################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbsI BcgI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MluI MroI MslI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AccII 113 CGCG CG^CG AciI 363 CCGC CCGC(-3/-1) AfaI 131 GTAC GT^AC AhdI 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 18 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 323 GGACC G^G[AT]CC BalI 35 TGGCCA TGG^CCA BamHI 243 GGATCC G^GATCC BbvCI 157 GCTGAGG CCTCAGC(-5/-2) BbvI 23 GCTGC GCAGC(8/12) BciVI 359 GTATCC GTATCC(6/5) BcnI 13 CCCGG CC^[GC]GG BglI 365 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 92 GCTCAGC GC^T[ACGT]AGC BmyI 29 GTGCCC G[AGT]GC[ACT]^C BpmI 194 CTGGAG CTGGAG(16/14) Bpu1102I 92 GCTCAGC GC^T[ACGT]AGC BsaI 211 GAGACC GGTCTC(1/5) BsmFI 322 GGGAC GGGAC(10/14) Bsp1286I 29 GTGCCC G[AGT]GC[ACT]^C BstAPI 17 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 513 GGTCACC G^GT[ACGT]ACC BstPI 513 GGTCACC G^GT[ACGT]ACC BstUI 113 CGCG CG^CG BstYI 243 GGATCC [GA]^GATC[CT] Cac8I 45 GCTGGC GC[ACGT]^[ACGT]GC CelII 92 GCTCAGC GC^T[ACGT]AGC DpnII 244 GATC ^GATC DraI 219 TTTAAA TTT^AAA DraII 322 GGGACCT [GA]G^G[ACGT]CC[CT] EaeI 35 TGGCCA [CT]^GGCC[GA] Eam1105I 58 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 151 GAAGAG CTCTTC(1/4) EcoO109I 322 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 513 GGTCACC G^GT[ACGT]ACC EcoT22I 75 ATGCAT ATGCA^T Fnu4HI 23 GCTGC GC^[ACGT]GC HapII 14 CCGG C^CGG HindIII 536 AAGCTT A^AGCTT HpaII 14 CCGG C^CGG ItaI 23 GCTGC GC^[ACGT]GC Ksp632I 151 GAAGAG CTCTTC(1/4) MboI 244 GATC ^GATC MboII 151 GAAGA GAAGA(8/7) MfeI 50 CAATTG C^AATTG MflI 243 GGATCC [GA]^GATC[CT] MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MspA1I 226 CAGCTG C[AC]G^C[GT]G MspI 14 CCGG C^CGG MunI 50 CAATTG C^AATTG MvnI 113 CGCG CG^CG NciI 13 CCCGG CC^[GC]GG NdeII 244 GATC ^GATC NsiI 75 ATGCAT ATGCA^T PpuMI 322 GGGACCT [GA]G^G[AT]CC[CT] PvuII 226 CAGCTG CAG^CTG RsaI 131 GTAC GT^AC Sau3AI 244 GATC ^GATC SfaNI 74 GATGC GCATC(5/9) SmlI 445 CTTGAG C^T[CT][GA]AG TseI 23 GCTGC G^C[AT]GC Tsp509I 51 AATT ^AATT XhoII 243 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### AlwI 244 GATCC GGATC(4/5) 243 GGATC BsiYI 13 CCCGGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCCTTCAGG BslI 13 CCCGGCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 363 CCGCCTTCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BsaJI BsmAI BsrI BssKI BstF5I BstNI BstXI Cfr13I DdeI EcoRII FokI HaeIII HphI MaeIII MnlI MseI MvaI MwoI NlaIV Sau96I ScrFI Tru9I Tsp45I TspRI