Restriction map for pFN21AE1420
 (Circular) (Six-base) MAPSORT of: pfn21a_k1.seq  Check: 6820  from: 1  to: 5064



pFN21A_K1

 With 182 enzymes: SgfI * 

                        June 23, 2008 09:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2164    2874     278 
   Size:         53      83     186     936     628     710    2468

  Fragments arranged by size: 

                2468     936     710     628     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2407    1937 
   Size:        470    4594
 
Acc65I G'GTAC_C

Cuts at:   2385    2385 
   Size:       5064
 
AclI AA'CG_TT

Cuts at:   1563    3191    3564    1563 
   Size:       1628     373    3063

  Fragments arranged by size: 

                3063    1628     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2055    2258    3127    4511    4779    1632 
   Size:        423     203     869    1384     268    1917

  Fragments arranged by size: 

                1917    1384     869     423     268     203
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4867
 
AflIII A'CryG_T

Cuts at:   1203    2044    3963    4940    1203 
   Size:        841    1919     977    1327

  Fragments arranged by size: 

                1919    1327     977     841
 
AgeI A'CCGG_T

Cuts at:   3895    3895 
   Size:       5064
 
AhdI GACnn_n'nnGTC

Cuts at:   3793    3793 
   Size:       5064
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5064
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5064
 
AlwNI CAG_nnn'CTG

Cuts at:   2807    4379    2807 
   Size:       1572    3492
 
ApaLI G'TGCA_C

Cuts at:   3121    4277    3121 
   Size:       1156    3908
 
ApoI r'AATT_y

Cuts at:   1493    2279    2373    2599    2751    4860    1493 
   Size:        786      94     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     786     226     152      94
 
AseI AT'TA_AT

Cuts at:    160    1032    3618     160 
   Size:        872    2586    1606

  Fragments arranged by size: 

                2586    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5064
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2219    2389    1870 
   Size:         73     276     170    4545

  Fragments arranged by size: 

                4545     276     170      73
 
BamHI G'GATC_C

Cuts at:   2394    2761    2394 
   Size:        367    4697
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2385    3840     618 
   Size:        325     217     627     598    1455    1842

  Fragments arranged by size: 

                1842    1455     627     598     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2383    1391 
   Size:        992    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4093
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3255    3289    1903 
   Size:         34    1318      34    3678

  Fragments arranged by size: 

                3678    1318      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2956    4166    2956 
   Size:       1210    3854
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5064
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3675     136 
   Size:        107     122      71     631     605    2003    1525

  Fragments arranged by size: 

                2003    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5059    5059 
   Size:       5064
 
BlpI GC'TnA_GC

Cuts at:   2481    2481 
   Size:       5064
 
Bme1580I G_kGCm'C

Cuts at:   1790    3125    4281    1790 
   Size:       1335    1156    2573

  Fragments arranged by size: 

                2573    1335    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5064
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2795    3753     442 
   Size:        416      53      83     399      24    1378     958    1753

  Fragments arranged by size: 

                1753    1378     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5064
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3724    1239 
   Size:       2485    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2285    3189    4054    4352    2285 
   Size:        904     865     298    2997

  Fragments arranged by size: 

                2997     904     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3727     882 
   Size:        617    2228    2219

  Fragments arranged by size: 

                2228    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2855     493 
   Size:        711    1651    2702

  Fragments arranged by size: 

                2702    1651     711
 
BsaBI GATnn'nnATC

Cuts at:   5058    5058 
   Size:       5064
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2161    2871    3253    3888 
   Size:         53      83     186     936     628     710     382     635

Cuts at:   3888     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     710     635     628     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2287    3497    3895    4169    4316    4727    2287 
   Size:       1210     398     274     147     411    2624

  Fragments arranged by size: 

                2624    1210     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4839

  Fragments arranged by size: 

                4839     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5064
 
BseYI C'CCAG_C

Cuts at:   2131    4267    4763    2131 
   Size:       2136     496    2432

  Fragments arranged by size: 

                2432    2136     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4491
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2514    3275    4303    1540 
   Size:        275     186     513     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     513     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3125    3210    4281    3125 
   Size:         85    1071    3908

  Fragments arranged by size: 

                3908    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2575    2668    2575 
   Size:         93    4971
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2383    3125    3210    4281    1391 
   Size:        399     593     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     593     399      85
 
BspEI T'CCGG_A

Cuts at:   4727    4727 
   Size:       5064
 
BspHI T'CATG_A

Cuts at:   2953    4683    2953 
   Size:       1730    3334
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2419     844 
   Size:       1575    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2951    1394 
   Size:        429    1128    3507

  Fragments arranged by size: 

                3507    1128     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3559    3733      65 
   Size:       1183    2311     174    1396

  Fragments arranged by size: 

                2311    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3708    3895    1700 
   Size:        110    1898     187    2869

  Fragments arranged by size: 

                2869    1898     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5064
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5064
 
BssSI C'ACGA_G

Cuts at:   3124    4136    3124 
   Size:       1012    4052
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5064
 
BstBI TT'CG_AA

Cuts at:   3868    3868 
   Size:       5064
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5064
 
BstYI r'GATC_y

Cuts at:   1972    2394    2761    3146    3163    4615    4701    4713    5059 
   Size:        422     367     385      17    1452      86      12     346

Cuts at:   5059    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     422     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3573

  Fragments arranged by size: 

                3573     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4325

  Fragments arranged by size: 

                4325     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2580    3374    3394    2580 
   Size:        794      20    4250

  Fragments arranged by size: 

                4250     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5064
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5029
 
DraI TTT'AAA

Cuts at:   2368    2368 
   Size:       5064
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4071     809 
   Size:        507    2755    1802

  Fragments arranged by size: 

                2755    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2511    3400    5029 
   Size:         54    1425      50     275      18     681     889    1629

Cuts at:   5029       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     681     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2511    1537 
   Size:        275     699    4090

  Fragments arranged by size: 

                4090     699     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2994    1353 
   Size:       1641    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2200    3651    4025    4171     387 
   Size:       1064     138     611    1451     374     146    1280

  Fragments arranged by size: 

                1451    1280    1064     611     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2055    2258    3127    3724    4511    4779    1239 
   Size:        393     423     203     869     597     787     268    1524

  Fragments arranged by size: 

                1524     869     787     597     423     393     268     203
            
 
EcoRI G'AATT_C

Cuts at:   2373    2751    2373 
   Size:        378    4686
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2169    2169 
   Size:       5064
 
FspI TGC'GCA

Cuts at:   3570    3570 
   Size:       5064
 
HaeII r_GCGC'y

Cuts at:   1678    4211    1678 
   Size:       2533    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2181    2213    3705    3737    3779 
   Size:         32     135      32     609      32    1492      32      42

Cuts at:   3779    3811    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1492     609     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2408    2654     669 
   Size:        988     281     470     246    3079

  Fragments arranged by size: 

                3079     988     470     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5064
 
HpaI GTT'AAC

Cuts at:   2654    2654 
   Size:       5064
 
KpnI G_GTAC'C

Cuts at:   2389    2389 
   Size:       5064
 
MfeI C'AATT_G

Cuts at:   2663    2663 
   Size:       5064
 
MluI A'CGCG_T

Cuts at:   4940    4940 
   Size:       5064
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4178    4362    1305 
   Size:       2873     184    2007

  Fragments arranged by size: 

                2873    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3242

  Fragments arranged by size: 

                3242    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3022    3381    3540     518 
   Size:        648    1856     359     159    2042

  Fragments arranged by size: 

                2042    1856     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2480    3157    4305    4550    1606 
   Size:        874     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     874     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3573

  Fragments arranged by size: 

                3573     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5064
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5064
 
NotI GC'GGCC_GC

Cuts at:   2511    2511 
   Size:       5064
 
NspI r_CATG'y

Cuts at:   1132    1229    2422    3967    1132 
   Size:         97    1193    1545    2229

  Fragments arranged by size: 

                2229    1545    1193      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5064
 
PciI A'CATG_T

Cuts at:   3963    3963 
   Size:       5064
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5064
 
PmeI GTTT'AAAC

Cuts at:   2368    2368 
   Size:       5064
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3133    3165    4646    4678    3133 
   Size:         32    1481      32    3519

  Fragments arranged by size: 

                3519    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5064
 
PsiI TTA'TAA

Cuts at:   2634    2634 
   Size:       5064
 
PstI C_TGCA'G

Cuts at:    830    2416     830 
   Size:       1586    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5064
 
SalI G'TCGA_C

Cuts at:   1936    2406    1936 
   Size:        470    4594
 
SbfI CC_TGCA'GG

Cuts at:   2416    2416 
   Size:       5064
 
ScaI AGT'ACT

Cuts at:   3312    5024    3312 
   Size:       1712    3352
 
SfcI C'TryA_G

Cuts at:    826    1045    2412    3547    4228    4419     826 
   Size:        219    1367    1135     681     191    1471

  Fragments arranged by size: 

                1471    1367    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5064
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5064
 
SmaI CCC'GGG

Cuts at:   1872    2221    2391    1872 
   Size:        349     170    4545

  Fragments arranged by size: 

                4545     349     170
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2300    3168    4069    4331     820 
   Size:        197     926     357     868     901     262    1553

  Fragments arranged by size: 

                1553     926     901     868     357     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2855     493 
   Size:       2362    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5064
 
SphI G_CATG'C

Cuts at:   1132    2422    1132 
   Size:       1290    3774
 
SspI AAT'ATT

Cuts at:      5      52    2988       5 
   Size:         47    2936    2081

  Fragments arranged by size: 

                2936    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5064
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2503     513 
   Size:        552     939     499    3074

  Fragments arranged by size: 

                3074     939     552     499
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2123    3091    3108    3261    3446    3891     325 
   Size:       1255     543     968      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     968     543     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3310    5022      96 
   Size:        274      80      33      51    1443    1333    1712     138

  Fragments arranged by size: 

                1712    1443    1333     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5024    5056    5024 
   Size:         32    5032
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5064
 
XbaI T'CTAG_A

Cuts at:   2400    2400 
   Size:       5064
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5064
 
XmaI C'CCGG_G

Cuts at:   1870    2219    2389    1870 
   Size:        349     170    4545

  Fragments arranged by size: 

                4545     349     170
 
XmnI GAAnn'nnTTC

Cuts at:   3193    3193 
   Size:       5064
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2162    2872     276 
   Size:         53      83     186     936     628     710    2468

  Fragments arranged by size: 

                2468     936     710     628     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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