################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 140 GGTACC G^GTACC AfaI 141 GTAC GT^AC AlwI 334 GGATC GGATC(4/5) AocI 352 CCTGAGG CC^T[ACGT]AGG ApaI 323 GGGCCC GGGCC^C Asp718I 140 GGTACC G^GTACC AspHI 25 GAGCAC G[AT]GC[AT]^C AvaI 473 CCCGAG C^[CT]CG[GA]G BanI 140 GGTACC G^G[CT][GA]CC BbsI 59 GTCTTC GAAGAC(2/6) BciVI 391 GGATAC GTATCC(6/5) BglI 73 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 59 GTCTTC GAAGAC(2/6) BsaI 13 GAGACC GGTCTC(1/5) BseRI 567 CTCCTC GAGGAG(10/8) BsiHKAI 25 GAGCAC G[AT]GC[AT]^C BsoBI 473 CCCGAG C^[CT]CG[GA]G BspMI 434 ACCTGC ACCTGC(4/8) Bsu36I 352 CCTGAGG CC^T[ACGT]AGG CfoI 212 GCGC GCG^C DsaI 417 CCACGG C^C[GA][CT]GG EaeI 313 CGGCCA [CT]^GGCC[GA] Eco81I 352 CCTGAGG CC^T[ACGT]AGG HaeII 211 GGCGCT [GA]GCGC^[CT] HhaI 212 GCGC GCG^C HinP1I 212 GCGC G^CGC KpnI 140 GGTACC GGTAC^C MboII 60 TCTTC GAAGA(8/7) NlaIII 585 CATG CATG^ PshAI 97 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 244 CAGCTG CAG^CTG RsaI 141 GTAC GT^AC SfaNI 157 GATGC GCATC(5/9) SmlI 382 CTTGAG C^T[CT][GA]AG TaqI 23 TCGA T^CGA TfiI 536 GAATC G^A[AT]TC Tsp509I 116 AATT ^AATT TthHB8I 23 TCGA T^CGA XcmI 309 CCAGCGGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BpmI 99 CTCCAG CTGGAG(16/14) 181 CTGGAG BsgI 430 CTGCAC GTGCAG(16/14) 467 CTGCAC BsmAI 13 GAGAC GTCTC(1/5) 236 GTCTC BsrI 101 CCAGT ACTGG(1/-1) 405 CCAGT DpnII 186 GATC ^GATC 335 GATC FauI 426 CCCGC CCCGC(4/6) 514 CCCGC MboI 186 GATC ^GATC 335 GATC NdeII 186 GATC ^GATC 335 GATC PpuMI 461 GGGACCC [GA]G^G[AT]CC[CT] 591 GGGACCT PstI 241 CTGCAG CTGCA^G 360 CTGCAG Sau3AI 186 GATC ^GATC 335 GATC SfcI 241 CTGCAG C^T[GA][CT]AG 360 CTGCAG StuI 356 AGGCCT AGG^CCT 504 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIV PleI Sau96I ScrFI TseI