################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MboI MflI MluI MluNI MscI NarI NciI NcoI NdeI NdeII NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 144 CGCG CG^CG AccIII 28 TCCGGA T^CCGGA AflIII 135 ACGTGT A^C[GA][CT]GT AhdI 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 375 GAAGAC GAAGAC(2/6) BbvI 166 GCAGC GCAGC(8/12) BfaI 116 CTAG C^TAG BpmI 175 CTGGAG CTGGAG(16/14) BpuAI 375 GAAGAC GAAGAC(2/6) BsaWI 28 TCCGGA [AT]^CCGG[AT] BseAI 28 TCCGGA T^CCGGA Bsp1407I 158 TGTACA T^GTACA BspEI 28 TCCGGA T^CCGGA BsrBI 57 CCGCTC CCGCTC(-3/-3) BsrFI 70 GCCGGC [GA]^CCGG[CT] BsrGI 158 TGTACA T^GTACA BssKI 174 CCTGG ^CC[ACGT]GG BstAPI 208 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 174 CCTGG CC^[AT]GG BstUI 144 CGCG CG^CG Cfr10I 70 GCCGGC [GA]^CCGG[CT] Cfr13I 90 GGGCC G^G[ACGT]CC Eam1105I 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 174 CCTGG ^CC[AT]GG EcoT22I 215 ATGCAT ATGCA^T FauI 56 CCCGC CCCGC(4/6) HphI 185 TCACC GGTGA(8/7) MaeI 116 CTAG C^TAG MaeII 135 ACGT A^CGT MaeIII 254 GTCAC ^GT[ACGT]AC MfeI 311 CAATTG C^AATTG MroI 28 TCCGGA T^CCGGA MseI 229 TTAA T^TAA MslI 130 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 152 CCGCTG C[AC]G^C[GT]G MunI 311 CAATTG C^AATTG MvaI 174 CCTGG CC^[AT]GG MvnI 144 CGCG CG^CG NaeI 70 GCCGGC GCC^GGC NgoMIV 70 GCCGGC G^CCGGC NlaIII 130 CATG CATG^ NsiI 215 ATGCAT ATGCA^T PleI 25 GACTC GAGTC(4/5) Sau96I 90 GGGCC G^G[ACGT]CC ScrFI 174 CCTGG CC^[ACGT]GG SfaNI 217 GCATC GCATC(5/9) SspBI 158 TGTACA T^GTACA TaqI 102 TCGA T^CGA TfiI 292 GATTC G^A[AT]TC Tru9I 229 TTAA T^TAA TseI 166 GCAGC G^C[AT]GC Tsp45I 254 GTCAC ^GT[GC]AC TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 102 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BstF5I 171 CATCC GGATG(2/0) 339 CATCC CfoI 143 GCGC GCG^C 164 GCGC Fnu4HI 151 GCCGC GC^[ACGT]GC 166 GCAGC FokI 171 CATCC GGATG(9/13) 339 CATCC HaeIII 36 GGCC GG^CC 91 GGCC HapII 29 CCGG C^CGG 71 CCGG HhaI 143 GCGC GCG^C 164 GCGC HinP1I 143 GCGC G^CGC 164 GCGC HinfI 25 GACTC G^A[ACGT]TC 292 GATTC HpaII 29 CCGG C^CGG 71 CCGG ItaI 151 GCCGC GC^[ACGT]GC 166 GCAGC MspI 29 CCGG C^CGG 71 CCGG Tsp509I 287 AATT ^AATT 312 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AciI AfaI BsrI Cac8I MboII MnlI MwoI RsaI