(Circular) (Six-base) MAPSORT of: pFN21AB6608.seq Check: 5805 from: 1 to: 5998 pFN21AB6608 5998 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2401 2853 2401 Size: 452 5546 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 3228 3341 1937 Size: 778 513 113 4594 Fragments arranged by size: 4594 778 513 113 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2324 2861 3084 3230 4061 5445 5713 Size: 656 36 537 223 146 831 1384 268 Cuts at: 5713 1632 Size: 1917 Fragments arranged by size: 1917 1384 831 656 537 268 223 146 36 AfeI AGC'GCT Cuts at: 2803 2803 Size: 5998 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 2117 2625 4897 5874 1203 Size: 914 508 2272 977 1327 Fragments arranged by size: 2272 1327 977 914 508 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 3143 4727 3143 Size: 1584 4414 AleI CACnn'nnGTG Cuts at: 1166 2624 1166 Size: 1458 4540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AlwNI CAG_nnn'CTG Cuts at: 3741 5313 3741 Size: 1572 4426 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 2171 2498 2552 2968 3307 3533 3685 5794 Size: 678 327 54 416 339 226 152 2109 Cuts at: 5794 1493 Size: 1697 Fragments arranged by size: 2109 1697 678 416 339 327 226 152 54 AscI GG'CGCG_CC Cuts at: 2021 2021 Size: 5998 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3323 1870 Size: 73 1380 4545 Fragments arranged by size: 4545 1380 73 BamHI G'GATC_C Cuts at: 3258 3328 3695 3258 Size: 70 367 5561 Fragments arranged by size: 5561 367 70 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2601 3319 4774 618 Size: 325 217 627 814 718 1455 1842 Fragments arranged by size: 1842 1455 814 718 627 325 217 BanII G_rGCy'C Cuts at: 1391 2014 2615 3317 1391 Size: 623 601 702 4072 Fragments arranged by size: 4072 702 623 601 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2382 2416 4189 4223 1903 Size: 34 445 34 1773 34 3678 Fragments arranged by size: 3678 1773 445 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 2661 1548 Size: 1113 4885 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 4609 136 Size: 107 122 71 631 605 346 2591 1525 Fragments arranged by size: 2591 1525 631 605 346 122 107 71 BglII A'GATC_T Cuts at: 2327 5993 2327 Size: 3666 2332 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 3208 4059 5215 1790 Size: 1418 851 1156 2573 Fragments arranged by size: 2573 1418 1156 851 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2920 3729 4687 Size: 416 53 83 399 24 1503 809 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1503 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2657 2657 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2469 4658 1239 Size: 1230 2189 2579 Fragments arranged by size: 2579 2189 1230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2713 4123 4988 5286 2713 Size: 1410 865 298 3425 Fragments arranged by size: 3425 1410 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2626 3789 493 Size: 711 1422 1163 2702 Fragments arranged by size: 2702 1422 1163 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3225 3805 4187 4822 Size: 53 83 186 936 1692 580 382 635 Cuts at: 4822 275 Size: 1451 Fragments arranged by size: 1692 1451 936 635 580 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2385 2989 4431 4829 5103 5250 5661 2385 Size: 604 1442 398 274 147 411 2722 Fragments arranged by size: 2722 1442 604 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2747 2777 2849 2879 1178 Size: 30 165 30 1344 30 72 30 4297 Fragments arranged by size: 4297 1344 165 72 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2467 2472 1186 Size: 1281 5 4712 Fragments arranged by size: 4712 1281 5 BseYI C'CCAG_C Cuts at: 2344 2655 3189 5201 5697 2344 Size: 311 534 2012 496 2645 Fragments arranged by size: 2645 2012 534 496 311 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 1163 Size: 573 693 4732 Fragments arranged by size: 4732 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4059 4144 5215 4059 Size: 85 1071 4842 Fragments arranged by size: 4842 1071 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2615 3208 3317 4059 4144 5215 Size: 399 224 601 593 109 742 85 1071 Cuts at: 5215 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 601 593 399 224 109 85 BspEI T'CCGG_A Cuts at: 2989 5661 2989 Size: 2672 3326 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2219 2401 2853 3353 844 Size: 1375 182 452 500 3489 Fragments arranged by size: 3489 1375 500 452 182 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2074 2829 4493 4667 65 Size: 1183 826 755 1664 174 1396 Fragments arranged by size: 1664 1396 1183 826 755 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2268 4642 4829 1700 Size: 110 458 2374 187 2869 Fragments arranged by size: 2869 2374 458 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 2021 1925 Size: 96 5902 BssSI C'ACGA_G Cuts at: 3232 4058 5070 3232 Size: 826 1012 4160 Fragments arranged by size: 4160 1012 826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2327 2844 3258 3328 3695 4080 4097 5549 Size: 355 517 414 70 367 385 17 1452 Cuts at: 5549 5635 5647 5993 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 517 414 385 367 355 346 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2262 3196 513 Size: 552 1197 934 3315 Fragments arranged by size: 3315 1197 934 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 2674 3514 4308 4328 2674 Size: 840 794 20 4344 Fragments arranged by size: 4344 840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2711 5005 809 Size: 507 1395 2294 1802 Fragments arranged by size: 2294 1802 1395 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2509 2586 3445 Size: 54 1425 50 275 18 679 77 859 Cuts at: 3445 4334 5963 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 859 679 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2343 3273 3928 1353 Size: 990 930 655 3423 Fragments arranged by size: 3423 990 930 655 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2324 2469 2861 3084 3230 4061 Size: 393 656 36 145 392 223 146 831 Cuts at: 4061 4658 5445 5713 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 831 787 656 597 393 392 268 223 146 145 36 EcoNI CCTnn'n_nnAGG Cuts at: 2789 2789 Size: 5998 EcoO109I rG'GnC_Cy Cuts at: 2526 3137 2526 Size: 611 5387 EcoRI G'AATT_C Cuts at: 2552 2968 3307 3685 2552 Size: 416 339 378 4865 Fragments arranged by size: 4865 416 378 339 FseI GG_CCGG'CC Cuts at: 2272 2272 Size: 5998 FspI TGC'GCA Cuts at: 2458 2871 4504 2458 Size: 413 1633 3952 Fragments arranged by size: 3952 1633 413 HaeII r_GCGC'y Cuts at: 1678 2670 2805 5145 1678 Size: 992 135 2340 2531 Fragments arranged by size: 2531 2340 992 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3129 3161 3250 3282 4639 Size: 32 135 32 1557 32 89 32 1357 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1557 1357 135 89 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2151 2166 2766 5112 5296 1305 Size: 846 15 600 2346 184 2007 Fragments arranged by size: 2346 2007 846 600 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2511 10 Size: 54 1425 343 679 3497 Fragments arranged by size: 3497 1425 679 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 3077 3956 4315 4474 518 Size: 648 1458 453 879 359 159 2042 Fragments arranged by size: 2042 1458 879 648 453 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 2998 3414 4091 5239 5484 1606 Size: 738 654 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 654 416 245 NaeI GCC'GGC Cuts at: 2270 2270 Size: 5998 NcoI C'CATG_G Cuts at: 513 1065 2262 513 Size: 552 1197 4249 Fragments arranged by size: 4249 1197 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2268 2268 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2121 3356 4901 1132 Size: 97 892 1235 1545 2229 Fragments arranged by size: 2229 1545 1235 892 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 2117 4897 2117 Size: 2780 3218 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PfoI T'CCnGG_A Cuts at: 2879 2879 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3137 3137 Size: 5998 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PstI C_TGCA'G Cuts at: 830 2372 3350 830 Size: 1542 978 3478 Fragments arranged by size: 3478 1542 978 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2344 2998 2344 Size: 654 5344 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SanDI GG'GwC_CC Cuts at: 3137 3137 Size: 5998 SapI GCTCTTCn'nnn_ Cuts at: 2343 2343 Size: 5998 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 2135 4246 5958 2135 Size: 2111 1712 2175 Fragments arranged by size: 2175 2111 1712 SfcI C'TryA_G Cuts at: 826 1045 2368 2593 2797 3346 4481 5162 5353 Size: 219 1323 225 204 549 1135 681 191 Cuts at: 5353 826 Size: 1471 Fragments arranged by size: 1471 1323 1135 681 549 225 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2728 4102 5003 5265 820 Size: 197 926 785 1374 901 262 1553 Fragments arranged by size: 1553 1374 926 901 785 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2262 2853 3157 3273 3437 513 Size: 552 1197 591 304 116 164 3074 Fragments arranged by size: 3074 1197 591 552 304 164 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 4025 4042 4195 4380 4825 325 Size: 1255 1067 1378 17 153 185 445 1498 Fragments arranged by size: 1498 1378 1255 1067 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2133 4244 5956 Size: 274 80 33 51 1443 156 2111 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 2111 1712 1443 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2106 2138 2849 2881 5958 5990 2106 Size: 32 711 32 3077 32 2114 Fragments arranged by size: 3077 2114 711 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI