(Linear) (Six-base) MAPSORT of: KIEE1541.seq Check: 1618 from: 1 to: 1425 KIEE1541.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AccI GT'mk_AC Cuts at: 0 539 1425 Size: 539 886 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 1425 Size: 877 548 AflIII A'CryG_T Cuts at: 0 816 1425 Size: 816 609 AleI CACnn'nnGTG Cuts at: 0 1405 1425 Size: 1405 20 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 230 1425 Size: 230 1195 AlwNI CAG_nnn'CTG Cuts at: 0 420 1360 1425 Size: 420 940 65 Fragments arranged by size: 940 420 65 ApoI r'AATT_y Cuts at: 0 1266 1348 1425 Size: 1266 82 77 Fragments arranged by size: 1266 82 77 AseI AT'TA_AT Cuts at: 0 782 1425 Size: 782 643 BanI G'GyrC_C Cuts at: 0 315 1425 Size: 315 1110 BanII G_rGCy'C Cuts at: 0 255 1425 Size: 255 1170 BbsI GAAGACnn'nnnn_ Cuts at: 0 841 1425 Size: 841 584 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 125 159 1425 Size: 125 34 1266 Fragments arranged by size: 1266 125 34 BciVI GTATCCnnnnn_n' Cuts at: 0 421 1425 Size: 421 1004 BglI GCCn_nnn'nGGC Cuts at: 0 859 1425 Size: 859 566 BlpI GC'TnA_GC Cuts at: 0 762 1425 Size: 762 663 BmgBI CAC'GTC Cuts at: 0 204 1152 1425 Size: 204 948 273 Fragments arranged by size: 948 273 204 BmrI ACTGGGnnnn_n' Cuts at: 0 552 715 1348 1425 Size: 552 163 633 77 Fragments arranged by size: 633 552 163 77 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 639 991 1425 Size: 73 566 352 434 Fragments arranged by size: 566 434 352 73 Bpu10I CC'TnA_GC Cuts at: 0 1258 1361 1425 Size: 1258 103 64 Fragments arranged by size: 1258 103 64 BsaWI w'CCGG_w Cuts at: 0 607 1425 Size: 607 818 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 680 704 1425 Size: 680 24 721 Fragments arranged by size: 721 680 24 BseYI C'CCAG_C Cuts at: 0 425 1425 Size: 425 1000 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 226 887 1425 Size: 151 75 661 538 Fragments arranged by size: 661 538 151 75 BsiHKAI G_wGCw'C Cuts at: 0 255 1425 Size: 255 1170 BsmBI CGTCTCn'nnnn_ Cuts at: 0 194 1425 Size: 194 1231 Bsp1286I G_dGCh'C Cuts at: 0 255 1425 Size: 255 1170 BsrBI CCG'CTC Cuts at: 0 653 761 1425 Size: 653 108 664 Fragments arranged by size: 664 653 108 BsrDI GCAATG_nn' Cuts at: 0 1078 1425 Size: 1078 347 BstEII G'GTnAC_C Cuts at: 0 1398 1425 Size: 1398 27 BstXI CCAn_nnnn'nTGG Cuts at: 0 612 1425 Size: 612 813 BtgI C'CryG_G Cuts at: 0 84 1425 Size: 84 1341 BtsI GCAGTG_nn' Cuts at: 0 47 888 1425 Size: 47 841 537 Fragments arranged by size: 841 537 47 EcoICRI GAG'CTC Cuts at: 0 253 1425 Size: 253 1172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 639 877 991 1425 Size: 73 566 238 114 434 Fragments arranged by size: 566 434 238 114 73 EcoNI CCTnn'n_nnAGG Cuts at: 0 701 1328 1425 Size: 701 627 97 Fragments arranged by size: 701 627 97 EcoO109I rG'GnC_Cy Cuts at: 0 417 631 685 706 1425 Size: 417 214 54 21 719 Fragments arranged by size: 719 417 214 54 21 EcoRI G'AATT_C Cuts at: 0 1266 1348 1425 Size: 1266 82 77 Fragments arranged by size: 1266 82 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 1425 Size: 46 32 1347 Fragments arranged by size: 1347 46 32 HincII GTy'rAC Cuts at: 0 280 1071 1425 Size: 280 791 354 Fragments arranged by size: 791 354 280 HindIII A'AGCT_T Cuts at: 0 298 551 1425 Size: 298 253 874 Fragments arranged by size: 874 298 253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 224 1037 1425 Size: 224 813 388 Fragments arranged by size: 813 388 224 MslI CAynn'nnrTG Cuts at: 0 1405 1425 Size: 1405 20 MspA1I CmG'CkG Cuts at: 0 397 958 1425 Size: 397 561 467 Fragments arranged by size: 561 467 397 NdeI CA'TA_TG Cuts at: 0 126 1425 Size: 126 1299 NspI r_CATG'y Cuts at: 0 12 81 217 538 808 820 1425 Size: 12 69 136 321 270 12 605 Fragments arranged by size: 605 321 270 136 69 12 12 PasI CC'CwG_GG Cuts at: 0 420 1425 Size: 420 1005 PciI A'CATG_T Cuts at: 0 816 1425 Size: 816 609 PflMI CCAn_nnn'nTGG Cuts at: 0 420 1425 Size: 420 1005 PpuMI rG'GwC_Cy Cuts at: 0 685 1425 Size: 685 740 PstI C_TGCA'G Cuts at: 0 581 1425 Size: 581 844 PvuII CAG'CTG Cuts at: 0 397 958 1425 Size: 397 561 467 Fragments arranged by size: 561 467 397 SacI G_AGCT'C Cuts at: 0 255 1425 Size: 255 1170 ScaI AGT'ACT Cuts at: 0 172 1425 Size: 172 1253 SexAI A'CCwGG_T Cuts at: 0 1016 1425 Size: 1016 409 SfcI C'TryA_G Cuts at: 0 577 1325 1425 Size: 577 748 100 Fragments arranged by size: 748 577 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 333 417 1425 Size: 333 84 1008 Fragments arranged by size: 1008 333 84 TatI w'GTAC_w Cuts at: 0 170 512 960 1425 Size: 170 342 448 465 Fragments arranged by size: 465 448 342 170 Tth111I GACn'n_nGTC Cuts at: 0 544 1425 Size: 544 881 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI BanI BanII BbsI BcgI BciVI BglI BlpI BmgBI BmrI BpmI Bpu10I BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BstEII BstXI BtgI BtsI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI MspA1I NdeI NspI PasI PciI PflMI PpuMI PstI PvuII SacI ScaI SexAI SfcI TaqII TatI Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII Bme1580I BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI