(Linear) (Six-base) MAPSORT of: KIEE1314.seq Check: 1049 from: 1 to: 1677 KIEE1314.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1661 1677 Size: 1661 16 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 358 1677 Size: 358 1319 ApoI r'AATT_y Cuts at: 0 630 699 724 899 1195 1510 1597 1634 Size: 630 69 25 175 296 315 87 37 Cuts at: 1634 1677 Size: 43 Fragments arranged by size: 630 315 296 175 87 69 43 37 25 BamHI G'GATC_C Cuts at: 0 495 1677 Size: 495 1182 BanII G_rGCy'C Cuts at: 0 139 278 413 1677 Size: 139 139 135 1264 Fragments arranged by size: 1264 139 139 135 BbsI GAAGACnn'nnnn_ Cuts at: 0 588 697 1502 1677 Size: 588 109 805 175 Fragments arranged by size: 805 588 175 109 BbvCI CC'TCA_GC Cuts at: 0 290 1677 Size: 290 1387 BfrBI ATG'CAT Cuts at: 0 335 1309 1677 Size: 335 974 368 Fragments arranged by size: 974 368 335 Bme1580I G_kGCm'C Cuts at: 0 269 1677 Size: 269 1408 BmrI ACTGGGnnnn_n' Cuts at: 0 1380 1677 Size: 1380 297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 1677 Size: 162 1515 Bpu10I CC'TnA_GC Cuts at: 0 290 502 1677 Size: 290 212 1175 Fragments arranged by size: 1175 290 212 BsaI GGTCTCn'nnnn_ Cuts at: 0 1055 1677 Size: 1055 622 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1538 1568 1677 Size: 1538 30 109 Fragments arranged by size: 1538 109 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 768 1677 Size: 768 909 BsiHKAI G_wGCw'C Cuts at: 0 944 1677 Size: 944 733 Bsp1286I G_dGCh'C Cuts at: 0 139 269 278 413 944 1677 Size: 139 130 9 135 531 733 Fragments arranged by size: 733 531 139 135 130 9 BstYI r'GATC_y Cuts at: 0 495 1677 Size: 495 1182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 947 1677 Size: 947 730 BtsI GCAGTG_nn' Cuts at: 0 108 1677 Size: 108 1569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 938 973 1677 Size: 938 35 704 Fragments arranged by size: 938 704 35 DraI TTT'AAA Cuts at: 0 672 1677 Size: 672 1005 EarI CTCTTCn'nnn_ Cuts at: 0 437 1383 1677 Size: 437 946 294 Fragments arranged by size: 946 437 294 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 1661 1677 Size: 162 1499 16 Fragments arranged by size: 1499 162 16 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 527 1345 1677 Size: 527 818 332 Fragments arranged by size: 818 527 332 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 679 711 1677 Size: 679 32 966 Fragments arranged by size: 966 679 32 HincII GTy'rAC Cuts at: 0 1368 1677 Size: 1368 309 HindIII A'AGCT_T Cuts at: 0 971 1091 1327 1677 Size: 971 120 236 350 Fragments arranged by size: 971 350 236 120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1167 1677 Size: 1167 510 MslI CAynn'nnrTG Cuts at: 0 340 1154 1677 Size: 340 814 523 Fragments arranged by size: 814 523 340 NruI TCG'CGA Cuts at: 0 932 1677 Size: 932 745 NsiI A_TGCA'T Cuts at: 0 337 1311 1677 Size: 337 974 366 Fragments arranged by size: 974 366 337 NspI r_CATG'y Cuts at: 0 889 1538 1677 Size: 889 649 139 Fragments arranged by size: 889 649 139 PstI C_TGCA'G Cuts at: 0 130 427 1677 Size: 130 297 1250 Fragments arranged by size: 1250 297 130 SfcI C'TryA_G Cuts at: 0 126 423 1035 1677 Size: 126 297 612 642 Fragments arranged by size: 642 612 297 126 SspI AAT'ATT Cuts at: 0 1003 1677 Size: 1003 674 StyI C'CwwG_G Cuts at: 0 586 1140 1677 Size: 586 554 537 Fragments arranged by size: 586 554 537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 747 1677 Size: 747 930 TatI w'GTAC_w Cuts at: 0 65 322 1677 Size: 65 257 1355 Fragments arranged by size: 1355 257 65 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1538 1558 1570 1590 1677 Size: 1538 20 12 20 87 Fragments arranged by size: 1538 87 20 20 12 Tth111I GACn'n_nGTC Cuts at: 0 1363 1677 Size: 1363 314 XmnI GAAnn'nnTTC Cuts at: 0 631 1328 1677 Size: 631 697 349 Fragments arranged by size: 697 631 349 Enzymes that do cut: AcuI AlfI ApoI BamHI BanII BbsI BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI BsiHKAI Bsp1286I BstYI BtgZI BtsI CspCI DraI EarI Eco57MI FalI Hin4I HincII HindIII MmeI MslI NruI NsiI NspI PstI SfcI SspI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI XmaI ZraI