(Circular) (Six-base) MAPSORT of: pFN21AE1339.seq Check: 2179 from: 1 to: 6265 pFN21AE1339 6265 bp With 182 enzymes: SgfI * December 17, 2009 17:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2533 3110 2533 Size: 577 5688 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2406 3608 1937 Size: 469 1202 4594 Fragments arranged by size: 4594 1202 469 Acc65I G'GTAC_C Cuts at: 3326 3586 3326 Size: 260 6005 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3330 4328 5712 5980 1632 Size: 1698 998 1384 268 1917 Fragments arranged by size: 1917 1698 1384 998 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 2278 4994 2278 Size: 2716 3549 AleI CACnn'nnGTG Cuts at: 1166 2958 1166 Size: 1792 4473 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 3116 4008 5580 3116 Size: 892 1572 3801 Fragments arranged by size: 3801 1572 892 ApaI G_GGCC'C Cuts at: 2936 3415 2936 Size: 479 5786 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2019 3361 3574 3800 3952 6061 1493 Size: 526 1342 213 226 152 2109 1697 Fragments arranged by size: 2109 1697 1342 526 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2578 3020 3590 1870 Size: 73 635 442 570 4545 Fragments arranged by size: 4545 635 570 442 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3310 3343 3310 Size: 33 6232 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2456 2501 2663 2960 3326 Size: 325 217 627 669 45 162 297 366 Cuts at: 3326 3586 5041 618 Size: 260 1455 1842 Fragments arranged by size: 1842 1455 669 627 366 325 297 260 217 162 45 BanII G_rGCy'C Cuts at: 1391 2040 2830 2936 3020 3146 3172 3415 3584 Size: 649 790 106 84 126 26 243 169 Cuts at: 3584 1391 Size: 4072 Fragments arranged by size: 4072 790 649 243 169 126 106 84 26 BbeI G_GCGC'C Cuts at: 2460 2460 Size: 6265 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 3370 928 Size: 971 560 911 3823 Fragments arranged by size: 3823 971 911 560 BbvCI CC'TCA_GC Cuts at: 2268 2268 Size: 6265 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 4876 136 Size: 107 122 71 631 605 874 2330 1525 Fragments arranged by size: 2330 1525 874 631 605 122 107 71 BglII A'GATC_T Cuts at: 3444 6260 3444 Size: 2816 3449 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2506 2936 3415 3476 4326 5482 1790 Size: 716 430 479 61 850 1156 2573 Fragments arranged by size: 2573 1156 850 716 479 430 61 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 3996 4954 Size: 416 53 83 399 24 2030 549 958 Cuts at: 4954 442 Size: 1753 Fragments arranged by size: 2030 1753 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2331 2625 2844 2907 4925 1239 Size: 1016 76 294 219 63 2018 2579 Fragments arranged by size: 2579 2018 1016 294 219 76 63 Bpu10I CC'TnA_GC Cuts at: 2268 2325 2268 Size: 57 6208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2009 2062 4390 5255 5553 2009 Size: 53 2328 865 298 2721 Fragments arranged by size: 2721 2328 865 298 53 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2714 2748 2768 3189 4928 882 Size: 617 543 672 34 20 421 1739 2219 Fragments arranged by size: 2219 1739 672 617 543 421 34 20 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2457 4072 4454 5089 Size: 53 83 186 936 924 1615 382 635 Cuts at: 5089 275 Size: 1451 Fragments arranged by size: 1615 1451 936 924 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2827 2857 1178 Size: 30 165 30 1424 30 4586 Fragments arranged by size: 4586 1424 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 3551 1186 Size: 945 1420 3900 Fragments arranged by size: 3900 1420 945 BseYI C'CCAG_C Cuts at: 2183 3391 3510 5468 5964 2183 Size: 1208 119 1958 496 2484 Fragments arranged by size: 2484 1958 1208 496 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2964 3481 3577 1163 Size: 573 1228 517 96 3851 Fragments arranged by size: 3851 1228 573 517 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2391 2641 4326 4411 5482 2040 Size: 351 250 1685 85 1071 2823 Fragments arranged by size: 2823 1685 1071 351 250 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2391 2506 2641 2830 2936 3020 Size: 399 250 351 115 135 189 106 84 Cuts at: 3020 3146 3172 3415 3476 3584 4326 4411 5482 Size: 126 26 243 61 108 742 85 1071 Cuts at: 5482 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 351 250 243 189 135 126 115 108 106 85 84 61 26 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 3520 4154 5884 3520 Size: 634 1730 3901 Fragments arranged by size: 3901 1730 634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2533 3110 3146 3620 844 Size: 1689 577 36 474 3489 Fragments arranged by size: 3489 1689 577 474 36 BsrBI CCG'CTC Cuts at: 1394 1823 4152 1394 Size: 429 2329 3507 Fragments arranged by size: 3507 2329 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2085 4760 4934 65 Size: 1183 837 2675 174 1396 Fragments arranged by size: 2675 1396 1183 837 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3417 96 Size: 3321 2944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 3533 4325 5337 3533 Size: 792 1012 4461 Fragments arranged by size: 4461 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 2350 2395 794 Size: 1556 45 4664 Fragments arranged by size: 4664 1556 45 BstBI TT'CG_AA Cuts at: 2091 5069 2091 Size: 2978 3287 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstYI r'GATC_y Cuts at: 1972 3212 3444 3595 3962 4347 4364 5816 5902 Size: 1240 232 151 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1240 385 367 346 232 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2487 513 Size: 552 1422 4291 Fragments arranged by size: 4291 1422 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2715 3360 487 Size: 683 56 1489 645 3392 Fragments arranged by size: 3392 1489 683 645 56 BtsI GCAGTG_nn' Cuts at: 2952 2986 3781 4575 4595 2952 Size: 34 795 794 20 4622 Fragments arranged by size: 4622 795 794 34 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2853 2888 529 Size: 35 2289 35 3906 Fragments arranged by size: 3906 2289 35 35 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3184 3712 4601 Size: 54 1425 50 275 18 1354 528 889 Cuts at: 4601 6230 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1354 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4195 1353 Size: 2842 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4852 5226 5372 387 Size: 1064 138 3263 374 146 1280 Fragments arranged by size: 3263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2038 2038 Size: 6265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2331 2625 2844 2907 3330 4328 Size: 393 623 76 294 219 63 423 998 Cuts at: 4328 4925 5712 5980 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 998 787 623 597 423 393 294 268 219 76 63 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 6265 EcoO109I rG'GnC_Cy Cuts at: 2230 2573 2582 2627 2672 2816 2933 3113 3158 Size: 343 9 45 45 144 117 180 45 Cuts at: 3158 3411 3412 2230 Size: 253 1 5083 Fragments arranged by size: 5083 343 253 180 144 117 45 45 45 9 1 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2184 2460 5412 1678 Size: 506 276 2952 2531 Fragments arranged by size: 2952 2531 506 276 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 4906 4938 4980 Size: 32 135 32 692 32 2610 32 42 Cuts at: 4980 5012 1373 Size: 32 2626 Fragments arranged by size: 2626 2610 692 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KasI G'GCGC_C Cuts at: 2456 2456 Size: 6265 KpnI G_GTAC'C Cuts at: 3330 3590 3330 Size: 260 6005 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5379 5563 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3186 10 Size: 54 1425 343 1354 3089 Fragments arranged by size: 3089 1425 1354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2958 4223 4582 4741 518 Size: 648 1326 466 1265 359 159 2042 Fragments arranged by size: 2042 1326 1265 648 466 359 159 MspA1I CmG'CkG Cuts at: 1606 2177 2401 3681 4358 5506 5751 1606 Size: 571 224 1280 677 1148 245 2120 Fragments arranged by size: 2120 1280 1148 677 571 245 224 NarI GG'CG_CC Cuts at: 2457 2457 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2487 513 Size: 552 1422 4291 Fragments arranged by size: 4291 1422 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 2497 3391 3623 5168 1132 Size: 97 1268 894 232 1545 2229 Fragments arranged by size: 2229 1545 1268 894 232 97 PasI CC'CwG_GG Cuts at: 1348 2426 2632 2830 2993 3146 1348 Size: 1078 206 198 163 153 4467 Fragments arranged by size: 4467 1078 206 198 163 153 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PfoI T'CCnGG_A Cuts at: 2232 2821 2884 3028 2232 Size: 589 63 144 5469 Fragments arranged by size: 5469 589 144 63 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2230 2582 2627 2672 3158 2230 Size: 352 45 45 486 5337 Fragments arranged by size: 5337 486 352 45 45 PshAI GACnn'nnGTC Cuts at: 1613 2760 1613 Size: 1147 5118 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 2932 3411 2932 Size: 479 5786 PstI C_TGCA'G Cuts at: 830 2217 2298 2355 2400 2922 3121 3530 3617 Size: 1387 81 57 45 522 199 409 87 Cuts at: 3617 830 Size: 3478 Fragments arranged by size: 3478 1387 522 409 199 87 81 57 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 SacI G_AGCT'C Cuts at: 2040 2040 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SanDI GG'GwC_CC Cuts at: 2582 2582 Size: 6265 SbfI CC_TGCA'GG Cuts at: 2355 3121 3617 2355 Size: 766 496 5003 Fragments arranged by size: 5003 766 496 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SexAI A'CCwGG_T Cuts at: 2866 2866 Size: 6265 SfcI C'TryA_G Cuts at: 826 1045 2213 2294 2351 2396 2918 3117 3319 Size: 219 1168 81 57 45 522 199 202 Cuts at: 3319 3526 3613 4748 5429 5620 826 Size: 207 87 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 681 522 219 207 202 199 191 87 81 57 45 SfoI GGC'GCC Cuts at: 2458 2458 Size: 6265 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3022 3592 1872 Size: 1150 570 4545 Fragments arranged by size: 4545 1150 570 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 2041 4369 5270 5532 820 Size: 197 926 81 17 2328 901 262 1553 Fragments arranged by size: 2328 1553 926 901 262 197 81 17 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 2497 3391 3623 1132 Size: 1365 894 232 3774 Fragments arranged by size: 3774 1365 894 232 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 3042 1517 Size: 1525 4740 StyI C'CwwG_G Cuts at: 513 1065 2487 2541 2587 2623 2677 2964 3162 Size: 552 1422 54 46 36 54 287 198 Cuts at: 3162 3704 513 Size: 542 3074 Fragments arranged by size: 3074 1422 552 542 287 198 54 54 46 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2970 4292 4309 4462 4647 5092 325 Size: 1255 1390 1322 17 153 185 445 1498 Fragments arranged by size: 1498 1390 1322 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3417 4511 6223 Size: 274 80 33 51 1443 1440 1094 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1712 1443 1440 1094 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2827 2859 6225 6257 2827 Size: 32 3366 32 2835 Fragments arranged by size: 3366 2835 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2548 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 3020 3590 1870 Size: 1150 570 4545 Fragments arranged by size: 4545 1150 570 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI