(Circular) (Six-base) MAPSORT of: pFN21AB5292.seq Check: 1653 from: 1 to: 5941 pFN21AB5292 5941 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3168 3751 278 Size: 53 83 186 936 1632 583 2468 Fragments arranged by size: 2468 1632 936 583 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 2408 2451 3262 2408 Size: 43 811 5087 Fragments arranged by size: 5087 811 43 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 4004 5388 5656 1632 Size: 1412 960 1384 268 1917 Fragments arranged by size: 1917 1412 1384 960 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 2470 4670 2470 Size: 2200 3741 AleI CACnn'nnGTG Cuts at: 1166 2396 1166 Size: 1230 4711 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 2675 Size: 32 5909 AlwNI CAG_nnn'CTG Cuts at: 2504 2671 3684 5256 2504 Size: 167 1013 1572 3189 Fragments arranged by size: 3189 1572 1013 167 ApaI G_GGCC'C Cuts at: 2202 2915 2202 Size: 713 5228 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2207 2434 3266 1870 Size: 73 264 227 832 4545 Fragments arranged by size: 4545 832 264 227 73 BamHI G'GATC_C Cuts at: 2073 2358 3153 3271 3638 2073 Size: 285 795 118 367 4376 Fragments arranged by size: 4376 795 367 285 118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2408 2451 2476 3262 Size: 325 217 627 392 229 43 25 786 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 786 627 392 325 229 217 43 25 BanII G_rGCy'C Cuts at: 1391 2129 2202 2303 2915 3260 1391 Size: 738 73 101 612 345 4072 Fragments arranged by size: 4072 738 612 345 101 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2281 2638 3098 3160 928 Size: 971 382 357 460 62 3709 Fragments arranged by size: 3709 971 460 382 357 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4132 4166 1903 Size: 34 50 34 2111 34 3678 Fragments arranged by size: 3678 2111 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2951 3108 4552 Size: 107 122 71 631 605 1279 157 1444 Cuts at: 4552 136 Size: 1525 Fragments arranged by size: 1525 1444 1279 631 605 157 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2202 2481 2915 4002 5158 1790 Size: 412 279 434 1087 1156 2573 Fragments arranged by size: 2573 1156 1087 434 412 279 BmgBI CAC'GTC Cuts at: 1318 2094 1318 Size: 776 5165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2230 2266 3672 Size: 416 53 83 399 24 813 36 1406 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 1406 958 813 416 399 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 4601 1239 Size: 760 2602 2579 Fragments arranged by size: 2602 2579 760 Bpu10I CC'TnA_GC Cuts at: 2456 2702 3104 2456 Size: 246 402 5293 Fragments arranged by size: 5293 402 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 3056 4066 4931 5229 2201 Size: 855 1010 865 298 2913 Fragments arranged by size: 2913 1010 865 855 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2398 3732 493 Size: 711 1194 1334 2702 Fragments arranged by size: 2702 1334 1194 711 BsaBI GATnn'nnATC Cuts at: 2255 5935 2255 Size: 3680 2261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2264 3165 3748 Size: 53 83 186 936 699 32 901 583 Cuts at: 3748 4130 4765 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 901 699 635 583 382 186 83 53 32 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 2675 2705 1178 Size: 30 165 30 882 30 360 30 4414 Fragments arranged by size: 4414 882 360 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 1186 Size: 1107 4834 BseYI C'CCAG_C Cuts at: 2743 3028 3137 5144 5640 2743 Size: 285 109 2007 496 3044 Fragments arranged by size: 3044 2007 496 285 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2034 2066 1163 Size: 573 298 32 5038 Fragments arranged by size: 5038 573 298 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3391 4152 5180 1540 Size: 275 186 1177 213 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 213 186 BsiHKAI G_wGCw'C Cuts at: 2217 4002 4087 5158 2217 Size: 1785 85 1071 3000 Fragments arranged by size: 3000 1785 1071 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2164 2164 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2202 2217 2303 2481 2915 3260 Size: 399 339 73 15 86 178 434 345 Cuts at: 3260 4002 4087 5158 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 434 399 345 339 178 86 85 73 15 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2524 2806 3296 844 Size: 1680 282 490 3489 Fragments arranged by size: 3489 1680 490 282 BsrBI CCG'CTC Cuts at: 1394 1823 2029 3108 3828 1394 Size: 429 206 1079 720 3507 Fragments arranged by size: 3507 1079 720 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2073 2358 3153 3271 3638 4023 4040 5492 Size: 101 285 795 118 367 385 17 1452 Cuts at: 5492 5578 5590 5936 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 795 385 367 346 285 118 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5389 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 2560 487 Size: 683 56 1106 228 3868 Fragments arranged by size: 3868 1106 683 228 56 BtsI GCAGTG_nn' Cuts at: 2329 3457 4251 4271 2329 Size: 1128 794 20 3999 Fragments arranged by size: 3999 1128 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2841 3175 3388 Size: 54 1425 50 275 18 1011 334 213 Cuts at: 3388 4277 5906 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1011 889 334 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 3388 1537 Size: 275 1363 213 4090 Fragments arranged by size: 4090 1363 275 213 EarI CTCTTCn'nnn_ Cuts at: 1353 3871 1353 Size: 2518 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 4528 4902 5048 387 Size: 1064 138 501 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 501 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 3044 4004 4601 5388 5656 1239 Size: 393 367 1045 960 597 787 268 1524 Fragments arranged by size: 1524 1045 960 787 597 393 367 268 EcoNI CCTnn'n_nnAGG Cuts at: 2459 2828 2861 2459 Size: 369 33 5539 Fragments arranged by size: 5539 369 33 EcoO109I rG'GnC_Cy Cuts at: 2137 2153 2198 2317 2710 2137 Size: 16 45 119 393 5368 Fragments arranged by size: 5368 393 119 45 16 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 2721 4447 2721 Size: 1726 4215 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2456 2488 2984 Size: 32 135 32 713 32 139 32 496 Cuts at: 2984 3016 4582 4614 4656 4688 1373 Size: 32 1566 32 42 32 2626 Fragments arranged by size: 2626 1566 713 496 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2629 3285 3531 669 Size: 988 281 691 656 246 3079 Fragments arranged by size: 3079 988 691 656 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 2412 2455 3266 2412 Size: 43 811 5087 Fragments arranged by size: 5087 811 43 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2331 5055 5239 1305 Size: 789 237 2724 184 2007 Fragments arranged by size: 2724 2007 789 237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 10 Size: 54 1425 343 1011 3108 Fragments arranged by size: 3108 1425 1011 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3119 3899 4258 4417 518 Size: 648 1230 723 780 359 159 2042 Fragments arranged by size: 2042 1230 780 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2847 2962 3141 3357 4034 5182 5427 Size: 474 767 115 179 216 677 1148 245 Cuts at: 5427 1606 Size: 2120 Fragments arranged by size: 2120 1148 767 677 474 245 216 179 115 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2509 1348 Size: 1161 4780 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2313 1108 Size: 1205 4736 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2675 2707 4010 4042 5523 5555 2675 Size: 32 1303 32 1481 32 3061 Fragments arranged by size: 3061 1481 1303 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2317 2710 2137 Size: 180 393 5368 Fragments arranged by size: 5368 393 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2198 2911 2198 Size: 713 5228 PstI C_TGCA'G Cuts at: 830 2940 3293 830 Size: 2110 353 3478 Fragments arranged by size: 3478 2110 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2317 2710 2317 Size: 393 5548 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 2168 2240 4189 5901 2168 Size: 72 1949 1712 2208 Fragments arranged by size: 2208 1949 1712 72 SexAI A'CCwGG_T Cuts at: 2829 2829 Size: 5941 SfcI C'TryA_G Cuts at: 826 1045 2936 3289 4424 5105 5296 826 Size: 219 1891 353 1135 681 191 1471 Fragments arranged by size: 1891 1471 1135 681 353 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2951 2951 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 3071 4045 4946 5208 820 Size: 197 926 273 855 974 901 262 1553 Fragments arranged by size: 1553 974 926 901 855 273 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2807 1517 Size: 1290 4651 StyI C'CwwG_G Cuts at: 513 1065 3380 513 Size: 552 2315 3074 Fragments arranged by size: 3074 2315 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 2735 3151 3968 3985 4138 4323 Size: 1255 627 528 416 817 17 153 185 Cuts at: 4323 4768 325 Size: 445 1498 Fragments arranged by size: 1498 1255 817 627 528 445 416 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2238 2370 Size: 274 80 33 51 1443 189 72 132 Cuts at: 2370 2976 4187 5899 96 Size: 606 1211 1712 138 Fragments arranged by size: 1712 1443 1211 606 274 189 138 132 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3138 3170 5901 5933 3138 Size: 32 2731 32 3146 Fragments arranged by size: 3146 2731 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2110 1316 Size: 794 5147 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 2687 4070 2687 Size: 1383 4558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3166 3749 276 Size: 53 83 186 936 1632 583 2468 Fragments arranged by size: 2468 1632 936 583 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfoI SrfI SwaI XcmI