Restriction map for
pFN21AE1420
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tru9I Tsp45I TspRI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 140 GGTACC G^GTACC
AfaI 141 GTAC GT^AC
AlwI 334 GGATC GGATC(4/5)
AocI 352 CCTGAGG CC^T[ACGT]AGG
ApaI 323 GGGCCC GGGCC^C
Asp718I 140 GGTACC G^GTACC
AspHI 25 GAGCAC G[AT]GC[AT]^C
AvaI 473 CCCGAG C^[CT]CG[GA]G
BanI 140 GGTACC G^G[CT][GA]CC
BbsI 59 GTCTTC GAAGAC(2/6)
BciVI 391 GGATAC GTATCC(6/5)
BglI 73 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 59 GTCTTC GAAGAC(2/6)
BsaI 13 GAGACC GGTCTC(1/5)
BseRI 567 CTCCTC GAGGAG(10/8)
BsiHKAI 25 GAGCAC G[AT]GC[AT]^C
BsoBI 473 CCCGAG C^[CT]CG[GA]G
BspMI 434 ACCTGC ACCTGC(4/8)
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
CfoI 212 GCGC GCG^C
DsaI 417 CCACGG C^C[GA][CT]GG
EaeI 313 CGGCCA [CT]^GGCC[GA]
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
HaeII 211 GGCGCT [GA]GCGC^[CT]
HhaI 212 GCGC GCG^C
HinP1I 212 GCGC G^CGC
KpnI 140 GGTACC GGTAC^C
MboII 60 TCTTC GAAGA(8/7)
NlaIII 585 CATG CATG^
PshAI 97 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 244 CAGCTG CAG^CTG
RsaI 141 GTAC GT^AC
SfaNI 157 GATGC GCATC(5/9)
SmlI 382 CTTGAG C^T[CT][GA]AG
TaqI 23 TCGA T^CGA
TfiI 536 GAATC G^A[AT]TC
Tsp509I 116 AATT ^AATT
TthHB8I 23 TCGA T^CGA
XcmI 309 CCAGCGGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BpmI 99 CTCCAG CTGGAG(16/14)
181 CTGGAG
BsgI 430 CTGCAC GTGCAG(16/14)
467 CTGCAC
BsmAI 13 GAGAC GTCTC(1/5)
236 GTCTC
BsrI 101 CCAGT ACTGG(1/-1)
405 CCAGT
DpnII 186 GATC ^GATC
335 GATC
FauI 426 CCCGC CCCGC(4/6)
514 CCCGC
MboI 186 GATC ^GATC
335 GATC
NdeII 186 GATC ^GATC
335 GATC
PpuMI 461 GGGACCC [GA]G^G[AT]CC[CT]
591 GGGACCT
PstI 241 CTGCAG CTGCA^G
360 CTGCAG
Sau3AI 186 GATC ^GATC
335 GATC
SfcI 241 CTGCAG C^T[GA][CT]AG
360 CTGCAG
StuI 356 AGGCCT AGG^CCT
504 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
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AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
PleI Sau96I ScrFI TseI