(Linear) (Six-base) MAPSORT of: KIEE2166.seq Check: 1528 from: 1 to: 1059 KIEE2166.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 1059 Size: 236 823 AflIII A'CryG_T Cuts at: 0 944 1059 Size: 944 115 AleI CACnn'nnGTG Cuts at: 0 546 1059 Size: 546 513 ApaI G_GGCC'C Cuts at: 0 751 1059 Size: 751 308 AvaI C'yCGr_G Cuts at: 0 901 1059 Size: 901 158 BanI G'GyrC_C Cuts at: 0 40 377 625 1059 Size: 40 337 248 434 Fragments arranged by size: 434 337 248 40 BanII G_rGCy'C Cuts at: 0 137 503 751 1059 Size: 137 366 248 308 Fragments arranged by size: 366 308 248 137 BbeI G_GCGC'C Cuts at: 0 44 1059 Size: 44 1015 BbsI GAAGACnn'nnnn_ Cuts at: 0 762 1059 Size: 762 297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 957 991 1059 Size: 957 34 68 Fragments arranged by size: 957 68 34 Bme1580I G_kGCm'C Cuts at: 0 628 751 970 996 1059 Size: 628 123 219 26 63 Fragments arranged by size: 628 219 123 63 26 BmgBI CAC'GTC Cuts at: 0 986 1059 Size: 986 73 BmrI ACTGGGnnnn_n' Cuts at: 0 624 1059 Size: 624 435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 265 516 1059 Size: 209 56 251 543 Fragments arranged by size: 543 251 209 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 577 1059 Size: 577 482 BsaAI yAC'GTr Cuts at: 0 327 945 1059 Size: 327 618 114 Fragments arranged by size: 618 327 114 BsaHI Gr'CG_yC Cuts at: 0 41 527 824 1059 Size: 41 486 297 235 Fragments arranged by size: 486 297 235 41 BsaWI w'CCGG_w Cuts at: 0 257 1059 Size: 257 802 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 919 1059 Size: 919 140 BseYI C'CCAG_C Cuts at: 0 494 621 1004 1059 Size: 494 127 383 55 Fragments arranged by size: 494 383 127 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 505 1059 Size: 45 460 554 Fragments arranged by size: 554 460 45 BsiEI CG_ry'CG Cuts at: 0 302 482 1059 Size: 302 180 577 Fragments arranged by size: 577 302 180 BsiHKAI G_wGCw'C Cuts at: 0 289 521 1059 Size: 289 232 538 Fragments arranged by size: 538 289 232 BsmBI CGTCTCn'nnnn_ Cuts at: 0 105 1059 Size: 105 954 Bsp1286I G_dGCh'C Cuts at: 0 137 289 503 521 628 751 970 996 Size: 137 152 214 18 107 123 219 26 Cuts at: 996 1059 Size: 63 Fragments arranged by size: 219 214 152 137 123 107 63 26 18 BspHI T'CATG_A Cuts at: 0 107 1059 Size: 107 952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 170 443 515 608 1059 Size: 170 273 72 93 451 Fragments arranged by size: 451 273 170 93 72 BsrFI r'CCGG_y Cuts at: 0 674 1059 Size: 674 385 BssSI C'ACGA_G Cuts at: 0 679 1036 1059 Size: 679 357 23 Fragments arranged by size: 679 357 23 BstEII G'GTnAC_C Cuts at: 0 739 1059 Size: 739 320 BstXI CCAn_nnnn'nTGG Cuts at: 0 621 1059 Size: 621 438 BstYI r'GATC_y Cuts at: 0 173 731 1059 Size: 173 558 328 Fragments arranged by size: 558 328 173 Bsu36I CC'TnA_GG Cuts at: 0 842 1059 Size: 842 217 BtgI C'CryG_G Cuts at: 0 213 819 913 1059 Size: 213 606 94 146 Fragments arranged by size: 606 213 146 94 BtsI GCAGTG_nn' Cuts at: 0 994 1059 Size: 994 65 DraIII CAC_nnn'GTG Cuts at: 0 126 1059 Size: 126 933 EaeI y'GGCC_r Cuts at: 0 299 464 479 800 1059 Size: 299 165 15 321 259 Fragments arranged by size: 321 299 259 165 15 EagI C'GGCC_G Cuts at: 0 299 479 1059 Size: 299 180 580 Fragments arranged by size: 580 299 180 EarI CTCTTCn'nnn_ Cuts at: 0 952 1059 Size: 952 107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 236 265 516 1059 Size: 209 27 29 251 543 Fragments arranged by size: 543 251 209 29 27 EcoO109I rG'GnC_Cy Cuts at: 0 747 1059 Size: 747 312 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 900 1059 Size: 900 159 HaeII r_GCGC'y Cuts at: 0 44 495 675 694 1059 Size: 44 451 180 19 365 Fragments arranged by size: 451 365 180 44 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 723 755 970 1002 1059 Size: 723 32 215 32 57 Fragments arranged by size: 723 215 57 32 32 KasI G'GCGC_C Cuts at: 0 40 1059 Size: 40 1019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 548 1059 Size: 548 511 MslI CAynn'nnrTG Cuts at: 0 546 1048 1059 Size: 546 502 11 Fragments arranged by size: 546 502 11 MspA1I CmG'CkG Cuts at: 0 54 429 756 821 915 1008 1059 Size: 54 375 327 65 94 93 51 Fragments arranged by size: 375 327 94 93 65 54 51 NaeI GCC'GGC Cuts at: 0 676 1059 Size: 676 383 NarI GG'CG_CC Cuts at: 0 41 1059 Size: 41 1018 NgoMIV G'CCGG_C Cuts at: 0 674 1059 Size: 674 385 NspI r_CATG'y Cuts at: 0 702 1015 1059 Size: 702 313 44 Fragments arranged by size: 702 313 44 PflMI CCAn_nnn'nTGG Cuts at: 0 330 863 1059 Size: 330 533 196 Fragments arranged by size: 533 330 196 PfoI T'CCnGG_A Cuts at: 0 242 449 532 1059 Size: 242 207 83 527 Fragments arranged by size: 527 242 207 83 PmlI CAC'GTG Cuts at: 0 327 945 1059 Size: 327 618 114 Fragments arranged by size: 618 327 114 PspOMI G'GGCC_C Cuts at: 0 747 1059 Size: 747 312 PvuII CAG'CTG Cuts at: 0 756 1008 1059 Size: 756 252 51 Fragments arranged by size: 756 252 51 SacII CC_GC'GG Cuts at: 0 822 916 1059 Size: 822 94 143 Fragments arranged by size: 822 143 94 SapI GCTCTTCn'nnn_ Cuts at: 0 952 1059 Size: 952 107 ScaI AGT'ACT Cuts at: 0 883 1059 Size: 883 176 SfoI GGC'GCC Cuts at: 0 42 1059 Size: 42 1017 SmlI C'TyrA_G Cuts at: 0 592 1059 Size: 592 467 SphI G_CATG'C Cuts at: 0 702 1015 1059 Size: 702 313 44 Fragments arranged by size: 702 313 44 StuI AGG'CCT Cuts at: 0 847 1059 Size: 847 212 StyI C'CwwG_G Cuts at: 0 46 137 1059 Size: 46 91 922 Fragments arranged by size: 922 91 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 643 1059 Size: 643 416 TatI w'GTAC_w Cuts at: 0 881 1052 1059 Size: 881 171 7 Fragments arranged by size: 881 171 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 304 336 1059 Size: 304 32 723 Fragments arranged by size: 723 304 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 795 996 1059 Size: 795 201 63 Fragments arranged by size: 795 201 63 Enzymes that do cut: AcuI AflIII AleI ApaI AvaI BanI BanII BbeI BbsI BcgI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PmlI PspOMI PvuII SacII SapI ScaI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI BsaXI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI