Restriction map for
pFN21AE6424
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaI BsiWI
BsmBI BsmI BspDI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1408 GTCTAC GT^[AC][GT]AC
AccIII 642 TCCGGA T^CCGGA
Alw44I 1378 GTGCAC G^TGCAC
AocI 659 CCTCAGG CC^T[ACGT]AGG
ApaLI 1378 GTGCAC G^TGCAC
Asp700I 1356 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 59 GGCGCC GGCGC^C
BbrPI 114 CACGTG CAC^GTG
BbsI 1058 GAAGAC GAAGAC(2/6)
BbvCI 1549 GCTGAGG CCTCAGC(-5/-2)
BcgI 83 CGAGCACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 618 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 618 GCTCAGC GC^T[ACGT]AGC
BpuAI 1058 GAAGAC GAAGAC(2/6)
BsaAI 114 CACGTG [CT]AC^GT[GA]
BsaWI 642 TCCGGA [AT]^CCGG[AT]
BseAI 642 TCCGGA T^CCGGA
BsgI 1526 CTGCAC GTGCAG(16/14)
Bsp1407I 416 TGTACA T^GTACA
BspEI 642 TCCGGA T^CCGGA
BspHI 512 TCATGA T^CATGA
BspLU11I 1478 ACATGT A^CATGT
BsrDI 1508 CATTGC GCAATG(2/0)
BsrGI 416 TGTACA T^GTACA
BstEII 1113 GGTGACC G^GT[ACGT]ACC
BstPI 1113 GGTGACC G^GT[ACGT]ACC
Bsu36I 659 CCTCAGG CC^T[ACGT]AGG
CelII 618 GCTCAGC GC^T[ACGT]AGC
DraIII 64 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 659 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1113 GGTGACC G^GT[ACGT]ACC
KasI 59 GGCGCC G^GCGCC
MroI 642 TCCGGA T^CCGGA
NarI 59 GGCGCC GG^CGCC
PaeR7I 303 CTCGAG C^TCGAG
PleI 316 GACTC GAGTC(4/5)
PmaCI 114 CACGTG CAC^GTG
PmlI 114 CACGTG CAC^GTG
RcaI 512 TCATGA T^CATGA
SacI 616 GAGCTC GAGCT^C
SfaNI 278 GCATC GCATC(5/9)
SfiI 770 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 59 GGCGCC GGC^GCC
SphI 1434 GCATGC GCATG^C
SspBI 416 TGTACA T^GTACA
TfiI 607 GAATC G^A[AT]TC
XhoI 303 CTCGAG C^TCGAG
XmnI 1356 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 59 GGCGCC G[GA]^CG[CT]C
78 GACGCC
AfaI 417 GTAC GT^AC
1476 GTAC
AflIII 113 ACACGT A^C[GA][CT]GT
1478 ACATGT
Aor51HI 589 AGCGCT AGC^GCT
1157 AGCGCT
BamHI 867 GGATCC G^GATCC
903 GGATCC
BsaHI 59 GGCGCC G[GA]^CG[CT]C
78 GACGCC
BspMI 206 ACCTGC ACCTGC(4/8)
1402 GCAGGT
BsrI 189 CCAGT ACTGG(1/-1)
406 CCAGT
BssSI 420 CACGAG CACGAG(-5/-1)
774 CACGAG
BstF5I 1581 CATCC GGATG(2/0)
426 GGATG
BstYI 867 GGATCC [GA]^GATC[CT]
903 GGATCC
DrdI 462 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1195 GACCGCTACGTC
EarI 1360 CTCTTC CTCTTC(1/4)
1605 GAAGAG
Eco47III 589 AGCGCT AGC^GCT
1157 AGCGCT
EcoNI 1344 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1614 CCTGGGCAAGG
FokI 1581 CATCC GGATG(9/13)
426 GGATG
Hin1I 59 GGCGCC G[GA]^CG[CT]C
78 GACGCC
HinfI 316 GACTC G^A[ACGT]TC
607 GAATC
Ksp632I 1360 CTCTTC CTCTTC(1/4)
1605 GAAGAG
KspI 29 CCGCGG CCGC^GG
624 CCGCGG
MflI 867 GGATCC [GA]^GATC[CT]
903 GGATCC
NspI 1434 GCATGC [GA]CATG^[CT]
1478 ACATGT
PpuMI 1181 GGGTCCT [GA]G^G[AT]CC[CT]
1495 GGGTCCC
RsaI 417 GTAC GT^AC
1476 GTAC
SacII 29 CCGCGG CCGC^GG
624 CCGCGG
TaqI 176 TCGA T^CGA
304 TCGA
TspRI 190 CAGTG CA[GC]TG[ACGT][ACGT]^
1235 CAGTG
TthHB8I 176 TCGA T^CGA
304 TCGA
XcmI 221 CCAAGCTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1226 CCAGCGAGACAGTGG
XhoII 867 GGATCC [GA]^GATC[CT]
903 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfcI SmlI StuI StyI
TseI Tsp45I