################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BcgI BciVI BfrI BglII BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 1457 GACGTC GACGT^C AccII 535 CGCG CG^CG Alw44I 896 GTGCAC G^TGCAC ApaLI 896 GTGCAC G^TGCAC AspI 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1940 TGGCCA TGG^CCA BbrPI 58 CACGTG CAC^GTG BbsI 1663 GAAGAC GAAGAC(2/6) BbvCI 1163 CCTCAGC CCTCAGC(-5/-2) BclI 113 TGATCA T^GATCA BglI 1933 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1663 GAAGAC GAAGAC(2/6) BsaAI 58 CACGTG [CT]AC^GT[GA] BsaBI 1386 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BssSI 1249 CTCGTG CACGAG(-5/-1) BstUI 535 CGCG CG^CG DrdI 1451 GACATCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 113 TGATCA T^GATCA HgaI 1101 GCGTC GACGC(5/10) MamI 1386 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1940 TGGCCA TGG^CCA MscI 1940 TGGCCA TGG^CCA MvnI 535 CGCG CG^CG NspI 273 ACATGC [GA]CATG^[CT] PflFI 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 58 CACGTG CAC^GTG PmlI 58 CACGTG CAC^GTG SbfI 1510 CCTGCAGG CCTGCA^GG Sse8387I 1510 CCTGCAGG CCTGCA^GG StuI 1545 AGGCCT AGG^CCT Tth111I 970 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 1141 GAATTC [GA]^AATT[CT] 1750 GAATTC AcyI 1100 GGCGTC G[GA]^CG[CT]C 1457 GACGTC AlwNI 104 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 1514 CAGGGGCTG ApoI 1141 GAATTC [GA]^AATT[CT] 1750 GAATTC AvaI 31 CTCGGG C^[CT]CG[GA]G 564 CCCGAG AvrII 657 CCTAGG C^CTAGG 1708 CCTAGG BamHI 215 GGATCC G^GATCC 1347 GGATCC BanI 71 GGCACC G^G[CT][GA]CC 1370 GGCACC BfaI 658 CTAG C^TAG 1709 CTAG BlnI 657 CCTAGG C^CTAGG 1708 CCTAGG BsaHI 1100 GGCGTC G[GA]^CG[CT]C 1457 GACGTC BsmFI 1177 GGGAC GGGAC(10/14) 531 GTCCC BsoBI 31 CTCGGG C^[CT]CG[GA]G 564 CCCGAG BspMI 1217 ACCTGC ACCTGC(4/8) 572 GCAGGT BsrBI 318 CCGCTC CCGCTC(-3/-3) 864 CCGCTC BstXI 392 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 516 CCATGCCCGTGG BstYI 215 GGATCC [GA]^GATC[CT] 1347 GGATCC CfoI 536 GCGC GCG^C 809 GCGC EaeI 1400 TGGCCG [CT]^GGCC[GA] 1940 TGGCCA EarI 1435 CTCTTC CTCTTC(1/4) 1780 GAAGAG EcoRI 1141 GAATTC G^AATTC 1750 GAATTC FauI 533 CCCGC CCCGC(4/6) 1174 GCGGG HhaI 536 GCGC GCG^C 809 GCGC Hin1I 1100 GGCGTC G[GA]^CG[CT]C 1457 GACGTC HinP1I 536 GCGC G^CGC 809 GCGC Ksp632I 1435 CTCTTC CTCTTC(1/4) 1780 GAAGAG MaeI 658 CTAG C^TAG 1709 CTAG MflI 215 GGATCC [GA]^GATC[CT] 1347 GGATCC NcoI 1096 CCATGG C^CATGG 1148 CCATGG Tsp509I 1142 AATT ^AATT 1751 AATT XhoII 215 GGATCC [GA]^GATC[CT] 1347 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwI AocI ApaI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII DsaI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI