################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SfiI SgrAI SnaBI SpeI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1321 GGTACC G^GTACC AccI 400 GTAGAC GT^[AC][GT]AC AcyI 451 GGCGCC G[GA]^CG[CT]C AhdI 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1106 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1321 GGTACC G^GTACC AspEI 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1179 TGGCCA TGG^CCA BbeI 451 GGCGCC GGCGC^C BbvCI 262 GCTGAGG CCTCAGC(-5/-2) BfaI 136 CTAG C^TAG BglI 535 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1439 AGATCT A^GATCT BsaHI 451 GGCGCC G[GA]^CG[CT]C Bsp1407I 1412 TGTACA T^GTACA BspHI 1515 TCATGA T^CATGA BsrDI 73 GCAATG GCAATG(2/0) BsrGI 1412 TGTACA T^GTACA BssSI 1528 CACGAG CACGAG(-5/-1) BstBI 85 TTCGAA TT^CGAA BstF5I 425 GGATG GGATG(2/0) DsaI 482 CCATGG C^C[GA][CT]GG EaeI 1179 TGGCCA [CT]^GGCC[GA] Eam1105I 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 315 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 425 GGATG GGATG(9/13) Hin1I 451 GGCGCC G[GA]^CG[CT]C KasI 451 GGCGCC G^GCGCC KpnI 1321 GGTACC GGTAC^C MaeI 136 CTAG C^TAG MluNI 1179 TGGCCA TGG^CCA MscI 1179 TGGCCA TGG^CCA NarI 451 GGCGCC GG^CGCC NcoI 482 CCATGG C^CATGG NspV 85 TTCGAA TT^CGAA PshAI 751 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1515 TCATGA T^CATGA SacI 31 GAGCTC GAGCT^C SexAI 861 ACCAGGT A^CC[AT]GGT SfoI 451 GGCGCC GGC^GCC SfuI 85 TTCGAA TT^CGAA SmaI 1015 CCCGGG CCC^GGG SspBI 1412 TGTACA T^GTACA StuI 1035 AGGCCT AGG^CCT Tsp45I 839 GTGAC ^GT[GC]AC XcmI 536 CCAAGGGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1015 CCCGGG C^CCGGG XmaI 1015 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 215 CGCG CG^CG 370 CGCG AcsI 14 AAATTC [GA]^AATT[CT] 1356 AAATTC AlwI 159 GATCC GGATC(4/5) 1207 GGATC ApaI 927 GGGCCC GGGCC^C 1406 GGGCCC ApoI 14 AAATTC [GA]^AATT[CT] 1356 AAATTC AvaI 573 CTCGGG C^[CT]CG[GA]G 1015 CCCGGG BbsI 461 GTCTTC GAAGAC(2/6) 1372 GTCTTC BpuAI 461 GTCTTC GAAGAC(2/6) 1372 GTCTTC BseRI 111 GAGGAG GAGGAG(10/8) 1531 GAGGAG BsoBI 573 CTCGGG C^[CT]CG[GA]G 1015 CCCGGG BstAPI 339 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 384 GCACTTGCTGC BstUI 215 CGCG CG^CG 370 CGCG BstYI 1207 GGATCT [GA]^GATC[CT] 1439 AGATCT HaeII 175 GGCGCT [GA]GCGC^[CT] 451 GGCGCC MflI 1207 GGATCT [GA]^GATC[CT] 1439 AGATCT MslI 483 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 949 CACCCAGGTG MspA1I 170 CCGCTG C[AC]G^C[GT]G 394 CAGCGG MvnI 215 CGCG CG^CG 370 CGCG NspI 488 GCATGC [GA]CATG^[CT] 1382 GCATGC PleI 596 GAGTC GAGTC(4/5) 1020 GAGTC SbfI 345 CCTGCAGG CCTGCA^GG 1111 CCTGCAGG SmlI 19 CTCAAG C^T[CT][GA]AG 36 CTTGAG SphI 488 GCATGC GCATG^C 1382 GCATGC Sse8387I 345 CCTGCAGG CCTGCA^GG 1111 CCTGCAGG TaqI 86 TCGA T^CGA 331 TCGA TthHB8I 86 TCGA T^CGA 331 TCGA XhoII 1207 GGATCT [GA]^GATC[CT] 1439 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp509I TspRI