 6808 96 Size: 274 80 33 51 1443 3119 1712 138 Fragments arranged by size: 3119 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2446 2478 3393 3425 6810 6842 2446 Size: 32 915 32 3385 32 2454 Fragments arranged by size: 3385 2454 915 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6850 XbaI T'CTAG_A Cuts at: 4186 4186 Size: 6850 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2585 3248 3768 2585 Size: 663 520 5667 Fragments arranged by size: 5667 663 520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6850 XmaI C'CCGG_G Cuts at: 1870 2037 4175 1870 Size: 167 2138 4545 Fragments arranged by size: 4545 2138 167 XmnI GAAnn'nnTTC Cuts at: 2949 3894 4979 2949 Size: 945 1085 4820 Fragments arranged by size: 4820 1085 945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4658 276 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SwaI