(Circular) (Six-base) MAPSORT of: pFN21AB6254.seq Check: 9911 from: 1 to: 6754 pFN21AB6254 6754 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2697 2740 3490 2697 Size: 43 750 5961 Fragments arranged by size: 5961 750 43 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4564 278 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 3594 4075 3594 Size: 481 6273 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2768 4817 6201 6469 1632 Size: 1136 2049 1384 268 1917 Fragments arranged by size: 2049 1917 1384 1136 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 2238 5585 2238 Size: 3347 3407 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2735 1184 Size: 1551 5203 AlwNI CAG_nnn'CTG Cuts at: 2850 3404 3482 3575 4497 6069 2850 Size: 554 78 93 922 1572 3535 Fragments arranged by size: 3535 1572 922 554 93 78 ApaI G_GGCC'C Cuts at: 2116 3018 3286 2116 Size: 902 268 5584 Fragments arranged by size: 5584 902 268 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 2963 4063 4289 4441 6550 1493 Size: 1470 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1470 1100 226 152 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2178 2246 2255 2836 3000 3009 Size: 73 129 106 68 9 581 164 9 Cuts at: 3009 3089 3286 3900 4079 1870 Size: 80 197 614 179 4545 Fragments arranged by size: 4545 614 581 197 179 164 129 106 80 73 68 9 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2875 2908 2875 Size: 33 6721 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2251 2471 2685 3169 3213 Size: 325 217 627 464 220 214 484 44 Cuts at: 3213 3594 3850 4075 5530 618 Size: 381 256 225 1455 1842 Fragments arranged by size: 1842 1455 627 484 464 381 325 256 225 220 217 214 44 BanII G_rGCy'C Cuts at: 1391 2116 2455 2517 3009 3018 3286 3318 3424 Size: 725 339 62 492 9 268 32 106 Cuts at: 3424 3453 4073 1391 Size: 29 620 4072 Fragments arranged by size: 4072 725 620 492 339 268 106 62 32 29 9 BbeI G_GCGC'C Cuts at: 3217 3217 Size: 6754 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3988 928 Size: 971 2089 3694 Fragments arranged by size: 3694 2089 971 BbvCI CC'TCA_GC Cuts at: 2366 2366 Size: 6754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2050 2084 4945 4979 1903 Size: 34 113 34 2861 34 3678 Fragments arranged by size: 3678 2861 113 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4646 5856 4646 Size: 1210 5544 BclI T'GATC_A Cuts at: 1548 1548 Size: 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2021 3013 Size: 107 122 71 631 605 337 12 992 Cuts at: 3013 3800 3964 5365 136 Size: 787 164 1401 1525 Fragments arranged by size: 1525 1401 992 787 631 605 337 164 122 107 71 12 BglII A'GATC_T Cuts at: 6749 6749 Size: 6754 BlpI GC'TnA_GC Cuts at: 3040 4171 3040 Size: 1131 5623 Bme1580I G_kGCm'C Cuts at: 1790 2073 2116 2256 3018 3172 3286 3753 3768 Size: 283 43 140 762 154 114 467 15 Cuts at: 3768 3796 3896 4815 5971 1790 Size: 28 100 919 1156 2573 Fragments arranged by size: 2573 1156 919 762 467 283 154 140 114 100 43 28 15 BmgBI CAC'GTC Cuts at: 1318 2500 1318 Size: 1182 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 4485 5443 Size: 416 53 83 399 24 1240 1828 958 Cuts at: 5443 442 Size: 1753 Fragments arranged by size: 1828 1753 1240 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6754 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3055 Size: 6754 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2064 2303 3872 3941 5414 1239 Size: 825 239 1569 69 1473 2579 Fragments arranged by size: 2579 1569 1473 825 239 69 Bpu10I CC'TnA_GC Cuts at: 2291 2366 3317 3553 2291 Size: 75 951 236 5492 Fragments arranged by size: 5492 951 236 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3339 3668 3782 4879 5744 6042 3339 Size: 329 114 1097 865 298 4051 Fragments arranged by size: 4051 1097 865 329 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 3045 3505 3577 5417 882 Size: 617 583 963 460 72 1840 2219 Fragments arranged by size: 2219 1840 963 617 583 460 72 BsaAI yAC'GTr Cuts at: 493 1204 4003 4545 493 Size: 711 2799 542 2702 Fragments arranged by size: 2799 2702 711 542 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3214 4561 4943 5578 Size: 53 83 186 936 1681 1347 382 635 Cuts at: 5578 275 Size: 1451 Fragments arranged by size: 1681 1451 1347 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2238 2571 2982 5187 5585 5859 6006 6417 2238 Size: 333 411 2205 398 274 147 411 2575 Fragments arranged by size: 2575 2205 411 411 398 333 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2086 2116 2636 2666 3042 Size: 30 165 30 683 30 520 30 376 Cuts at: 3042 3072 1178 Size: 30 4860 Fragments arranged by size: 4860 683 520 376 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2135 3134 3203 3218 3529 3875 1186 Size: 874 75 999 69 15 311 346 4065 Fragments arranged by size: 4065 999 874 346 311 75 69 15 BseYI C'CCAG_C Cuts at: 2326 2446 2605 5957 6453 2326 Size: 120 159 3352 496 2627 Fragments arranged by size: 3352 2627 496 159 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 3080 1163 Size: 573 388 956 4837 Fragments arranged by size: 4837 956 573 388 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3740 4204 4965 5993 1540 Size: 275 186 15 1724 464 761 1028 2301 Fragments arranged by size: 2301 1724 1028 761 464 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2517 3872 4815 4900 5971 2517 Size: 1355 943 85 1071 3300 Fragments arranged by size: 3300 1355 1071 943 85 BsmI GAATG_Cn' Cuts at: 4265 4358 4265 Size: 93 6661 BsmBI CGTCTCn'nnnn_ Cuts at: 2089 2656 2089 Size: 567 6187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2116 2256 2455 2517 3009 3018 Size: 399 283 43 140 199 62 492 9 Cuts at: 3018 3172 3286 3318 3424 3453 3753 3768 3796 Size: 154 114 32 106 29 300 15 28 Cuts at: 3796 3872 3896 4073 4815 4900 5971 1391 Size: 76 24 177 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 300 283 199 177 154 140 114 106 85 76 62 43 32 29 28 24 15 9 BspEI T'CCGG_A Cuts at: 2571 2982 6417 2571 Size: 411 3435 2908 Fragments arranged by size: 3435 2908 411 BspHI T'CATG_A Cuts at: 4643 6373 4643 Size: 1730 5024 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2683 2697 2740 3490 4109 844 Size: 1839 14 43 750 619 3489 Fragments arranged by size: 3489 1839 750 619 43 14 BsrBI CCG'CTC Cuts at: 1394 1823 2110 3208 3244 3270 4641 1394 Size: 429 287 1098 36 26 1371 3507 Fragments arranged by size: 3507 1371 1098 429 287 36 26 BsrDI GCAATG_nn' Cuts at: 65 1248 5249 5423 65 Size: 1183 4001 174 1396 Fragments arranged by size: 4001 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 2238 3193 5398 5585 1700 Size: 110 347 81 955 2205 187 2869 Fragments arranged by size: 2869 2205 955 347 187 110 81 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6754 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 4814 5826 4814 Size: 1012 5742 BstAPI GCAn_nnn'nTGC Cuts at: 794 3404 794 Size: 2610 4144 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstYI r'GATC_y Cuts at: 1972 2863 2985 3880 4084 4451 4836 4853 6305 Size: 891 122 895 204 367 385 17 1452 Cuts at: 6305 6391 6403 6749 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 895 891 385 367 346 204 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2417 2681 3675 3855 2417 Size: 264 994 180 5316 Fragments arranged by size: 5316 994 264 180 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 3488 513 Size: 552 939 123 1361 3779 Fragments arranged by size: 3779 1361 939 552 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6015 Fragments arranged by size: 6015 683 56 BtsI GCAGTG_nn' Cuts at: 2107 3894 4270 5064 5084 2107 Size: 1787 376 794 20 3777 Fragments arranged by size: 3777 1787 794 376 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6719 DraI TTT'AAA Cuts at: 4058 4058 Size: 6754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2567 3083 5761 809 Size: 507 1251 516 2678 1802 Fragments arranged by size: 2678 1802 1251 516 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2130 3491 Size: 54 1425 50 275 18 183 117 1361 Cuts at: 3491 3684 3696 3723 3737 4201 5090 6719 8 Size: 193 12 27 14 464 889 1629 43 Fragments arranged by size: 1629 1425 1361 889 464 275 193 183 117 54 50 43 27 18 14 12 EagI C'GGCC_G Cuts at: 1537 1812 2013 3737 4201 1537 Size: 275 201 1724 464 4090 Fragments arranged by size: 4090 1724 464 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2554 2595 3096 3533 4684 1353 Size: 1201 41 501 437 1151 3423 Fragments arranged by size: 3423 1201 1151 501 437 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2615 5341 5715 5861 387 Size: 1064 138 1026 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 1026 374 146 138 EcoICRI GAG'CTC Cuts at: 2515 2515 Size: 6754 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2303 2768 3872 3941 4817 5414 Size: 393 432 239 465 1104 69 876 597 Cuts at: 5414 6201 6469 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1104 876 787 597 465 432 393 268 239 69 EcoNI CCTnn'n_nnAGG Cuts at: 2120 3150 3674 3686 2120 Size: 1030 524 12 5188 Fragments arranged by size: 5188 1030 524 12 EcoO109I rG'GnC_Cy Cuts at: 2113 2139 2287 2422 2560 2611 2815 3014 3282 Size: 26 148 135 138 51 204 199 268 Cuts at: 3282 3283 3557 3643 3679 3949 2113 Size: 1 274 86 36 270 4918 Fragments arranged by size: 4918 274 270 268 204 199 148 138 135 86 51 36 26 1 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3470 3470 Size: 6754 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 2164 3217 5901 1678 Size: 486 1053 2684 2531 Fragments arranged by size: 2684 2531 1053 486 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2504 2536 2830 2862 3854 Size: 32 135 32 932 32 294 32 992 Cuts at: 3854 3886 5395 5427 5469 5501 1373 Size: 32 1509 32 42 32 2626 Fragments arranged by size: 2626 1509 992 932 294 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 4098 4344 669 Size: 988 281 1017 1143 246 3079 Fragments arranged by size: 3079 1143 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6754 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KasI G'GCGC_C Cuts at: 3213 3213 Size: 6754 KpnI G_GTAC'C Cuts at: 3598 4079 3598 Size: 481 6273 MfeI C'AATT_G Cuts at: 2305 4353 2305 Size: 2048 4706 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5868 6052 1305 Size: 4563 184 2007 Fragments arranged by size: 4563 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 3698 3725 10 Size: 54 1425 343 300 1566 27 3039 Fragments arranged by size: 3039 1566 1425 343 300 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 3761 4712 5071 5230 518 Size: 648 2595 951 359 159 2042 Fragments arranged by size: 2595 2042 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2380 2410 3044 3232 3410 3634 3664 Size: 666 108 30 634 188 178 224 30 Cuts at: 3664 3721 4170 4847 5995 6240 1606 Size: 57 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 634 449 245 224 188 178 108 57 30 30 NaeI GCC'GGC Cuts at: 3195 3195 Size: 6754 NarI GG'CG_CC Cuts at: 3214 3214 Size: 6754 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5263 Fragments arranged by size: 5263 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NgoMIV G'CCGG_C Cuts at: 3193 3193 Size: 6754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6754 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 4112 5657 1132 Size: 97 2883 1545 2229 Fragments arranged by size: 2883 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2476 2809 3146 1348 Size: 1128 333 337 4956 Fragments arranged by size: 4956 1128 337 333 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6754 PfoI T'CCnGG_A Cuts at: 2231 2872 2231 Size: 641 6113 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2139 2422 2560 2611 2815 3643 3679 2139 Size: 283 138 51 204 828 36 5214 Fragments arranged by size: 5214 828 283 204 138 51 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PspOMI G'GGCC_C Cuts at: 2112 3014 3282 2112 Size: 902 268 5584 Fragments arranged by size: 5584 902 268 PstI C_TGCA'G Cuts at: 830 2694 2724 2860 3409 3663 4106 830 Size: 1864 30 136 549 254 443 3478 Fragments arranged by size: 3478 1864 549 443 254 136 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2272 2380 2410 3232 3634 3721 2272 Size: 108 30 822 402 87 5305 Fragments arranged by size: 5305 822 402 108 87 30 RsrII CG'GwC_CG Cuts at: 3912 3912 Size: 6754 SacI G_AGCT'C Cuts at: 2517 2517 Size: 6754 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SanDI GG'GwC_CC Cuts at: 2139 2139 Size: 6754 SapI GCTCTTCn'nnn_ Cuts at: 2595 3096 2595 Size: 501 6253 SbfI CC_TGCA'GG Cuts at: 2694 4106 2694 Size: 1412 5342 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2690 2720 2856 3405 3659 4102 5237 Size: 219 1645 30 136 549 254 443 1135 Cuts at: 5237 5918 6109 826 Size: 681 191 1471 Fragments arranged by size: 1645 1471 1135 681 549 443 254 219 191 136 30 SfoI GGC'GCC Cuts at: 3215 3215 Size: 6754 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 2074 2180 3002 3011 3091 3902 4081 1872 Size: 202 106 822 9 80 811 179 4545 Fragments arranged by size: 4545 822 811 202 179 106 80 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 3354 3647 3797 4858 5759 6021 Size: 197 926 1411 293 150 1061 901 262 Cuts at: 6021 820 Size: 1553 Fragments arranged by size: 1553 1411 1061 926 901 293 262 197 150 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6754 SphI G_CATG'C Cuts at: 1132 4112 1132 Size: 2980 3774 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6754 StyI C'CwwG_G Cuts at: 513 1065 2004 2331 4193 513 Size: 552 939 327 1862 3074 Fragments arranged by size: 3074 1862 939 552 327 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 4781 4798 4951 5136 5581 325 Size: 1255 651 2550 17 153 185 445 1498 Fragments arranged by size: 2550 1498 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5000 6712 96 Size: 274 80 33 51 1443 3023 1712 138 Fragments arranged by size: 3023 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2086 2118 3042 3074 6714 6746 2086 Size: 32 924 32 3640 32 2094 Fragments arranged by size: 3640 2094 924 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3575 1316 Size: 2259 4495 XbaI T'CTAG_A Cuts at: 3826 4090 3826 Size: 264 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 2072 2178 3000 3009 3089 3900 4079 1870 Size: 202 106 822 9 80 811 179 4545 Fragments arranged by size: 4545 822 811 202 179 106 80 9 XmnI GAAnn'nnTTC Cuts at: 2171 4883 2171 Size: 2712 4042 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4562 276 Size: 53 83 186 936 3028 2468 Fragments arranged by size: 3028 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacII SexAI SfiI SrfI SwaI XcmI