(Circular) (Six-base) MAPSORT of: pFN21AE1314.seq Check: 755 from: 1 to: 6382 pFN21AE1314 6382 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3666 4445 5829 6097 1632 Size: 2034 779 1384 268 1917 Fragments arranged by size: 2034 1917 1384 779 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 5281 6258 1203 Size: 4078 977 1327 Fragments arranged by size: 4078 1327 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2363 1184 Size: 1179 5203 AlwNI CAG_nnn'CTG Cuts at: 4125 5697 4125 Size: 1572 4810 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 2635 2704 2729 2904 3200 3515 3602 3639 Size: 1142 69 25 175 296 315 87 37 Cuts at: 3639 3691 3917 4069 6178 1493 Size: 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 1142 315 296 226 175 152 87 69 52 37 25 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 3707 1870 Size: 73 1764 4545 Fragments arranged by size: 4545 1764 73 BamHI G'GATC_C Cuts at: 2500 3712 4079 2500 Size: 1212 367 4803 Fragments arranged by size: 4803 1212 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3703 5158 618 Size: 325 217 627 1916 1455 1842 Fragments arranged by size: 1916 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2144 2283 2418 3701 1391 Size: 753 139 135 1283 4072 Fragments arranged by size: 4072 1283 753 139 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2593 2702 3507 928 Size: 971 694 109 805 3803 Fragments arranged by size: 3803 971 805 694 109 BbvCI CC'TCA_GC Cuts at: 2295 2295 Size: 6382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 1903 Size: 34 2636 34 3678 Fragments arranged by size: 3678 2636 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 1548 Size: 6382 BfrBI ATG'CAT Cuts at: 2340 3314 2340 Size: 974 5408 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4993 136 Size: 107 122 71 631 605 3321 1525 Fragments arranged by size: 3321 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 2274 4443 5599 1790 Size: 484 2169 1156 2573 Fragments arranged by size: 2573 2169 1156 484 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3385 4113 5071 Size: 416 53 83 399 24 1968 728 958 Cuts at: 5071 442 Size: 1753 Fragments arranged by size: 1968 1753 958 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2167 5042 1239 Size: 928 2875 2579 Fragments arranged by size: 2875 2579 928 Bpu10I CC'TnA_GC Cuts at: 2295 2507 2295 Size: 212 6170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4507 5372 5670 4507 Size: 865 298 5219 Fragments arranged by size: 5219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3060 5045 882 Size: 617 1561 1985 2219 Fragments arranged by size: 2219 1985 1561 617 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4189 4571 5206 275 Size: 53 83 186 936 2656 382 635 1451 Fragments arranged by size: 2656 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4815 5213 5487 5634 6045 4815 Size: 398 274 147 411 5152 Fragments arranged by size: 5152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3543 3573 1178 Size: 30 165 30 2140 30 3987 Fragments arranged by size: 3987 2140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2773 1186 Size: 1587 4795 BseYI C'CCAG_C Cuts at: 5585 6081 5585 Size: 496 5886 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5809 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2949 4443 4528 5599 2949 Size: 1494 85 1071 3732 Fragments arranged by size: 3732 1494 1071 85 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2274 2283 2418 2949 3701 4443 Size: 399 354 130 9 135 531 752 742 Cuts at: 4443 4528 5599 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 531 399 354 135 130 85 9 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 4271 6001 4271 Size: 1730 4652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3737 844 Size: 2893 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4269 1394 Size: 429 2446 3507 Fragments arranged by size: 3507 2446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4877 5051 65 Size: 1183 3629 174 1396 Fragments arranged by size: 3629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5026 5213 1700 Size: 110 3216 187 2869 Fragments arranged by size: 3216 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstYI r'GATC_y Cuts at: 1972 2500 3712 4079 4464 4481 5933 6019 6031 Size: 528 1212 367 385 17 1452 86 12 Cuts at: 6031 6377 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1212 528 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5830 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2952 487 Size: 683 56 1726 3917 Fragments arranged by size: 3917 1726 683 56 BtsI GCAGTG_nn' Cuts at: 2113 3898 4692 4712 2113 Size: 1785 794 20 3783 Fragments arranged by size: 3783 1785 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2943 2978 529 Size: 35 2379 35 3933 Fragments arranged by size: 3933 2379 35 35 DraI TTT'AAA Cuts at: 2677 3686 2677 Size: 1009 5373 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5389 809 Size: 507 4073 1802 Fragments arranged by size: 4073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3829 4718 6347 Size: 54 1425 50 275 18 1999 889 1629 Cuts at: 6347 8 Size: 43 Fragments arranged by size: 1999 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2442 3388 4312 1353 Size: 1089 946 924 3423 Fragments arranged by size: 3423 1089 946 924 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4969 5343 5489 387 Size: 1064 138 3380 374 146 1280 Fragments arranged by size: 3380 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 3666 4445 5042 5829 6097 1239 Size: 393 535 1499 779 597 787 268 1524 Fragments arranged by size: 1524 1499 787 779 597 535 393 268 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2532 3350 2532 Size: 818 5564 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 5529 1678 Size: 3851 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2684 2716 5023 5055 5097 Size: 32 135 32 1112 32 2307 32 42 Cuts at: 5097 5129 1373 Size: 32 2626 Fragments arranged by size: 2626 2307 1112 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3373 3726 3972 669 Size: 988 281 1435 353 246 3079 Fragments arranged by size: 3079 1435 988 353 281 246 HindIII A'AGCT_T Cuts at: 748 2976 3096 3332 748 Size: 2228 120 236 3798 Fragments arranged by size: 3798 2228 236 120 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3172 5496 5680 1305 Size: 1867 2324 184 2007 Fragments arranged by size: 2324 2007 1867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4560 Fragments arranged by size: 4560 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2345 3159 4340 4699 4858 518 Size: 648 1179 814 1181 359 159 2042 Fragments arranged by size: 2042 1181 1179 814 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3798 4475 5623 5868 1606 Size: 2192 677 1148 245 2120 Fragments arranged by size: 2192 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5830 NdeI CA'TA_TG Cuts at: 387 387 Size: 6382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NruI TCG'CGA Cuts at: 2937 2937 Size: 6382 NsiI A_TGCA'T Cuts at: 2342 3316 2342 Size: 974 5408 NspI r_CATG'y Cuts at: 1132 1229 2894 3543 3740 5285 1132 Size: 97 1665 649 197 1545 2229 Fragments arranged by size: 2229 1665 1545 649 197 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 5281 5281 Size: 6382 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PstI C_TGCA'G Cuts at: 830 2135 2432 3734 830 Size: 1305 297 1302 3478 Fragments arranged by size: 3478 1305 1302 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SfcI C'TryA_G Cuts at: 826 1045 2131 2428 3040 3730 4865 5546 5737 Size: 219 1086 297 612 690 1135 681 191 Cuts at: 5737 826 Size: 1471 Fragments arranged by size: 1471 1135 1086 690 681 612 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 3709 1872 Size: 1837 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4486 5387 5649 820 Size: 197 926 2543 901 262 1553 Fragments arranged by size: 2543 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 3008 4306 5 Size: 47 2956 1298 2081 Fragments arranged by size: 2956 2081 1298 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6382 StyI C'CwwG_G Cuts at: 513 1065 2591 3145 3821 513 Size: 552 1526 554 676 3074 Fragments arranged by size: 3074 1526 676 554 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2752 4409 4426 4579 4764 5209 325 Size: 1255 1172 1657 17 153 185 445 1498 Fragments arranged by size: 1657 1498 1255 1172 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 2327 4628 Size: 274 80 33 51 1443 93 257 2301 Cuts at: 4628 6340 96 Size: 1712 138 Fragments arranged by size: 2301 1712 1443 274 257 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3543 3563 3575 3595 6342 6374 3543 Size: 20 12 20 2747 32 3551 Fragments arranged by size: 3551 2747 32 20 20 12 Tth111I GACn'n_nGTC Cuts at: 1316 3368 1316 Size: 2052 4330 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 3707 1870 Size: 1837 4545 XmnI GAAnn'nnTTC Cuts at: 2636 3333 4511 2636 Size: 697 1178 4507 Fragments arranged by size: 4507 1178 697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI