(Linear) (Six-base) MAPSORT of: KIEE1510.seq Check: 320 from: 1 to: 2145 KIEE1510.orf With 182 enzymes: SgfI * July 15, 2010 18:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1524 2145 Size: 1524 621 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 857 959 1847 1943 2032 2145 Size: 583 274 102 888 96 89 113 Fragments arranged by size: 888 583 274 113 102 96 89 AfeI AGC'GCT Cuts at: 0 105 2145 Size: 105 2040 AflII C'TTAA_G Cuts at: 0 1731 2145 Size: 1731 414 AflIII A'CryG_T Cuts at: 0 420 1313 2145 Size: 420 893 832 Fragments arranged by size: 893 832 420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 2145 Size: 295 1850 AlwNI CAG_nnn'CTG Cuts at: 0 232 2145 Size: 232 1913 ApoI r'AATT_y Cuts at: 0 518 875 943 980 1109 1252 1596 1605 Size: 518 357 68 37 129 143 344 9 Cuts at: 1605 1770 2145 Size: 165 375 Fragments arranged by size: 518 375 357 344 165 143 129 68 37 9 AvaI C'yCGr_G Cuts at: 0 32 2145 Size: 32 2113 BamHI G'GATC_C Cuts at: 0 1223 1736 2145 Size: 1223 513 409 Fragments arranged by size: 1223 513 409 BanI G'GyrC_C Cuts at: 0 87 511 1512 2145 Size: 87 424 1001 633 Fragments arranged by size: 1001 633 424 87 BbsI GAAGACnn'nnnn_ Cuts at: 0 937 1835 2145 Size: 937 898 310 Fragments arranged by size: 937 898 310 Bme1580I G_kGCm'C Cuts at: 0 92 98 2145 Size: 92 6 2047 Fragments arranged by size: 2047 92 6 BmtI G_CTAG'C Cuts at: 0 1676 2145 Size: 1676 469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 100 377 1059 2145 Size: 84 16 277 682 1086 Fragments arranged by size: 1086 682 277 84 16 Bpu10I CC'TnA_GC Cuts at: 0 463 2145 Size: 463 1682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2053 2145 Size: 2053 92 BsaAI yAC'GTr Cuts at: 0 1316 2145 Size: 1316 829 BsaBI GATnn'nnATC Cuts at: 0 902 1049 2145 Size: 902 147 1096 Fragments arranged by size: 1096 902 147 BsaWI w'CCGG_w Cuts at: 0 1544 2145 Size: 1544 601 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1696 1699 1765 1774 2145 Size: 1696 3 66 9 371 Fragments arranged by size: 1696 371 66 9 3 BseYI C'CCAG_C Cuts at: 0 467 1157 2126 2145 Size: 467 690 969 19 Fragments arranged by size: 969 690 467 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 169 301 1426 2145 Size: 130 39 132 1125 719 Fragments arranged by size: 1125 719 132 130 39 BsmI GAATG_Cn' Cuts at: 0 587 847 2145 Size: 587 260 1298 Fragments arranged by size: 1298 587 260 Bsp1286I G_dGCh'C Cuts at: 0 92 98 2145 Size: 92 6 2047 Fragments arranged by size: 2047 92 6 BspEI T'CCGG_A Cuts at: 0 1544 2145 Size: 1544 601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 1524 2145 Size: 48 1476 621 Fragments arranged by size: 1476 621 48 BsrBI CCG'CTC Cuts at: 0 1470 2145 Size: 1470 675 BsrDI GCAATG_nn' Cuts at: 0 830 932 2145 Size: 830 102 1213 Fragments arranged by size: 1213 830 102 BssSI C'ACGA_G Cuts at: 0 540 1337 2145 Size: 540 797 808 Fragments arranged by size: 808 797 540 BstXI CCAn_nnnn'nTGG Cuts at: 0 1575 2145 Size: 1575 570 BstYI r'GATC_y Cuts at: 0 1223 1409 1736 2145 Size: 1223 186 327 409 Fragments arranged by size: 1223 409 327 186 BtgI C'CryG_G Cuts at: 0 858 2145 Size: 858 1287 DrdI GACnn_nn'nnGTC Cuts at: 0 121 2145 Size: 121 2024 EaeI y'GGCC_r Cuts at: 0 152 2145 Size: 152 1993 EarI CTCTTCn'nnn_ Cuts at: 0 17 1880 2046 2145 Size: 17 1863 166 99 Fragments arranged by size: 1863 166 99 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 100 377 583 857 959 1059 1847 Size: 84 16 277 206 274 102 100 788 Cuts at: 1847 1943 2032 2145 Size: 96 89 113 Fragments arranged by size: 788 277 274 206 113 102 100 96 89 84 16 EcoRI G'AATT_C Cuts at: 0 1252 1605 1770 2145 Size: 1252 353 165 375 Fragments arranged by size: 1252 375 353 165 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 478 2145 Size: 478 1667 HaeII r_GCGC'y Cuts at: 0 107 2145 Size: 107 2038 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1225 1257 1292 1324 2145 Size: 1225 32 35 32 821 Fragments arranged by size: 1225 821 35 32 32 HincII GTy'rAC Cuts at: 0 1233 2145 Size: 1233 912 MfeI C'AATT_G Cuts at: 0 2069 2145 Size: 2069 76 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1358 2145 Size: 1358 787 MslI CAynn'nnrTG Cuts at: 0 784 2145 Size: 784 1361 MspA1I CmG'CkG Cuts at: 0 229 937 2145 Size: 229 708 1208 Fragments arranged by size: 1208 708 229 NdeI CA'TA_TG Cuts at: 0 1245 2145 Size: 1245 900 NheI G'CTAG_C Cuts at: 0 1672 2145 Size: 1672 473 NspI r_CATG'y Cuts at: 0 424 2145 Size: 424 1721 PciI A'CATG_T Cuts at: 0 420 2145 Size: 420 1725 PflMI CCAn_nnn'nTGG Cuts at: 0 2141 2145 Size: 2141 4 PfoI T'CCnGG_A Cuts at: 0 1893 2145 Size: 1893 252 PmlI CAC'GTG Cuts at: 0 1316 2145 Size: 1316 829 PsiI TTA'TAA Cuts at: 0 661 2145 Size: 661 1484 PstI C_TGCA'G Cuts at: 0 59 2145 Size: 59 2086 PvuII CAG'CTG Cuts at: 0 229 937 2145 Size: 229 708 1208 Fragments arranged by size: 1208 708 229 SexAI A'CCwGG_T Cuts at: 0 137 572 2145 Size: 137 435 1573 Fragments arranged by size: 1573 435 137 SfcI C'TryA_G Cuts at: 0 55 949 2145 Size: 55 894 1196 Fragments arranged by size: 1196 894 55 SmaI CCC'GGG Cuts at: 0 34 2145 Size: 34 2111 SmlI C'TyrA_G Cuts at: 0 1731 2032 2145 Size: 1731 301 113 Fragments arranged by size: 1731 301 113 SrfI GCCC'GGGC Cuts at: 0 34 2145 Size: 34 2111 StyI C'CwwG_G Cuts at: 0 507 1389 1422 1776 2145 Size: 507 882 33 354 369 Fragments arranged by size: 882 507 369 354 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 256 1373 1921 2115 2145 Size: 256 1117 548 194 30 Fragments arranged by size: 1117 548 256 194 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 441 473 1388 1420 2145 Size: 441 32 915 32 725 Fragments arranged by size: 915 725 441 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 580 1243 1763 2145 Size: 580 663 520 382 Fragments arranged by size: 663 580 520 382 XmaI C'CCGG_G Cuts at: 0 32 2145 Size: 32 2113 XmnI GAAnn'nnTTC Cuts at: 0 944 1889 2145 Size: 944 945 256 Fragments arranged by size: 945 944 256 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AlfI AlwNI ApoI AvaI BamHI BanI BbsI Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstXI BstYI BtgI DrdI EaeI EarI Eco57MI EcoRI FalI HaeII Hin4I HincII MfeI MmeI MslI MspA1I NdeI NheI NspI PciI PflMI PfoI PmlI PsiI PstI PvuII SexAI SfcI SmaI SmlI SrfI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI BsaI BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PasI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TatI Tth111I XbaI XhoI ZraI