################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglII BlnI BpmI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DpnII DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MboI MfeI MflI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 823 ACATGT A^C[GA][CT]GT AlwNI 838 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 25 GAGCTC G[AT]GC[AT]^C BalI 557 TGGCCA TGG^CCA BciVI 215 GTATCC GTATCC(6/5) BglI 832 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 413 GCTCAGC GC^T[ACGT]AGC Bpu1102I 413 GCTCAGC GC^T[ACGT]AGC BsaBI 726 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 907 CTCCTC GAGGAG(10/8) BsiHKAI 25 GAGCTC G[AT]GC[AT]^C BsmAI 116 GAGAC GTCTC(1/5) BsmFI 484 GTCCC GGGAC(10/14) BspLU11I 823 ACATGT A^CATGT BstBI 64 TTCGAA TT^CGAA CelII 413 GCTCAGC GC^T[ACGT]AGC DraIII 886 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 557 TGGCCA [CT]^GGCC[GA] EcoRI 78 GAATTC G^AATTC FauI 935 CCCGC CCCGC(4/6) HincII 819 GTCAAC GT[CT]^[GA]AC HindII 819 GTCAAC GT[CT]^[GA]AC MamI 726 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 557 TGGCCA TGG^CCA MscI 557 TGGCCA TGG^CCA MslI 330 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 223 GCTAGC G^CTAGC NspI 823 ACATGT [GA]CATG^[CT] NspV 64 TTCGAA TT^CGAA PflMI 462 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 482 AGGTCCC [GA]G^G[AT]CC[CT] SacI 25 GAGCTC GAGCT^C SfcI 632 CTGTAG C^T[GA][CT]AG SfuI 64 TTCGAA TT^CGAA SmlI 48 CTCAAG C^T[CT][GA]AG StuI 489 AGGCCT AGG^CCT TaqI 65 TCGA T^CGA TfiI 100 GATTC G^A[AT]TC Tsp45I 550 GTCAC ^GT[GC]AC TthHB8I 65 TCGA T^CGA Van91I 462 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 78 GAATTC [GA]^AATT[CT] 515 GAATTT ApoI 78 GAATTC [GA]^AATT[CT] 515 GAATTT BanII 25 GAGCTC G[GA]GC[CT]^C 502 GGGCTC BspMI 164 ACCTGC ACCTGC(4/8) 848 ACCTGC BstXI 203 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 715 CCAGGCCCCTGG DdeI 41 CTGAG C^T[ACGT]AG 414 CTCAG DraII 482 AGGTCCC [GA]G^G[ACGT]CC[CT] 717 AGGCCCC EcoO109I 482 AGGTCCC [GA]G^G[ACGT]CC[CT] 717 AGGCCCC EcoT14I 683 CCAAGG C^C[AT][AT]GG 993 CCTTGG MaeIII 161 GTAAC ^GT[ACGT]AC 550 GTCAC PleI 175 GACTC GAGTC(4/5) 578 GACTC StyI 683 CCAAGG C^C[AT][AT]GG 993 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BfaI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI TseI Tsp509I TspRI