(Linear) (Six-base) MAPSORT of: KIEE1454.seq Check: 499 from: 1 to: 1950 KIEE1454.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 563 1950 Size: 563 1387 AatII G_ACGT'C Cuts at: 0 1461 1950 Size: 1461 489 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 1838 1950 Size: 781 1057 112 Fragments arranged by size: 1057 781 112 AleI CACnn'nnGTG Cuts at: 0 1232 1950 Size: 1232 718 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 776 808 1950 Size: 776 32 1142 Fragments arranged by size: 1142 776 32 AlwNI CAG_nnn'CTG Cuts at: 0 109 1519 1950 Size: 109 1410 431 Fragments arranged by size: 1410 431 109 ApaI G_GGCC'C Cuts at: 0 49 556 856 1000 1276 1907 1950 Size: 49 507 300 144 276 631 43 Fragments arranged by size: 631 507 300 276 144 49 43 ApaLI G'TGCA_C Cuts at: 0 896 1950 Size: 896 1054 ApoI r'AATT_y Cuts at: 0 1141 1750 1950 Size: 1141 609 200 Fragments arranged by size: 1141 609 200 AvaI C'yCGr_G Cuts at: 0 31 564 1950 Size: 31 533 1386 Fragments arranged by size: 1386 533 31 AvrII C'CTAG_G Cuts at: 0 657 1708 1950 Size: 657 1051 242 Fragments arranged by size: 1051 657 242 BamHI G'GATC_C Cuts at: 0 215 1347 1950 Size: 215 1132 603 Fragments arranged by size: 1132 603 215 BanI G'GyrC_C Cuts at: 0 71 1370 1950 Size: 71 1299 580 Fragments arranged by size: 1299 580 71 BanII G_rGCy'C Cuts at: 0 49 556 565 650 830 856 1000 1207 Size: 49 507 9 85 180 26 144 207 Cuts at: 1207 1250 1276 1535 1654 1907 1950 Size: 43 26 259 119 253 43 Fragments arranged by size: 507 259 253 207 180 144 119 85 49 43 43 26 26 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 1670 1950 Size: 1670 280 BbvCI CC'TCA_GC Cuts at: 0 1164 1950 Size: 1164 786 BclI T'GATC_A Cuts at: 0 113 1950 Size: 113 1837 BglI GCCn_nnn'nGGC Cuts at: 0 1939 1950 Size: 1939 11 Bme1580I G_kGCm'C Cuts at: 0 49 74 556 856 900 1000 1276 1907 Size: 49 25 482 300 44 100 276 631 Cuts at: 1907 1922 1950 Size: 15 28 Fragments arranged by size: 631 482 300 276 100 49 44 28 25 15 BmrI ACTGGGnnnn_n' Cuts at: 0 626 1226 1950 Size: 626 600 724 Fragments arranged by size: 724 626 600 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 997 1950 Size: 997 953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 412 664 1264 1686 1895 1950 Size: 361 51 252 600 422 209 55 Fragments arranged by size: 600 422 361 252 209 55 51 Bpu10I CC'TnA_GC Cuts at: 0 1164 1950 Size: 1164 786 BsaI GGTCTCn'nnnn_ Cuts at: 0 154 629 712 1260 1950 Size: 154 475 83 548 690 Fragments arranged by size: 690 548 475 154 83 BsaAI yAC'GTr Cuts at: 0 60 1950 Size: 60 1890 BsaBI GATnn'nnATC Cuts at: 0 1390 1950 Size: 1390 560 BsaHI Gr'CG_yC Cuts at: 0 1101 1458 1950 Size: 1101 357 492 Fragments arranged by size: 1101 492 357 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 404 434 892 895 922 925 961 991 Size: 404 30 458 3 27 3 36 30 Cuts at: 991 1007 1037 1403 1433 1869 1899 1950 Size: 16 30 366 30 436 30 51 Fragments arranged by size: 458 436 404 366 51 36 30 30 30 30 30 27 16 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 730 900 1408 1411 1414 1417 1420 1423 Size: 730 170 508 3 3 3 3 3 Cuts at: 1423 1718 1877 1950 Size: 295 159 73 Fragments arranged by size: 730 508 295 170 159 73 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 386 391 509 823 928 1276 1566 1844 Size: 386 5 118 314 105 348 290 278 Cuts at: 1844 1921 1950 Size: 77 29 Fragments arranged by size: 386 348 314 290 278 118 105 77 29 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 709 1009 1834 1950 Size: 82 627 300 825 116 Fragments arranged by size: 825 627 300 116 82 BsiHKAI G_wGCw'C Cuts at: 0 650 900 1207 1250 1950 Size: 650 250 307 43 700 Fragments arranged by size: 700 650 307 250 43 Bsp1286I G_dGCh'C Cuts at: 0 49 74 556 565 650 830 856 900 Size: 49 25 482 9 85 180 26 44 Cuts at: 900 1000 1207 1250 1276 1535 1654 1907 1922 Size: 100 207 43 26 259 119 253 15 Cuts at: 1922 1950 Size: 28 Fragments arranged by size: 482 259 253 207 180 119 100 85 49 44 43 28 26 26 25 15 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 563 1226 1950 Size: 563 663 724 Fragments arranged by size: 724 663 563 BsrBI CCG'CTC Cuts at: 0 320 866 1950 Size: 320 546 1084 Fragments arranged by size: 1084 546 320 BssSI C'ACGA_G Cuts at: 0 1249 1950 Size: 1249 701 BstXI CCAn_nnnn'nTGG Cuts at: 0 399 523 1950 Size: 399 124 1427 Fragments arranged by size: 1427 399 124 BstYI r'GATC_y Cuts at: 0 215 1347 1950 Size: 215 1132 603 Fragments arranged by size: 1132 603 215 Bsu36I CC'TnA_GG Cuts at: 0 139 713 1011 1316 1622 1950 Size: 139 574 298 305 306 328 Fragments arranged by size: 574 328 306 305 298 139 BtgI C'CryG_G Cuts at: 0 398 522 548 1001 1096 1148 1950 Size: 398 124 26 453 95 52 802 Fragments arranged by size: 802 453 398 124 95 52 26 BtsI GCAGTG_nn' Cuts at: 0 308 336 739 1558 1682 1950 Size: 308 28 403 819 124 268 Fragments arranged by size: 819 403 308 268 124 28 DrdI GACnn_nn'nnGTC Cuts at: 0 1457 1950 Size: 1457 493 EaeI y'GGCC_r Cuts at: 0 1400 1940 1950 Size: 1400 540 10 Fragments arranged by size: 1400 540 10 EarI CTCTTCn'nnn_ Cuts at: 0 1441 1775 1950 Size: 1441 334 175 Fragments arranged by size: 1441 334 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1792 1950 Size: 1792 158 EcoICRI GAG'CTC Cuts at: 0 648 1205 1248 1950 Size: 648 557 43 702 Fragments arranged by size: 702 648 557 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 412 664 781 1264 1686 1838 1895 Size: 361 51 252 117 483 422 152 57 Cuts at: 1895 1950 Size: 55 Fragments arranged by size: 483 422 361 252 152 117 57 55 51 EcoO109I rG'GnC_Cy Cuts at: 0 46 553 697 853 997 1153 1178 1272 Size: 46 507 144 156 144 156 25 94 Cuts at: 1272 1273 1294 1903 1904 1950 Size: 1 21 609 1 46 Fragments arranged by size: 609 507 156 156 144 144 94 46 46 25 21 1 1 EcoRI G'AATT_C Cuts at: 0 1141 1750 1950 Size: 1141 609 200 Fragments arranged by size: 1141 609 200 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 350 382 552 584 592 624 711 743 Size: 350 32 170 32 8 32 87 32 Cuts at: 743 961 993 1629 1661 1950 Size: 218 32 636 32 289 Fragments arranged by size: 636 350 289 218 170 87 32 32 32 32 32 32 8 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1325 1473 1527 1950 Size: 1325 148 54 423 Fragments arranged by size: 1325 423 148 54 MscI TGG'CCA Cuts at: 0 1942 1950 Size: 1942 8 MslI CAynn'nnrTG Cuts at: 0 521 1047 1232 1396 1950 Size: 521 526 185 164 554 Fragments arranged by size: 554 526 521 185 164 MspA1I CmG'CkG Cuts at: 0 1087 1570 1848 1915 1950 Size: 1087 483 278 67 35 Fragments arranged by size: 1087 483 278 67 35 NcoI C'CATG_G Cuts at: 0 1096 1148 1950 Size: 1096 52 802 Fragments arranged by size: 1096 802 52 NspI r_CATG'y Cuts at: 0 277 1950 Size: 277 1673 PasI CC'CwG_GG Cuts at: 0 120 849 1950 Size: 120 729 1101 Fragments arranged by size: 1101 729 120 PflMI CCAn_nnn'nTGG Cuts at: 0 92 266 1744 1950 Size: 92 174 1478 206 Fragments arranged by size: 1478 206 174 92 PfoI T'CCnGG_A Cuts at: 0 641 1754 1950 Size: 641 1113 196 Fragments arranged by size: 1113 641 196 PmlI CAC'GTG Cuts at: 0 60 1950 Size: 60 1890 PpuMI rG'GwC_Cy Cuts at: 0 697 1153 1178 1950 Size: 697 456 25 772 Fragments arranged by size: 772 697 456 25 PspOMI G'GGCC_C Cuts at: 0 45 552 852 996 1272 1903 1950 Size: 45 507 300 144 276 631 47 Fragments arranged by size: 631 507 300 276 144 47 45 PstI C_TGCA'G Cuts at: 0 315 734 1515 1553 1950 Size: 315 419 781 38 397 Fragments arranged by size: 781 419 397 315 38 PvuII CAG'CTG Cuts at: 0 1087 1570 1848 1915 1950 Size: 1087 483 278 67 35 Fragments arranged by size: 1087 483 278 67 35 SacI G_AGCT'C Cuts at: 0 650 1207 1250 1950 Size: 650 557 43 700 Fragments arranged by size: 700 650 557 43 SbfI CC_TGCA'GG Cuts at: 0 1515 1950 Size: 1515 435 SfcI C'TryA_G Cuts at: 0 311 730 1182 1511 1549 1950 Size: 311 419 452 329 38 401 Fragments arranged by size: 452 419 401 329 311 38 StuI AGG'CCT Cuts at: 0 1547 1950 Size: 1547 403 StyI C'CwwG_G Cuts at: 0 657 1073 1096 1148 1708 1950 Size: 657 416 23 52 560 242 Fragments arranged by size: 657 560 416 242 52 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 133 506 1950 Size: 133 373 1444 Fragments arranged by size: 1444 373 133 TatI w'GTAC_w Cuts at: 0 881 1950 Size: 881 1069 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 892 924 1007 1039 1950 Size: 892 32 83 32 911 Fragments arranged by size: 911 892 83 32 32 Tth111I GACn'n_nGTC Cuts at: 0 973 1950 Size: 973 977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 263 546 639 846 936 1239 1950 Size: 263 283 93 207 90 303 711 Fragments arranged by size: 711 303 283 263 207 93 90 ZraI GAC'GTC Cuts at: 0 1459 1950 Size: 1459 491 Enzymes that do cut: AarI AatII AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SbfI SfcI StuI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI BaeI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI