################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 931 CGCG CG^CG AspHI 940 GTGCTC G[AT]GC[AT]^C AspI 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 495 GGATCC G^GATCC BbvCI 289 GCTGAGG CCTCAGC(-5/-2) BbvI 709 GCAGC GCAGC(8/12) BcnI 546 CCGGG CC^[GC]GG BpmI 141 CTGGAG CTGGAG(16/14) BsaI 1049 GGTCTC GGTCTC(1/5) BseRI 777 CTCCTC GAGGAG(10/8) BsiHKAI 940 GTGCTC G[AT]GC[AT]^C BsmFI 416 GTCCC GGGAC(10/14) BstNI 140 CCTGG CC^[AT]GG BstUI 931 CGCG CG^CG BstYI 495 GGATCC [GA]^GATC[CT] Cfr13I 1373 GGGCC G^G[ACGT]CC DraI 670 TTTAAA TTT^AAA EcoRII 140 CCTGG ^CC[AT]GG Fnu4HI 709 GCAGC GC^[ACGT]GC HaeIII 1374 GGCC GG^CC HgaI 238 GACGC GACGC(5/10) HincII 1366 GTCAAC GT[CT]^[GA]AC HindII 1366 GTCAAC GT[CT]^[GA]AC ItaI 709 GCAGC GC^[ACGT]GC MaeII 593 ACGT A^CGT MflI 495 GGATCC [GA]^GATC[CT] MvaI 140 CCTGG CC^[AT]GG MvnI 931 CGCG CG^CG NciI 546 CCGGG CC^[GC]GG NruI 930 TCGCGA TCG^CGA PflFI 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Sau96I 1373 GGGCC G^G[ACGT]CC SspI 1001 AATATT AAT^ATT TfiI 1557 GATTC G^A[AT]TC TseI 709 GCAGC G^C[AT]GC Tth111I 1360 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 495 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Asp700I 627 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1324 GAAAAGCTTC BfaI 1054 CTAG C^TAG 1657 CTAG BsiYI 546 CCGGGATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1097 CCATGAAGCGG BslI 546 CCGGGATCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1097 CCATGAAGCGG BssKI 140 CCTGG ^CC[ACGT]GG 546 CCGGG EarI 1377 CTCTTC CTCTTC(1/4) 442 GAAGAG EcoT14I 586 CCTTGG C^C[AT][AT]GG 1140 CCTTGG EcoT22I 333 ATGCAT ATGCA^T 1307 ATGCAT HapII 12 CCGG C^CGG 546 CCGG HpaII 12 CCGG C^CGG 546 CCGG HphI 361 GGTGA GGTGA(8/7) 1247 TCACC Ksp632I 1377 CTCTTC CTCTTC(1/4) 442 GAAGAG MaeI 1054 CTAG C^TAG 1657 CTAG MslI 336 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1150 CATCTGGATG MspI 12 CCGG C^CGG 546 CCGG NsiI 333 ATGCAT ATGCA^T 1307 ATGCAT NspI 885 GCATGT [GA]CATG^[CT] 1534 ACATGC PstI 126 CTGCAG CTGCA^G 423 CTGCAG ScrFI 140 CCTGG CC^[ACGT]GG 546 CCGGG StyI 586 CCTTGG C^C[AT][AT]GG 1140 CCTTGG XmnI 627 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1324 GAAAAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanII BbsI BmyI BpuAI BsaJI BsmAI Bsp1286I BsrI BstF5I Cac8I DdeI DpnII FokI HindIII HinfI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI SfaNI SfcI Tru9I Tsp45I Tsp509I TspRI