################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 944 ACGTGT A^C[GA][CT]GT AocI 841 CCTGAGG CC^T[ACGT]AGG ApaI 747 GGGCCC GGGCC^C AvaI 901 CCCGAG C^[CT]CG[GA]G BbeI 40 GGCGCC GGCGC^C BbsI 769 GTCTTC GAAGAC(2/6) BcgI 968 GCACGCGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 769 GTCTTC GAAGAC(2/6) BsaWI 257 ACCGGA [AT]^CCGG[AT] BseRI 904 GAGGAG GAGGAG(10/8) BsmBI 99 CGTCTC CGTCTC(1/5) BsoBI 901 CCCGAG C^[CT]CG[GA]G BspHI 107 TCATGA T^CATGA BsrFI 674 GCCGGC [GA]^CCGG[CT] BstEII 739 GGTCACC G^GT[ACGT]ACC BstPI 739 GGTCACC G^GT[ACGT]ACC BstXI 614 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 841 CCTGAGG CC^T[ACGT]AGG Cfr10I 674 GCCGGC [GA]^CCGG[CT] DraII 746 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 121 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 957 GAAGAG CTCTTC(1/4) Eco81I 841 CCTGAGG CC^T[ACGT]AGG EcoO109I 746 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 739 GGTCACC G^GT[ACGT]ACC KasI 40 GGCGCC G^GCGCC Ksp632I 957 GAAGAG CTCTTC(1/4) NaeI 674 GCCGGC GCC^GGC NarI 40 GGCGCC GG^CGCC NgoMIV 674 GCCGGC G^CCGGC PleI 295 GAGTC GAGTC(4/5) RcaI 107 TCATGA T^CATGA SapI 957 GAAGAGC GCTCTTC(1/4) ScaI 881 AGTACT AGT^ACT SfoI 40 GGCGCC GGC^GCC SmlI 592 CTCAAG C^T[CT][GA]AG StuI 845 AGGCCT AGG^CCT TfiI 319 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AspHI 285 GTGCTC G[AT]GC[AT]^C 517 GTGCTC BbrPI 325 CACGTG CAC^GTG 943 CACGTG BsaAI 325 CACGTG [CT]AC^GT[GA] 943 CACGTG BsgI 60 CTGCAC GTGCAG(16/14) 484 GTGCAG BsiEI 299 CGGCCG CG[GA][CT]^CG 479 CGGCCG BsiHKAI 285 GTGCTC G[AT]GC[AT]^C 517 GTGCTC BsmAI 247 GAGAC GTCTC(1/5) 100 GTCTC BssSI 679 CACGAG CACGAG(-5/-1) 1036 CACGAG BstF5I 789 CATCC GGATG(2/0) 858 CATCC BstYI 173 AGATCC [GA]^GATC[CT] 731 GGATCT EagI 299 CGGCCG C^GGCCG 479 CGGCCG EclXI 299 CGGCCG C^GGCCG 479 CGGCCG Eco52I 299 CGGCCG C^GGCCG 479 CGGCCG EcoT14I 46 CCAAGG C^C[AT][AT]GG 137 CCAAGG FokI 789 CATCC GGATG(9/13) 858 CATCC HinfI 295 GAGTC G^A[ACGT]TC 319 GAATC KspI 819 CCGCGG CCGC^GG 913 CCGCGG MaeIII 740 GTCAC ^GT[ACGT]AC 826 GTCAC MflI 173 AGATCC [GA]^GATC[CT] 731 GGATCT MslI 542 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1044 CATGAAAATG NspI 698 GCATGC [GA]CATG^[CT] 1011 GCATGC PflMI 324 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 857 CCATCCCCTGG PmaCI 325 CACGTG CAC^GTG 943 CACGTG PmlI 325 CACGTG CAC^GTG 943 CACGTG PvuII 754 CAGCTG CAG^CTG 1006 CAGCTG SacII 819 CCGCGG CCGC^GG 913 CCGCGG SphI 698 GCATGC GCATG^C 1011 GCATGC StyI 46 CCAAGG C^C[AT][AT]GG 137 CCAAGG TaqI 668 TCGA T^CGA 977 TCGA Tsp45I 740 GTCAC ^GT[GC]AC 826 GTCAC TspRI 924 CAGTG CA[GC]TG[ACGT][ACGT]^ 995 CACTG TthHB8I 668 TCGA T^CGA 977 TCGA Van91I 324 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 857 CCATCCCCTGG XcmI 788 CCATCCGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 989 CCAGGGCACTGCTGG XhoII 173 AGATCC [GA]^GATC[CT] 731 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI