(Linear) (Six-base) MAPSORT of: KIEE6237.seq Check: 6212 from: 1 to: 720 KIEE6237.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. Acc65I G'GTAC_C Cuts at: 0 473 720 Size: 473 247 AflIII A'CryG_T Cuts at: 0 110 720 Size: 110 610 AlwNI CAG_nnn'CTG Cuts at: 0 467 720 Size: 467 253 ApaI G_GGCC'C Cuts at: 0 389 508 644 720 Size: 389 119 136 76 Fragments arranged by size: 389 136 119 76 ApaLI G'TGCA_C Cuts at: 0 343 720 Size: 343 377 AvaI C'yCGr_G Cuts at: 0 227 426 643 720 Size: 227 199 217 77 Fragments arranged by size: 227 217 199 77 BamHI G'GATC_C Cuts at: 0 101 720 Size: 101 619 BanI G'GyrC_C Cuts at: 0 40 473 720 Size: 40 433 247 Fragments arranged by size: 433 247 40 BanII G_rGCy'C Cuts at: 0 80 389 508 644 650 720 Size: 80 309 119 136 6 70 Fragments arranged by size: 309 136 119 80 70 6 BglI GCCn_nnn'nGGC Cuts at: 0 487 569 720 Size: 487 82 151 Fragments arranged by size: 487 151 82 BglII A'GATC_T Cuts at: 0 131 720 Size: 131 589 Bme1580I G_kGCm'C Cuts at: 0 347 389 508 644 720 Size: 347 42 119 136 76 Fragments arranged by size: 347 136 119 76 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 99 720 Size: 99 621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 309 316 489 720 Size: 309 7 173 231 Fragments arranged by size: 309 231 173 7 Bpu10I CC'TnA_GC Cuts at: 0 361 720 Size: 361 359 BsaAI yAC'GTr Cuts at: 0 342 720 Size: 342 378 BsaHI Gr'CG_yC Cuts at: 0 50 215 239 281 720 Size: 50 165 24 42 439 Fragments arranged by size: 439 165 50 42 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 720 Size: 214 506 BsiEI CG_ry'CG Cuts at: 0 311 720 Size: 311 409 BsiHKAI G_wGCw'C Cuts at: 0 347 674 720 Size: 347 327 46 Fragments arranged by size: 347 327 46 Bsp1286I G_dGCh'C Cuts at: 0 80 347 389 508 644 650 674 720 Size: 80 267 42 119 136 6 24 46 Fragments arranged by size: 267 136 119 80 46 42 24 6 BsrFI r'CCGG_y Cuts at: 0 37 235 481 605 720 Size: 37 198 246 124 115 Fragments arranged by size: 246 198 124 115 37 BstAPI GCAn_nnn'nTGC Cuts at: 0 40 720 Size: 40 680 BstEII G'GTnAC_C Cuts at: 0 231 720 Size: 231 489 BstYI r'GATC_y Cuts at: 0 101 131 720 Size: 101 30 589 Fragments arranged by size: 589 101 30 BtgI C'CryG_G Cuts at: 0 530 564 720 Size: 530 34 156 Fragments arranged by size: 530 156 34 EaeI y'GGCC_r Cuts at: 0 203 404 488 720 Size: 203 201 84 232 Fragments arranged by size: 232 203 201 84 EarI CTCTTCn'nnn_ Cuts at: 0 253 720 Size: 253 467 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 88 109 720 Size: 88 21 611 Fragments arranged by size: 611 88 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 309 316 489 720 Size: 309 7 173 231 Fragments arranged by size: 309 231 173 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 364 720 Size: 364 356 EcoO109I rG'GnC_Cy Cuts at: 0 357 386 720 Size: 357 29 334 Fragments arranged by size: 357 334 29 HaeII r_GCGC'y Cuts at: 0 10 660 720 Size: 10 650 60 Fragments arranged by size: 650 60 10 KpnI G_GTAC'C Cuts at: 0 477 720 Size: 477 243 MslI CAynn'nnrTG Cuts at: 0 340 720 Size: 340 380 MspA1I CmG'CkG Cuts at: 0 153 402 566 720 Size: 153 249 164 154 Fragments arranged by size: 249 164 154 153 NaeI GCC'GGC Cuts at: 0 483 607 720 Size: 483 124 113 Fragments arranged by size: 483 124 113 NgoMIV G'CCGG_C Cuts at: 0 481 605 720 Size: 481 124 115 Fragments arranged by size: 481 124 115 NspI r_CATG'y Cuts at: 0 114 720 Size: 114 606 PciI A'CATG_T Cuts at: 0 110 720 Size: 110 610 PmlI CAC'GTG Cuts at: 0 342 720 Size: 342 378 PpuMI rG'GwC_Cy Cuts at: 0 357 720 Size: 357 363 PshAI GACnn'nnGTC Cuts at: 0 238 720 Size: 238 482 PspOMI G'GGCC_C Cuts at: 0 385 504 640 720 Size: 385 119 136 80 Fragments arranged by size: 385 136 119 80 RsrII CG'GwC_CG Cuts at: 0 450 720 Size: 450 270 SacII CC_GC'GG Cuts at: 0 567 720 Size: 567 153 SmaI CCC'GGG Cuts at: 0 229 428 645 720 Size: 229 199 217 75 Fragments arranged by size: 229 217 199 75 SrfI GCCC'GGGC Cuts at: 0 645 720 Size: 645 75 StyI C'CwwG_G Cuts at: 0 119 720 Size: 119 601 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 325 720 Size: 325 395 XmaI C'CCGG_G Cuts at: 0 227 426 643 720 Size: 227 199 217 77 Fragments arranged by size: 227 217 199 77 Enzymes that do cut: Acc65I AflIII AlwNI ApaI ApaLI AvaI BamHI BanI BanII BglI BglII Bme1580I BplI BpmI Bpu10I BsaAI BsaHI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstAPI BstEII BstYI BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII KpnI MslI MspA1I NaeI NgoMIV NspI PciI PmlI PpuMI PshAI PspOMI RsrII SacII SmaI SrfI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PpiI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI