(Circular) (Six-base) MAPSORT of: pFN21AE1541.seq Check: 2983 from: 1 to: 6130 pFN21AE1541 6130 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 3473 1937 Size: 607 929 4594 Fragments arranged by size: 4594 929 607 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2882 4193 5577 5845 1632 Size: 1250 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1250 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2821 5029 6006 1203 Size: 1618 2208 977 1327 Fragments arranged by size: 2208 1618 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 3410 1166 Size: 2244 3886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2235 1184 Size: 1051 5079 AlwNI CAG_nnn'CTG Cuts at: 2425 3365 3873 5445 2425 Size: 940 508 1572 3110 Fragments arranged by size: 3110 1572 940 508 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3271 3353 3439 3665 3817 5926 1493 Size: 1778 82 86 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 86 82 AseI AT'TA_AT Cuts at: 160 1032 2787 4684 160 Size: 872 1755 1897 1606 Fragments arranged by size: 1897 1755 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 3451 4906 618 Size: 325 217 627 533 1131 1455 1842 Fragments arranged by size: 1842 1455 1131 627 533 325 217 BanII G_rGCy'C Cuts at: 1391 2260 3449 1391 Size: 869 1189 4072 Fragments arranged by size: 4072 1189 869 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2846 928 Size: 971 947 4212 Fragments arranged by size: 4212 971 947 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2130 2164 4321 4355 1903 Size: 34 193 34 2157 34 3678 Fragments arranged by size: 3678 2157 193 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2426 4022 5232 2426 Size: 1596 1210 3324 Fragments arranged by size: 3324 1596 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2864 4741 136 Size: 107 122 71 631 605 1192 1877 1525 Fragments arranged by size: 1877 1525 1192 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 2767 3547 2767 Size: 780 5350 Bme1580I G_kGCm'C Cuts at: 1790 4191 5347 1790 Size: 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 BmgBI CAC'GTC Cuts at: 1318 2209 3157 1318 Size: 891 948 4291 Fragments arranged by size: 4291 948 891 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2557 2720 3353 Size: 416 53 83 399 24 1140 163 633 Cuts at: 3353 3861 4819 442 Size: 508 958 1753 Fragments arranged by size: 1753 1140 958 633 508 416 399 163 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2644 2996 3447 4790 1239 Size: 839 566 352 451 1343 2579 Fragments arranged by size: 2579 1343 839 566 451 352 Bpu10I CC'TnA_GC Cuts at: 3263 3366 3263 Size: 103 6027 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2612 4563 4961 5235 5382 5793 2612 Size: 1951 398 274 147 411 2949 Fragments arranged by size: 2949 1951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2685 2709 1186 Size: 1499 24 4607 Fragments arranged by size: 4607 1499 24 BseYI C'CCAG_C Cuts at: 2430 5333 5829 2430 Size: 2903 496 2731 Fragments arranged by size: 2903 2731 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2231 2892 1163 Size: 573 420 75 661 4401 Fragments arranged by size: 4401 661 573 420 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2260 4191 4276 5347 2260 Size: 1931 85 1071 3043 Fragments arranged by size: 3043 1931 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 BsmBI CGTCTCn'nnnn_ Cuts at: 2199 2199 Size: 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2260 3449 4191 4276 5347 1391 Size: 399 470 1189 742 85 1071 2174 Fragments arranged by size: 2174 1189 1071 742 470 399 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2658 2766 4017 1394 Size: 429 835 108 1251 3507 Fragments arranged by size: 3507 1251 835 429 108 BsrDI GCAATG_nn' Cuts at: 65 1248 3083 4625 4799 65 Size: 1183 1835 1542 174 1396 Fragments arranged by size: 1835 1542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 3403 1184 Size: 2219 3911 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2617 Size: 6130 BstYI r'GATC_y Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125 Size: 1488 367 385 17 1452 86 12 346 Cuts at: 6125 1972 Size: 1977 Fragments arranged by size: 1977 1488 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2089 513 Size: 552 939 85 4554 Fragments arranged by size: 4554 939 552 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2893 3646 4440 4460 2052 Size: 841 753 794 20 3722 Fragments arranged by size: 3722 841 794 753 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4060 1353 Size: 2707 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2258 2258 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2644 2882 2996 3447 4193 4790 Size: 393 446 566 238 114 451 746 597 Cuts at: 4790 5577 5845 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 746 597 566 451 446 393 268 238 114 EcoNI CCTnn'n_nnAGG Cuts at: 2706 3333 2706 Size: 627 5503 EcoO109I rG'GnC_Cy Cuts at: 2422 2636 2690 2711 2422 Size: 214 54 21 5841 Fragments arranged by size: 5841 214 54 21 EcoRI G'AATT_C Cuts at: 3271 3353 3439 3817 3271 Size: 82 86 378 5584 Fragments arranged by size: 5584 378 86 82 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 4771 4803 4845 Size: 32 135 32 479 32 2688 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2688 2626 479 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2285 3076 3474 3720 669 Size: 988 281 347 791 398 246 3079 Fragments arranged by size: 3079 988 791 398 347 281 246 HindIII A'AGCT_T Cuts at: 748 2303 2556 748 Size: 1555 253 4322 Fragments arranged by size: 4322 1555 253 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2229 3042 5244 5428 1305 Size: 924 813 2202 184 2007 Fragments arranged by size: 2202 2007 924 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3410 4088 4447 4606 518 Size: 648 2244 678 359 159 2042 Fragments arranged by size: 2244 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2963 3546 4223 5371 5616 1606 Size: 796 561 583 677 1148 245 2120 Fragments arranged by size: 2120 1148 796 677 583 561 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 2131 387 Size: 1744 4386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2017 2086 2222 2543 2813 2825 3488 Size: 97 788 69 136 321 270 12 663 Cuts at: 3488 5033 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 788 663 321 270 136 97 69 12 PasI CC'CwG_GG Cuts at: 1348 2425 1348 Size: 1077 5053 PciI A'CATG_T Cuts at: 2821 5029 2821 Size: 2208 3922 PflMI CCAn_nnn'nTGG Cuts at: 1108 2425 1108 Size: 1317 4813 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2690 2690 Size: 6130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2586 3482 830 Size: 1756 896 3478 Fragments arranged by size: 3478 1756 896 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2402 2963 2402 Size: 561 5569 SacI G_AGCT'C Cuts at: 2260 2260 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 2177 4378 6090 2177 Size: 2201 1712 2217 Fragments arranged by size: 2217 2201 1712 SexAI A'CCwGG_T Cuts at: 3021 3021 Size: 6130 SfcI C'TryA_G Cuts at: 826 1045 2582 3330 3478 4613 5294 5485 826 Size: 219 1537 748 148 1135 681 191 1471 Fragments arranged by size: 1537 1471 1135 748 681 219 191 148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 3569 513 Size: 552 939 1565 3074 Fragments arranged by size: 3074 1565 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2338 2422 4157 4174 4327 4512 4957 Size: 1255 758 84 1735 17 153 185 445 Cuts at: 4957 325 Size: 1498 Fragments arranged by size: 1735 1498 1255 758 445 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 2517 2965 Size: 274 80 33 51 1443 198 342 448 Cuts at: 2965 4376 6088 96 Size: 1411 1712 138 Fragments arranged by size: 1712 1443 1411 448 342 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 2549 1316 Size: 1233 4897 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI