Restriction map for
pFN21AE1541
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BcnI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 538 GTAGAC GT^[AC][GT]AC
AccII 774 CGCG CG^CG
AflIII 816 ACATGT A^C[GA][CT]GT
AlwI 679 GGATC GGATC(4/5)
AseI 781 ATTAAT AT^TAAT
AsnI 781 ATTAAT AT^TAAT
AspHI 251 GAGCTC G[AT]GC[AT]^C
AspI 541 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 315 GGCACC G^G[CT][GA]CC
BanII 251 GAGCTC G[GA]GC[CT]^C
BbsI 834 GAAGAC GAAGAC(2/6)
BcgI 136 GCATTTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 410 GTATCC GTATCC(6/5)
BglI 853 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 761 GCTCAGC GC^T[ACGT]AGC
BmyI 251 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 761 GCTCAGC GC^T[ACGT]AGC
BpuAI 834 GAAGAC GAAGAC(2/6)
BsaWI 607 ACCGGA [AT]^CCGG[AT]
BsiHKAI 251 GAGCTC G[AT]GC[AT]^C
BsmBI 200 GAGACG CGTCTC(1/5)
Bsp1286I 251 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 816 ACATGT A^CATGT
BsrDI 1079 CATTGC GCAATG(2/0)
BstEII 1398 GGTCACC G^GT[ACGT]ACC
BstPI 1398 GGTCACC G^GT[ACGT]ACC
BstUI 774 CGCG CG^CG
BstXI 605 CCACCGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 761 GCTCAGC GC^T[ACGT]AGC
CfoI 775 GCGC GCG^C
DsaI 84 CCGTGG C^C[GA][CT]GG
EcoO65I 1398 GGTCACC G^GT[ACGT]ACC
FauI 758 CCCGC CCCGC(4/6)
HhaI 775 GCGC GCG^C
HinP1I 775 GCGC G^CGC
MslI 1401 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 774 CGCG CG^CG
NdeI 125 CATATG CA^TATG
PflFI 541 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 414 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 684 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 781 ATTAAT AT^TAAT
PstI 577 CTGCAG CTGCA^G
SacI 251 GAGCTC GAGCT^C
ScaI 170 AGTACT AGT^ACT
SexAI 1016 ACCTGGT A^CC[AT]GGT
SfaNI 475 GATGC GCATC(5/9)
TaqI 1145 TCGA T^CGA
Tth111I 541 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1145 TCGA T^CGA
Van91I 414 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 1266 GAATTC [GA]^AATT[CT]
1348 GAATTC
AlwNI 415 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1355 CAGTTCCTG
ApoI 1266 GAATTC [GA]^AATT[CT]
1348 GAATTC
BseRI 689 CTCCTC GAGGAG(10/8)
713 CTCCTC
BsmAI 200 GAGAC GTCTC(1/5)
724 GTCTC
BsrBI 759 CCGCTC CCGCTC(-3/-3)
651 GAGCGG
BstF5I 926 CATCC GGATG(2/0)
1185 GGATG
EcoNI 697 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1324 CCTACAGAAGG
EcoRI 1266 GAATTC G^AATTC
1348 GAATTC
FokI 926 CATCC GGATG(9/13)
1185 GGATG
HapII 608 CCGG C^CGG
677 CCGG
HincII 278 GTCAAC GT[CT]^[GA]AC
1069 GTCAAC
HindII 278 GTCAAC GT[CT]^[GA]AC
1069 GTCAAC
HindIII 298 AAGCTT A^AGCTT
551 AAGCTT
HpaII 608 CCGG C^CGG
677 CCGG
MaeII 203 ACGT A^CGT
1151 ACGT
MspA1I 395 CAGCTG C[AC]G^C[GT]G
956 CAGCTG
MspI 608 CCGG C^CGG
677 CCGG
PvuII 395 CAGCTG CAG^CTG
956 CAGCTG
SfcI 577 CTGCAG C^T[GA][CT]AG
1325 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsgI BsiYI BslI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp45I Tsp509I
TspRI