Restriction map for pFN21AE1314
 (Circular) (Six-base) MAPSORT of: pFN21AE1314.seq  Check: 755  from: 1  to: 6382



pFN21AE1314  6382 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:43  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4192     278 
   Size:         53      83     186     936    2656    2468

  Fragments arranged by size: 

                2656    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3725    1937 
   Size:       1788    4594
 
Acc65I G'GTAC_C

Cuts at:   3703    3703 
   Size:       6382
 
AclI AA'CG_TT

Cuts at:   1563    4509    4882    1563 
   Size:       2946     373    3063

  Fragments arranged by size: 

                3063    2946     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3666    4445    5829    6097    1632 
   Size:       2034     779    1384     268    1917

  Fragments arranged by size: 

                2034    1917    1384     779     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6185
 
AflIII A'CryG_T

Cuts at:   1203    5281    6258    1203 
   Size:       4078     977    1327

  Fragments arranged by size: 

                4078    1327     977
 
AgeI A'CCGG_T

Cuts at:   5213    5213 
   Size:       6382
 
AhdI GACnn_n'nnGTC

Cuts at:   5111    5111 
   Size:       6382
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6382
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2363    1184 
   Size:       1179    5203
 
AlwNI CAG_nnn'CTG

Cuts at:   4125    5697    4125 
   Size:       1572    4810
 
ApaLI G'TGCA_C

Cuts at:   4439    5595    4439 
   Size:       1156    5226
 
ApoI r'AATT_y

Cuts at:   1493    2635    2704    2729    2904    3200    3515    3602    3639 
   Size:       1142      69      25     175     296     315      87      37

Cuts at:   3639    3691    3917    4069    6178    1493 
   Size:         52     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1142     315     296     226     175     152
                  87      69      52      37      25
 
AseI AT'TA_AT

Cuts at:    160    1032    4936     160 
   Size:        872    3904    1606

  Fragments arranged by size: 

                3904    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6382
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3707    1870 
   Size:         73    1764    4545

  Fragments arranged by size: 

                4545    1764      73
 
BamHI G'GATC_C

Cuts at:   2500    3712    4079    2500 
   Size:       1212     367    4803

  Fragments arranged by size: 

                4803    1212     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3703    5158     618 
   Size:        325     217     627    1916    1455    1842

  Fragments arranged by size: 

                1916    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2144    2283    2418    3701    1391 
   Size:        753     139     135    1283    4072

  Fragments arranged by size: 

                4072    1283     753     139     135
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2593    2702    3507     928 
   Size:        971     694     109     805    3803

  Fragments arranged by size: 

                3803     971     805     694     109
 
BbvCI CC'TCA_GC

Cuts at:   2295    2295 
   Size:       6382
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4573    4607    1903 
   Size:         34    2636      34    3678

  Fragments arranged by size: 

                3678    2636      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4274    5484    4274 
   Size:       1210    5172
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6382
 
BfrBI ATG'CAT

Cuts at:   2340    3314    2340 
   Size:        974    5408
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4993     136 
   Size:        107     122      71     631     605    3321    1525

  Fragments arranged by size: 

                3321    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6377    6377 
   Size:       6382
 
BlpI GC'TnA_GC

Cuts at:   3799    3799 
   Size:       6382
 
Bme1580I G_kGCm'C

Cuts at:   1790    2274    4443    5599    1790 
   Size:        484    2169    1156    2573

  Fragments arranged by size: 

                2573    2169    1156     484
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6382
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3385    4113    5071 
   Size:        416      53      83     399      24    1968     728     958

Cuts at:   5071     442 
   Size:       1753

  Fragments arranged by size: 

                1968    1753     958     728     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6382
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2167    5042    1239 
   Size:        928    2875    2579

  Fragments arranged by size: 

                2875    2579     928
 
Bpu10I CC'TnA_GC

Cuts at:   2295    2507    2295 
   Size:        212    6170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4507    5372    5670    4507 
   Size:        865     298    5219

  Fragments arranged by size: 

                5219     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3060    5045     882 
   Size:        617    1561    1985    2219

  Fragments arranged by size: 

                2219    1985    1561     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4173     493 
   Size:        711    2969    2702

  Fragments arranged by size: 

                2969    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6376    6376 
   Size:       6382
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4189    4571    5206     275 
   Size:         53      83     186     936    2656     382     635    1451

  Fragments arranged by size: 

                2656    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4815    5213    5487    5634    6045    4815 
   Size:        398     274     147     411    5152

  Fragments arranged by size: 

                5152     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3543    3573    1178 
   Size:         30     165      30    2140      30    3987

  Fragments arranged by size: 

                3987    2140     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2773    1186 
   Size:       1587    4795
 
BseYI C'CCAG_C

Cuts at:   5585    6081    5585 
   Size:        496    5886
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5809
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3832    4593    5621    1540 
   Size:        275     186    1831     761    1028    2301

  Fragments arranged by size: 

                2301    1831    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2949    4443    4528    5599    2949 
   Size:       1494      85    1071    3732

  Fragments arranged by size: 

                3732    1494    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3893    3986    3893 
   Size:         93    6289
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2144    2274    2283    2418    2949    3701    4443 
   Size:        399     354     130       9     135     531     752     742

Cuts at:   4443    4528    5599    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     752     742     531     399     354     135
                 130      85       9
 
BspEI T'CCGG_A

Cuts at:   6045    6045 
   Size:       6382
 
BspHI T'CATG_A

Cuts at:   4271    6001    4271 
   Size:       1730    4652
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3737     844 
   Size:       2893    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4269    1394 
   Size:        429    2446    3507

  Fragments arranged by size: 

                3507    2446     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4877    5051      65 
   Size:       1183    3629     174    1396

  Fragments arranged by size: 

                3629    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5026    5213    1700 
   Size:        110    3216     187    2869

  Fragments arranged by size: 

                3216    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6382
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6382
 
BssSI C'ACGA_G

Cuts at:   4442    5454    4442 
   Size:       1012    5370
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6382
 
BstBI TT'CG_AA

Cuts at:   5186    5186 
   Size:       6382
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6382
 
BstYI r'GATC_y

Cuts at:   1972    2500    3712    4079    4464    4481    5933    6019    6031 
   Size:        528    1212     367     385      17    1452      86      12

Cuts at:   6031    6377    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1212     528     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5830
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2952     487 
   Size:        683      56    1726    3917

  Fragments arranged by size: 

                3917    1726     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2113    3898    4692    4712    2113 
   Size:       1785     794      20    3783

  Fragments arranged by size: 

                3783    1785     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6382
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2943    2978     529 
   Size:         35    2379      35    3933

  Fragments arranged by size: 

                3933    2379      35      35
 
DraI TTT'AAA

Cuts at:   2677    3686    2677 
   Size:       1009    5373
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5389     809 
   Size:        507    4073    1802

  Fragments arranged by size: 

                4073    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3829    4718    6347 
   Size:         54    1425      50     275      18    1999     889    1629

Cuts at:   6347       8 
   Size:         43

  Fragments arranged by size: 

                1999    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3829    1537 
   Size:        275    2017    4090

  Fragments arranged by size: 

                4090    2017     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2442    3388    4312    1353 
   Size:       1089     946     924    3423

  Fragments arranged by size: 

                3423    1089     946     924
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4969    5343    5489     387 
   Size:       1064     138    3380     374     146    1280

  Fragments arranged by size: 

                3380    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2167    3666    4445    5042    5829    6097    1239 
   Size:        393     535    1499     779     597     787     268    1524

  Fragments arranged by size: 

                1524    1499     787     779     597     535     393     268
            
 
EcoRI G'AATT_C

Cuts at:   3691    4069    3691 
   Size:        378    6004
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2532    3350    2532 
   Size:        818    5564
 
FspI TGC'GCA

Cuts at:   4888    4888 
   Size:       6382
 
HaeII r_GCGC'y

Cuts at:   1678    5529    1678 
   Size:       3851    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2684    2716    5023    5055    5097 
   Size:         32     135      32    1112      32    2307      32      42

Cuts at:   5097    5129    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2307    1112     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3373    3726    3972     669 
   Size:        988     281    1435     353     246    3079

  Fragments arranged by size: 

                3079    1435     988     353     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2976    3096    3332     748 
   Size:       2228     120     236    3798

  Fragments arranged by size: 

                3798    2228     236     120
 
HpaI GTT'AAC

Cuts at:   3972    3972 
   Size:       6382
 
KpnI G_GTAC'C

Cuts at:   3707    3707 
   Size:       6382
 
MfeI C'AATT_G

Cuts at:   3981    3981 
   Size:       6382
 
MluI A'CGCG_T

Cuts at:   6258    6258 
   Size:       6382
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3172    5496    5680    1305 
   Size:       1867    2324     184    2007

  Fragments arranged by size: 

                2324    2007    1867     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4560

  Fragments arranged by size: 

                4560    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2345    3159    4340    4699    4858     518 
   Size:        648    1179     814    1181     359     159    2042

  Fragments arranged by size: 

                2042    1181    1179     814     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3798    4475    5623    5868    1606 
   Size:       2192     677    1148     245    2120

  Fragments arranged by size: 

                2192    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5830
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6382
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6382
 
NotI GC'GGCC_GC

Cuts at:   3829    3829 
   Size:       6382
 
NruI TCG'CGA

Cuts at:   2937    2937 
   Size:       6382
 
NsiI A_TGCA'T

Cuts at:   2342    3316    2342 
   Size:        974    5408
 
NspI r_CATG'y

Cuts at:   1132    1229    2894    3543    3740    5285    1132 
   Size:         97    1665     649     197    1545    2229

  Fragments arranged by size: 

                2229    1665    1545     649     197      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6382
 
PciI A'CATG_T

Cuts at:   5281    5281 
   Size:       6382
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6382
 
PmeI GTTT'AAAC

Cuts at:   3686    3686 
   Size:       6382
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4451    4483    5964    5996    4451 
   Size:         32    1481      32    4837

  Fragments arranged by size: 

                4837    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6382
 
PsiI TTA'TAA

Cuts at:   3952    3952 
   Size:       6382
 
PstI C_TGCA'G

Cuts at:    830    2135    2432    3734     830 
   Size:       1305     297    1302    3478

  Fragments arranged by size: 

                3478    1305    1302     297
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6382
 
SalI G'TCGA_C

Cuts at:   1936    3724    1936 
   Size:       1788    4594
 
SbfI CC_TGCA'GG

Cuts at:   3734    3734 
   Size:       6382
 
ScaI AGT'ACT

Cuts at:   4630    6342    4630 
   Size:       1712    4670
 
SfcI C'TryA_G

Cuts at:    826    1045    2131    2428    3040    3730    4865    5546    5737 
   Size:        219    1086     297     612     690    1135     681     191

Cuts at:   5737     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1086     690     681     612     297     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6382
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6382
 
SmaI CCC'GGG

Cuts at:   1872    3709    1872 
   Size:       1837    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4486    5387    5649     820 
   Size:        197     926    2543     901     262    1553

  Fragments arranged by size: 

                2543    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4173     493 
   Size:       3680    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6382
 
SphI G_CATG'C

Cuts at:   1132    3740    1132 
   Size:       2608    3774
 
SspI AAT'ATT

Cuts at:      5      52    3008    4306       5 
   Size:         47    2956    1298    2081

  Fragments arranged by size: 

                2956    2081    1298      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6382
 
StyI C'CwwG_G

Cuts at:    513    1065    2591    3145    3821     513 
   Size:        552    1526     554     676    3074

  Fragments arranged by size: 

                3074    1526     676     554     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2752    4409    4426    4579    4764    5209     325 
   Size:       1255    1172    1657      17     153     185     445    1498

  Fragments arranged by size: 

                1657    1498    1255    1172     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2070    2327    4628 
   Size:        274      80      33      51    1443      93     257    2301

Cuts at:   4628    6340      96 
   Size:       1712     138

  Fragments arranged by size: 

                2301    1712    1443     274     257     138      93      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3543    3563    3575    3595    6342    6374    3543 
   Size:         20      12      20    2747      32    3551

  Fragments arranged by size: 

                3551    2747      32      20      20      12
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3368    1316 
   Size:       2052    4330
 
XbaI T'CTAG_A

Cuts at:   3718    3718 
   Size:       6382
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6382
 
XmaI C'CCGG_G

Cuts at:   1870    3707    1870 
   Size:       1837    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2636    3333    4511    2636 
   Size:        697    1178    4507

  Fragments arranged by size: 

                4507    1178     697
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4190     276 
   Size:         53      83     186     936    2656    2468

  Fragments arranged by size: 

                2656    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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