(Linear) (Six-base) MAPSORT of: KIEE6424.seq Check: 8695 from: 1 to: 1659 KIEE6424.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1393 1659 Size: 1393 266 AccI GT'mk_AC Cuts at: 0 1409 1659 Size: 1409 250 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 175 779 1110 1657 1659 Size: 82 93 604 331 547 2 Fragments arranged by size: 604 547 331 93 82 2 AfeI AGC'GCT Cuts at: 0 591 1159 1659 Size: 591 568 500 Fragments arranged by size: 591 568 500 AflIII A'CryG_T Cuts at: 0 113 1478 1659 Size: 113 1365 181 Fragments arranged by size: 1365 181 113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 109 301 481 1659 Size: 109 192 180 1178 Fragments arranged by size: 1178 192 180 109 AlwNI CAG_nnn'CTG Cuts at: 0 105 474 1519 1549 1659 Size: 105 369 1045 30 110 Fragments arranged by size: 1045 369 110 105 30 ApaI G_GGCC'C Cuts at: 0 668 763 773 1659 Size: 668 95 10 886 Fragments arranged by size: 886 668 95 10 ApaLI G'TGCA_C Cuts at: 0 1378 1659 Size: 1378 281 AvaI C'yCGr_G Cuts at: 0 303 735 1279 1659 Size: 303 432 544 380 Fragments arranged by size: 544 432 380 303 BamHI G'GATC_C Cuts at: 0 867 903 1659 Size: 867 36 756 Fragments arranged by size: 867 756 36 BanI G'GyrC_C Cuts at: 0 59 234 705 1623 1659 Size: 59 175 471 918 36 Fragments arranged by size: 918 471 175 59 36 BanII G_rGCy'C Cuts at: 0 559 620 668 763 773 950 1580 1659 Size: 559 61 48 95 10 177 630 79 Fragments arranged by size: 630 559 177 95 79 61 48 10 BbeI G_GCGC'C Cuts at: 0 63 1659 Size: 63 1596 BbsI GAAGACnn'nnnn_ Cuts at: 0 1065 1659 Size: 1065 594 BbvCI CC'TCA_GC Cuts at: 0 1550 1659 Size: 1550 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 72 106 1659 Size: 72 34 1553 Fragments arranged by size: 1553 72 34 BglI GCCn_nnn'nGGC Cuts at: 0 777 830 1632 1659 Size: 777 53 802 27 Fragments arranged by size: 802 777 53 27 BlpI GC'TnA_GC Cuts at: 0 619 1659 Size: 619 1040 Bme1580I G_kGCm'C Cuts at: 0 239 460 668 708 763 773 1382 1628 Size: 239 221 208 40 55 10 609 246 Cuts at: 1628 1659 Size: 31 Fragments arranged by size: 609 246 239 221 208 55 40 31 10 BmgBI CAC'GTC Cuts at: 0 1053 1461 1659 Size: 1053 408 198 Fragments arranged by size: 1053 408 198 BmrI ACTGGGnnnn_n' Cuts at: 0 400 1659 Size: 400 1259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 172 247 804 848 1006 1174 1435 Size: 127 45 75 557 44 158 168 261 Cuts at: 1435 1450 1659 Size: 15 209 Fragments arranged by size: 557 261 209 168 158 127 75 45 44 15 Bpu10I CC'TnA_GC Cuts at: 0 1550 1574 1659 Size: 1550 24 85 Fragments arranged by size: 1550 85 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 654 1060 1659 Size: 654 406 599 Fragments arranged by size: 654 599 406 BsaAI yAC'GTr Cuts at: 0 116 1659 Size: 116 1543 BsaHI Gr'CG_yC Cuts at: 0 60 79 1659 Size: 60 19 1580 Fragments arranged by size: 1580 60 19 BsaWI w'CCGG_w Cuts at: 0 642 1659 Size: 642 1017 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 100 130 1659 Size: 100 30 1529 Fragments arranged by size: 1529 100 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 180 1003 1275 1408 1567 1623 1659 Size: 180 823 272 133 159 56 36 Fragments arranged by size: 823 272 180 159 133 56 36 BseYI C'CCAG_C Cuts at: 0 474 872 908 1070 1225 1595 1659 Size: 474 398 36 162 155 370 64 Fragments arranged by size: 474 398 370 162 155 64 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1511 1659 Size: 1511 148 BsiEI CG_ry'CG Cuts at: 0 57 1197 1370 1457 1659 Size: 57 1140 173 87 202 Fragments arranged by size: 1140 202 173 87 57 BsiHKAI G_wGCw'C Cuts at: 0 88 176 620 680 700 1382 1659 Size: 88 88 444 60 20 682 277 Fragments arranged by size: 682 444 277 88 88 60 20 Bsp1286I G_dGCh'C Cuts at: 0 88 176 239 460 559 620 668 680 Size: 88 88 63 221 99 61 48 12 Cuts at: 680 700 708 763 773 950 1382 1580 1628 Size: 20 8 55 10 177 432 198 48 Cuts at: 1628 1659 Size: 31 Fragments arranged by size: 432 221 198 177 99 88 88 63 61 55 48 48 31 20 12 10 8 BspEI T'CCGG_A Cuts at: 0 642 1659 Size: 642 1017 BspHI T'CATG_A Cuts at: 0 512 1659 Size: 512 1147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 215 1393 1659 Size: 215 1178 266 Fragments arranged by size: 1178 266 215 BsrBI CCG'CTC Cuts at: 0 894 900 924 930 993 1086 1372 1659 Size: 894 6 24 6 63 93 286 287 Fragments arranged by size: 894 287 286 93 63 24 6 6 BsrDI GCAATG_nn' Cuts at: 0 1507 1659 Size: 1507 152 BsrFI r'CCGG_y Cuts at: 0 56 119 963 1398 1644 1659 Size: 56 63 844 435 246 15 Fragments arranged by size: 844 435 246 63 56 15 BsrGI T'GTAC_A Cuts at: 0 416 1659 Size: 416 1243 BssSI C'ACGA_G Cuts at: 0 420 774 1659 Size: 420 354 885 Fragments arranged by size: 885 420 354 BstEII G'GTnAC_C Cuts at: 0 1113 1659 Size: 1113 546 BstYI r'GATC_y Cuts at: 0 867 903 1659 Size: 867 36 756 Fragments arranged by size: 867 756 36 Bsu36I CC'TnA_GG Cuts at: 0 660 1659 Size: 660 999 BtgI C'CryG_G Cuts at: 0 29 530 624 935 950 1659 Size: 29 501 94 311 15 709 Fragments arranged by size: 709 501 311 94 29 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 264 1659 Size: 264 1395 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 584 619 1659 Size: 584 35 1040 Fragments arranged by size: 1040 584 35 DraIII CAC_nnn'GTG Cuts at: 0 69 1659 Size: 69 1590 DrdI GACnn_nn'nnGTC Cuts at: 0 468 1201 1659 Size: 468 733 458 Fragments arranged by size: 733 468 458 EaeI y'GGCC_r Cuts at: 0 54 573 1006 1367 1454 1469 1646 1659 Size: 54 519 433 361 87 15 177 13 Fragments arranged by size: 519 433 361 177 87 54 15 13 EagI C'GGCC_G Cuts at: 0 54 1367 1454 1659 Size: 54 1313 87 205 Fragments arranged by size: 1313 205 87 54 EarI CTCTTCn'nnn_ Cuts at: 0 1366 1600 1659 Size: 1366 234 59 Fragments arranged by size: 1366 234 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 35 1210 1659 Size: 35 1175 449 Fragments arranged by size: 1175 449 35 EcoICRI GAG'CTC Cuts at: 0 618 1659 Size: 618 1041 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 127 172 175 247 779 804 848 Size: 82 45 45 3 72 532 25 44 Cuts at: 848 1006 1110 1174 1435 1450 1657 1659 Size: 158 104 64 261 15 207 2 Fragments arranged by size: 532 261 207 158 104 82 72 64 45 45 44 25 15 3 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1348 1618 1659 Size: 1348 270 41 Fragments arranged by size: 1348 270 41 EcoO109I rG'GnC_Cy Cuts at: 0 259 664 665 759 760 832 1182 1496 Size: 259 405 1 94 1 72 350 314 Cuts at: 1496 1659 Size: 163 Fragments arranged by size: 405 350 314 259 163 94 72 1 1 HaeII r_GCGC'y Cuts at: 0 63 497 593 1095 1161 1564 1659 Size: 63 434 96 502 66 403 95 Fragments arranged by size: 502 434 403 96 95 66 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 286 318 1659 Size: 286 32 1341 Fragments arranged by size: 1341 286 32 KasI G'GCGC_C Cuts at: 0 59 1659 Size: 59 1600 MscI TGG'CCA Cuts at: 0 575 1008 1471 1659 Size: 575 433 463 188 Fragments arranged by size: 575 463 433 188 MspA1I CmG'CkG Cuts at: 0 31 291 626 715 1012 1546 1659 Size: 31 260 335 89 297 534 113 Fragments arranged by size: 534 335 297 260 113 89 31 NaeI GCC'GGC Cuts at: 0 58 121 1400 1646 1659 Size: 58 63 1279 246 13 Fragments arranged by size: 1279 246 63 58 13 NarI GG'CG_CC Cuts at: 0 60 1659 Size: 60 1599 NgoMIV G'CCGG_C Cuts at: 0 56 119 1398 1644 1659 Size: 56 63 1279 246 15 Fragments arranged by size: 1279 246 63 56 15 NspI r_CATG'y Cuts at: 0 1438 1482 1659 Size: 1438 44 177 Fragments arranged by size: 1438 177 44 PasI CC'CwG_GG Cuts at: 0 1585 1659 Size: 1585 74 PciI A'CATG_T Cuts at: 0 1478 1659 Size: 1478 181 PfoI T'CCnGG_A Cuts at: 0 244 1322 1659 Size: 244 1078 337 Fragments arranged by size: 1078 337 244 PmlI CAC'GTG Cuts at: 0 116 1659 Size: 116 1543 PpuMI rG'GwC_Cy Cuts at: 0 1182 1496 1659 Size: 1182 314 163 Fragments arranged by size: 1182 314 163 PspOMI G'GGCC_C Cuts at: 0 664 759 769 1659 Size: 664 95 10 890 Fragments arranged by size: 890 664 95 10 PspXI vC'TCGA_Gb Cuts at: 0 303 1659 Size: 303 1356 PstI C_TGCA'G Cuts at: 0 386 470 1001 1659 Size: 386 84 531 658 Fragments arranged by size: 658 531 386 84 PvuII CAG'CTG Cuts at: 0 291 715 1546 1659 Size: 291 424 831 113 Fragments arranged by size: 831 424 291 113 SacI G_AGCT'C Cuts at: 0 620 1659 Size: 620 1039 SacII CC_GC'GG Cuts at: 0 32 627 1659 Size: 32 595 1032 Fragments arranged by size: 1032 595 32 SanDI GG'GwC_CC Cuts at: 0 1496 1659 Size: 1496 163 SfcI C'TryA_G Cuts at: 0 382 466 997 1207 1659 Size: 382 84 531 210 452 Fragments arranged by size: 531 452 382 210 84 SfiI GGCCn_nnn'nGGCC Cuts at: 0 777 1659 Size: 777 882 SfoI GGC'GCC Cuts at: 0 61 1659 Size: 61 1598 SmlI C'TyrA_G Cuts at: 0 303 633 1075 1659 Size: 303 330 442 584 Fragments arranged by size: 584 442 330 303 SphI G_CATG'C Cuts at: 0 1438 1659 Size: 1438 221 StuI AGG'CCT Cuts at: 0 780 1003 1123 1659 Size: 780 223 120 536 Fragments arranged by size: 780 536 223 120 StyI C'CwwG_G Cuts at: 0 602 1109 1118 1659 Size: 602 507 9 541 Fragments arranged by size: 602 541 507 9 TatI w'GTAC_w Cuts at: 0 416 1659 Size: 416 1243 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 100 132 1659 Size: 100 32 1527 Fragments arranged by size: 1527 100 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 228 1233 1659 Size: 228 1005 426 Fragments arranged by size: 1005 426 228 XhoI C'TCGA_G Cuts at: 0 303 1659 Size: 303 1356 XmnI GAAnn'nnTTC Cuts at: 0 1360 1659 Size: 1360 299 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtgZI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SfcI SfiI SfoI SmlI SphI StuI StyI TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BplI BsaI BsaBI BsiWI BsmI BsmBI BssHII BstAPI BstBI BstXI BstZ17I BtsI ClaI DraI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XmaI ZraI