(Circular) (Six-base) MAPSORT of: pFN21AE6424.seq Check: 672 from: 1 to: 6364 pFN21AE6424 6364 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3398 3398 Size: 6364 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3414 3707 1937 Size: 1477 293 4594 Fragments arranged by size: 4594 1477 293 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2180 2784 3115 3662 4427 5811 6079 Size: 455 93 604 331 547 765 1384 268 Cuts at: 6079 1632 Size: 1917 Fragments arranged by size: 1917 1384 765 604 547 455 331 268 93 AfeI AGC'GCT Cuts at: 2596 3164 2596 Size: 568 5796 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2118 3483 5263 6240 1203 Size: 915 1365 1780 977 1327 Fragments arranged by size: 1780 1365 1327 977 915 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2114 2306 2486 1184 Size: 930 192 180 5062 Fragments arranged by size: 5062 930 192 180 AlwNI CAG_nnn'CTG Cuts at: 2110 2479 3524 3554 4107 5679 2110 Size: 369 1045 30 553 1572 2795 Fragments arranged by size: 2795 1572 1045 553 369 30 ApaI G_GGCC'C Cuts at: 2673 2768 2778 2673 Size: 95 10 6259 Fragments arranged by size: 6259 95 10 ApaLI G'TGCA_C Cuts at: 3383 4421 5577 3383 Size: 1038 1156 4170 Fragments arranged by size: 4170 1156 1038 ApoI r'AATT_y Cuts at: 1493 3673 3899 4051 6160 1493 Size: 2180 226 152 2109 1697 Fragments arranged by size: 2180 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2308 2740 3284 3689 1870 Size: 73 365 432 544 405 4545 Fragments arranged by size: 4545 544 432 405 365 73 BamHI G'GATC_C Cuts at: 2872 2908 3694 4061 2872 Size: 36 786 367 5175 Fragments arranged by size: 5175 786 367 36 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 2239 2710 3628 3685 Size: 325 217 627 277 175 471 918 57 Cuts at: 3685 5140 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 918 627 471 325 277 217 175 57 BanII G_rGCy'C Cuts at: 1391 2564 2625 2673 2768 2778 2955 3585 3683 Size: 1173 61 48 95 10 177 630 98 Cuts at: 3683 1391 Size: 4072 Fragments arranged by size: 4072 1173 630 177 98 95 61 48 10 BbeI G_GCGC'C Cuts at: 2068 2068 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3070 928 Size: 971 1171 4222 Fragments arranged by size: 4222 1171 971 BbvCI CC'TCA_GC Cuts at: 3555 3555 Size: 6364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2077 2111 4555 4589 1903 Size: 34 140 34 2444 34 3678 Fragments arranged by size: 3678 2444 140 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2782 2835 Size: 107 122 71 631 605 337 773 53 Cuts at: 2835 3637 4975 136 Size: 802 1338 1525 Fragments arranged by size: 1525 1338 802 773 631 605 337 122 107 71 53 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2624 3781 2624 Size: 1157 5207 Bme1580I G_kGCm'C Cuts at: 1790 2244 2465 2673 2713 2768 2778 3387 3633 Size: 454 221 208 40 55 10 609 246 Cuts at: 3633 4425 5581 1790 Size: 792 1156 2573 Fragments arranged by size: 2573 1156 792 609 454 246 221 208 55 40 10 BmgBI CAC'GTC Cuts at: 1318 3058 3466 1318 Size: 1740 408 4216 Fragments arranged by size: 4216 1740 408 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2405 4095 5053 Size: 416 53 83 399 24 988 1690 958 Cuts at: 5053 442 Size: 1753 Fragments arranged by size: 1753 1690 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2177 2252 2809 2853 3011 3179 3440 Size: 893 45 75 557 44 158 168 261 Cuts at: 3440 3455 5024 1239 Size: 15 1569 2579 Fragments arranged by size: 2579 1569 893 557 261 168 158 75 45 44 15 Bpu10I CC'TnA_GC Cuts at: 3555 3579 3555 Size: 24 6340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2659 3065 4489 5354 5652 2659 Size: 406 1424 865 298 3371 Fragments arranged by size: 3371 1424 865 406 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5027 882 Size: 617 3528 2219 Fragments arranged by size: 3528 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2121 4155 493 Size: 711 917 2034 2702 Fragments arranged by size: 2702 2034 917 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 2084 4171 4553 Size: 53 83 186 936 532 19 2087 382 Cuts at: 4553 5188 275 Size: 635 1451 Fragments arranged by size: 2087 1451 936 635 532 382 186 83 53 19 BsaWI w'CCGG_w Cuts at: 2647 4797 5195 5469 5616 6027 2647 Size: 2150 398 274 147 411 2984 Fragments arranged by size: 2984 2150 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2105 2135 1178 Size: 30 165 30 702 30 5407 Fragments arranged by size: 5407 702 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2185 3008 3280 3413 3572 3628 1186 Size: 999 823 272 133 159 56 3922 Fragments arranged by size: 3922 999 823 272 159 133 56 BseYI C'CCAG_C Cuts at: 2479 2877 2913 3075 3230 3600 5567 6063 2479 Size: 398 36 162 155 370 1967 496 2780 Fragments arranged by size: 2780 1967 496 398 370 162 155 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3516 1163 Size: 573 1780 4011 Fragments arranged by size: 4011 1780 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2062 3202 3375 3462 3814 4575 Size: 275 186 61 1140 173 87 352 761 Cuts at: 4575 5603 1540 Size: 1028 2301 Fragments arranged by size: 2301 1140 1028 761 352 275 186 173 87 61 BsiHKAI G_wGCw'C Cuts at: 2093 2181 2625 2685 2705 3387 4425 4510 5581 Size: 88 444 60 20 682 1038 85 1071 Cuts at: 5581 2093 Size: 2876 Fragments arranged by size: 2876 1071 1038 682 444 88 85 60 20 BsmI GAATG_Cn' Cuts at: 3875 3968 3875 Size: 93 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2181 2244 2465 2564 2625 2673 Size: 399 303 88 63 221 99 61 48 Cuts at: 2673 2685 2705 2713 2768 2778 2955 3387 3585 Size: 12 20 8 55 10 177 432 198 Cuts at: 3585 3633 3683 4425 4510 5581 1391 Size: 48 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 303 221 198 177 99 88 85 63 61 55 50 48 48 20 12 10 8 BspEI T'CCGG_A Cuts at: 2647 6027 2647 Size: 3380 2984 BspHI T'CATG_A Cuts at: 2517 4253 5983 2517 Size: 1736 1730 2898 Fragments arranged by size: 2898 1736 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3398 3719 844 Size: 1376 1178 321 3489 Fragments arranged by size: 3489 1376 1178 321 BsrBI CCG'CTC Cuts at: 1394 1823 2899 2905 2929 2935 2998 3091 3377 Size: 429 1076 6 24 6 63 93 286 Cuts at: 3377 4251 1394 Size: 874 3507 Fragments arranged by size: 3507 1076 874 429 286 93 63 24 6 6 BsrDI GCAATG_nn' Cuts at: 65 1248 3512 4859 5033 65 Size: 1183 2264 1347 174 1396 Fragments arranged by size: 2264 1396 1347 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 2124 2968 3403 3649 5008 5195 Size: 110 251 63 844 435 246 1359 187 Cuts at: 5195 1700 Size: 2869 Fragments arranged by size: 2869 1359 844 435 251 246 187 110 63 BsrGI T'GTAC_A Cuts at: 96 2421 96 Size: 2325 4039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 2425 2779 4424 5436 2425 Size: 354 1645 1012 3353 Fragments arranged by size: 3353 1645 1012 354 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 3118 1184 Size: 1934 4430 BstYI r'GATC_y Cuts at: 1972 2872 2908 3694 4061 4446 4463 5915 6001 Size: 900 36 786 367 385 17 1452 86 Cuts at: 6001 6013 6359 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 900 786 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2665 2665 Size: 6364 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2535 2629 2940 2955 513 Size: 552 939 30 501 94 311 15 3922 Fragments arranged by size: 3922 939 552 501 311 94 30 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2269 487 Size: 683 56 1043 4582 Fragments arranged by size: 4582 1043 683 56 BtsI GCAGTG_nn' Cuts at: 3880 4674 4694 3880 Size: 794 20 5550 Fragments arranged by size: 5550 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2589 2624 529 Size: 35 2025 35 4269 Fragments arranged by size: 4269 2025 35 35 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DraIII CAC_nnn'GTG Cuts at: 2074 2074 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2473 3206 5371 809 Size: 507 1157 733 2165 1802 Fragments arranged by size: 2165 1802 1157 733 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 2578 3011 Size: 54 1425 50 275 18 229 519 433 Cuts at: 3011 3372 3459 3474 3651 3811 4700 6329 8 Size: 361 87 15 177 160 889 1629 43 Fragments arranged by size: 1629 1425 889 519 433 361 275 229 177 160 87 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2059 3372 3459 3811 1537 Size: 275 247 1313 87 352 4090 Fragments arranged by size: 4090 1313 352 275 247 87 EarI CTCTTCn'nnn_ Cuts at: 1353 3371 3605 4294 1353 Size: 2018 234 689 3423 Fragments arranged by size: 3423 2018 689 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 3215 4951 5325 5471 387 Size: 1064 138 451 1175 1736 374 146 1280 Fragments arranged by size: 1736 1280 1175 1064 451 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2623 Size: 6364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2132 2177 2180 2252 2784 2809 Size: 393 455 45 45 3 72 532 25 Cuts at: 2809 2853 3011 3115 3179 3440 3455 3662 4427 Size: 44 158 104 64 261 15 207 765 Cuts at: 4427 5024 5811 6079 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 765 597 532 455 393 268 261 207 158 104 72 64 45 45 44 25 15 3 EcoNI CCTnn'n_nnAGG Cuts at: 3353 3623 3353 Size: 270 6094 EcoO109I rG'GnC_Cy Cuts at: 2264 2669 2670 2764 2765 2837 3187 3501 2264 Size: 405 1 94 1 72 350 314 5127 Fragments arranged by size: 5127 405 350 314 94 72 1 1 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 2068 2502 2598 3100 3166 3569 5511 1678 Size: 390 434 96 502 66 403 1942 2531 Fragments arranged by size: 2531 1942 502 434 403 390 96 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2291 2323 5005 5037 5079 Size: 32 135 32 719 32 2682 32 42 Cuts at: 5079 5111 1373 Size: 32 2626 Fragments arranged by size: 2682 2626 719 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 2064 2064 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2580 3013 3476 10 Size: 54 1425 343 748 433 463 2898 Fragments arranged by size: 2898 1425 748 463 433 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4322 4681 4840 518 Size: 648 3156 359 159 2042 Fragments arranged by size: 3156 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2296 2631 2720 3017 3551 3780 4457 Size: 430 260 335 89 297 534 229 677 Cuts at: 4457 5605 5850 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 430 335 297 260 245 229 89 NaeI GCC'GGC Cuts at: 2063 2126 3405 3651 2063 Size: 63 1279 246 4776 Fragments arranged by size: 4776 1279 246 63 NarI GG'CG_CC Cuts at: 2065 2065 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4873 Fragments arranged by size: 4873 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NgoMIV G'CCGG_C Cuts at: 2061 2124 3403 3649 2061 Size: 63 1279 246 4776 Fragments arranged by size: 4776 1279 246 63 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 3443 3487 3722 5267 1132 Size: 97 2214 44 235 1545 2229 Fragments arranged by size: 2229 2214 1545 235 97 44 PasI CC'CwG_GG Cuts at: 1348 3590 1348 Size: 2242 4122 PciI A'CATG_T Cuts at: 3483 5263 3483 Size: 1780 4584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6364 PfoI T'CCnGG_A Cuts at: 2249 3327 2249 Size: 1078 5286 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PmlI CAC'GTG Cuts at: 2121 2121 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3187 3501 3187 Size: 314 6050 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2669 2764 2774 2669 Size: 95 10 6259 Fragments arranged by size: 6259 95 10 PspXI vC'TCGA_Gb Cuts at: 2308 2308 Size: 6364 PstI C_TGCA'G Cuts at: 830 2391 2475 3006 3716 830 Size: 1561 84 531 710 3478 Fragments arranged by size: 3478 1561 710 531 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2296 2720 3551 2296 Size: 424 831 5109 Fragments arranged by size: 5109 831 424 SacI G_AGCT'C Cuts at: 2625 2625 Size: 6364 SacII CC_GC'GG Cuts at: 2037 2632 2037 Size: 595 5769 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SanDI GG'GwC_CC Cuts at: 3501 3501 Size: 6364 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2387 2471 3002 3212 3712 4847 5528 Size: 219 1342 84 531 210 500 1135 681 Cuts at: 5528 5719 826 Size: 191 1471 Fragments arranged by size: 1471 1342 1135 681 531 500 219 210 191 84 SfiI GGCCn_nnn'nGGCC Cuts at: 2782 2782 Size: 6364 SfoI GGC'GCC Cuts at: 2066 2066 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2308 2638 3080 4468 5369 5631 Size: 197 926 365 330 442 1388 901 262 Cuts at: 5631 820 Size: 1553 Fragments arranged by size: 1553 1388 926 901 442 365 330 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3443 3722 1132 Size: 2311 279 3774 Fragments arranged by size: 3774 2311 279 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 2785 3008 3128 1517 Size: 1268 223 120 4753 Fragments arranged by size: 4753 1268 223 120 StyI C'CwwG_G Cuts at: 513 1065 2004 2607 3114 3123 3803 513 Size: 552 939 603 507 9 680 3074 Fragments arranged by size: 3074 939 680 603 552 507 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4391 4408 4561 4746 5191 325 Size: 1255 2811 17 153 185 445 1498 Fragments arranged by size: 2811 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 4610 6322 Size: 274 80 33 51 1443 444 2189 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 2189 1712 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2105 2137 6324 6356 2105 Size: 32 4187 32 2113 Fragments arranged by size: 4187 2113 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2233 3238 2233 Size: 1005 5359 XhoI C'TCGA_G Cuts at: 1943 2308 1943 Size: 365 5999 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 3365 4493 3365 Size: 1128 5236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PsrI RsrII SapI SexAI SrfI SwaI