(Linear) (Six-base) MAPSORT of: KIEE4581.seq Check: 2802 from: 1 to: 2415 KIEE4581.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1227 2415 Size: 1227 1188 AatII G_ACGT'C Cuts at: 0 1787 2415 Size: 1787 628 AccI GT'mk_AC Cuts at: 0 1487 2415 Size: 1487 928 Acc65I G'GTAC_C Cuts at: 0 2366 2415 Size: 2366 49 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2143 2415 Size: 2143 272 AfeI AGC'GCT Cuts at: 0 376 2415 Size: 376 2039 AflIII A'CryG_T Cuts at: 0 1043 2174 2415 Size: 1043 1131 241 Fragments arranged by size: 1131 1043 241 AhdI GACnn_n'nnGTC Cuts at: 0 1962 2415 Size: 1962 453 AleI CACnn'nnGTG Cuts at: 0 1900 2415 Size: 1900 515 AlwNI CAG_nnn'CTG Cuts at: 0 877 966 1320 2031 2415 Size: 877 89 354 711 384 Fragments arranged by size: 877 711 384 354 89 ApaI G_GGCC'C Cuts at: 0 2289 2415 Size: 2289 126 ApaLI G'TGCA_C Cuts at: 0 2196 2415 Size: 2196 219 ApoI r'AATT_y Cuts at: 0 1014 2415 Size: 1014 1401 BamHI G'GATC_C Cuts at: 0 401 2415 Size: 401 2014 BanI G'GyrC_C Cuts at: 0 60 2111 2241 2300 2366 2415 Size: 60 2051 130 59 66 49 Fragments arranged by size: 2051 130 66 60 59 49 BanII G_rGCy'C Cuts at: 0 571 909 1333 1520 2289 2399 2415 Size: 571 338 424 187 769 110 16 Fragments arranged by size: 769 571 424 338 187 110 16 BbeI G_GCGC'C Cuts at: 0 64 2115 2415 Size: 64 2051 300 Fragments arranged by size: 2051 300 64 BbsI GAAGACnn'nnnn_ Cuts at: 0 1122 1530 1779 2415 Size: 1122 408 249 636 Fragments arranged by size: 1122 636 408 249 BclI T'GATC_A Cuts at: 0 683 2415 Size: 683 1732 BglI GCCn_nnn'nGGC Cuts at: 0 1270 2019 2415 Size: 1270 749 396 Fragments arranged by size: 1270 749 396 BglII A'GATC_T Cuts at: 0 821 2415 Size: 821 1594 BlpI GC'TnA_GC Cuts at: 0 1951 2415 Size: 1951 464 Bme1580I G_kGCm'C Cuts at: 0 1835 2200 2246 2289 2415 Size: 1835 365 46 43 126 Fragments arranged by size: 1835 365 126 46 43 BmgBI CAC'GTC Cuts at: 0 1947 2415 Size: 1947 468 BmrI ACTGGGnnnn_n' Cuts at: 0 1854 2415 Size: 1854 561 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1395 2415 Size: 1395 1020 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 993 2008 2415 Size: 343 650 1015 407 Fragments arranged by size: 1015 650 407 343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 445 655 2415 Size: 259 186 210 1760 Fragments arranged by size: 1760 259 210 186 BsaAI yAC'GTr Cuts at: 0 660 2175 2415 Size: 660 1515 240 Fragments arranged by size: 1515 660 240 BsaHI Gr'CG_yC Cuts at: 0 61 100 1784 1934 1963 2112 2138 2254 Size: 61 39 1684 150 29 149 26 116 Cuts at: 2254 2415 Size: 161 Fragments arranged by size: 1684 161 150 149 116 61 39 29 26 BsaWI w'CCGG_w Cuts at: 0 356 694 2415 Size: 356 338 1721 Fragments arranged by size: 1721 356 338 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 850 880 896 926 931 961 2415 Size: 850 30 16 30 5 30 1454 Fragments arranged by size: 1454 850 30 30 30 16 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 67 340 343 952 2407 2415 Size: 67 273 3 609 1455 8 Fragments arranged by size: 1455 609 273 67 8 3 BseYI C'CCAG_C Cuts at: 0 118 377 1661 1665 2009 2045 2415 Size: 118 259 1284 4 344 36 370 Fragments arranged by size: 1284 370 344 259 118 36 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1382 1825 2415 Size: 1382 443 590 Fragments arranged by size: 1382 590 443 BsiEI CG_ry'CG Cuts at: 0 36 884 2415 Size: 36 848 1531 Fragments arranged by size: 1531 848 36 BsiHKAI G_wGCw'C Cuts at: 0 563 1760 1897 2200 2399 2415 Size: 563 1197 137 303 199 16 Fragments arranged by size: 1197 563 303 199 137 16 BsmBI CGTCTCn'nnnn_ Cuts at: 0 15 870 2129 2415 Size: 15 855 1259 286 Fragments arranged by size: 1259 855 286 15 Bsp1286I G_dGCh'C Cuts at: 0 563 571 909 1333 1520 1760 1835 1897 Size: 563 8 338 424 187 240 75 62 Cuts at: 1897 2200 2246 2289 2399 2415 Size: 303 46 43 110 16 Fragments arranged by size: 563 424 338 303 240 187 110 75 62 46 43 16 8 BspEI T'CCGG_A Cuts at: 0 356 2415 Size: 356 2059 BspHI T'CATG_A Cuts at: 0 1706 1760 2219 2415 Size: 1706 54 459 196 Fragments arranged by size: 1706 459 196 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1227 1915 2415 Size: 1227 688 500 Fragments arranged by size: 1227 688 500 BsrBI CCG'CTC Cuts at: 0 333 2085 2190 2415 Size: 333 1752 105 225 Fragments arranged by size: 1752 333 225 105 BsrFI r'CCGG_y Cuts at: 0 594 1223 1574 2415 Size: 594 629 351 841 Fragments arranged by size: 841 629 594 351 BssHII G'CGCG_C Cuts at: 0 133 2415 Size: 133 2282 BssSI C'ACGA_G Cuts at: 0 415 562 1117 2415 Size: 415 147 555 1298 Fragments arranged by size: 1298 555 415 147 BstAPI GCAn_nnn'nTGC Cuts at: 0 877 1830 2415 Size: 877 953 585 Fragments arranged by size: 953 877 585 BstBI TT'CG_AA Cuts at: 0 1206 2415 Size: 1206 1209 BstEII G'GTnAC_C Cuts at: 0 1857 2343 2415 Size: 1857 486 72 Fragments arranged by size: 1857 486 72 BstXI CCAn_nnnn'nTGG Cuts at: 0 1146 1752 1776 2415 Size: 1146 606 24 639 Fragments arranged by size: 1146 639 606 24 BstYI r'GATC_y Cuts at: 0 245 401 650 779 821 977 1121 1586 Size: 245 156 249 129 42 156 144 465 Cuts at: 1586 2415 Size: 829 Fragments arranged by size: 829 465 249 245 156 156 144 129 42 Bsu36I CC'TnA_GG Cuts at: 0 1912 2116 2415 Size: 1912 204 299 Fragments arranged by size: 1912 299 204 BtgI C'CryG_G Cuts at: 0 710 728 911 1035 1139 1382 1985 2201 Size: 710 18 183 124 104 243 603 216 Cuts at: 2201 2263 2415 Size: 62 152 Fragments arranged by size: 710 603 243 216 183 152 124 104 62 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1119 2415 Size: 1119 1296 BtsI GCAGTG_nn' Cuts at: 0 1175 1874 2415 Size: 1175 699 541 Fragments arranged by size: 1175 699 541 DraIII CAC_nnn'GTG Cuts at: 0 663 2073 2307 2415 Size: 663 1410 234 108 Fragments arranged by size: 1410 663 234 108 EaeI y'GGCC_r Cuts at: 0 2 33 725 881 1259 1271 2415 Size: 2 31 692 156 378 12 1144 Fragments arranged by size: 1144 692 378 156 31 12 2 EagI C'GGCC_G Cuts at: 0 33 881 2415 Size: 33 848 1534 Fragments arranged by size: 1534 848 33 EarI CTCTTCn'nnn_ Cuts at: 0 1408 1435 2415 Size: 1408 27 980 Fragments arranged by size: 1408 980 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 351 524 1639 2415 Size: 351 173 1115 776 Fragments arranged by size: 1115 776 351 173 EcoICRI GAG'CTC Cuts at: 0 2397 2415 Size: 2397 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 993 2008 2143 2415 Size: 343 650 1015 135 272 Fragments arranged by size: 1015 650 343 272 135 EcoNI CCTnn'n_nnAGG Cuts at: 0 325 1911 2415 Size: 325 1586 504 Fragments arranged by size: 1586 504 325 EcoO109I rG'GnC_Cy Cuts at: 0 720 921 1050 1797 1851 2028 2286 2355 Size: 720 201 129 747 54 177 258 69 Cuts at: 2355 2415 Size: 60 Fragments arranged by size: 747 720 258 201 177 129 69 60 54 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 876 2403 2415 Size: 876 1527 12 Fragments arranged by size: 1527 876 12 HaeII r_GCGC'y Cuts at: 0 64 378 2115 2415 Size: 64 314 1737 300 Fragments arranged by size: 1737 314 300 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 850 882 931 963 1948 1980 2415 Size: 850 32 49 32 985 32 435 Fragments arranged by size: 985 850 435 49 32 32 32 HincII GTy'rAC Cuts at: 0 797 2415 Size: 797 1618 KasI G'GCGC_C Cuts at: 0 60 2111 2415 Size: 60 2051 304 Fragments arranged by size: 2051 304 60 KpnI G_GTAC'C Cuts at: 0 2370 2415 Size: 2370 45 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 147 2114 2415 Size: 147 1967 301 Fragments arranged by size: 1967 301 147 MscI TGG'CCA Cuts at: 0 4 2415 Size: 4 2411 MslI CAynn'nnrTG Cuts at: 0 439 1138 1144 1708 1753 1900 2000 2173 Size: 439 699 6 564 45 147 100 173 Cuts at: 2173 2415 Size: 242 Fragments arranged by size: 699 564 439 242 173 147 100 45 6 MspA1I CmG'CkG Cuts at: 0 66 105 315 611 895 1347 1665 1821 Size: 66 39 210 296 284 452 318 156 Cuts at: 1821 2109 2415 Size: 288 306 Fragments arranged by size: 452 318 306 296 288 284 210 156 66 39 NaeI GCC'GGC Cuts at: 0 1576 2415 Size: 1576 839 NarI GG'CG_CC Cuts at: 0 61 2112 2415 Size: 61 2051 303 Fragments arranged by size: 2051 303 61 NcoI C'CATG_G Cuts at: 0 1139 1382 2201 2415 Size: 1139 243 819 214 Fragments arranged by size: 1139 819 243 214 NdeI CA'TA_TG Cuts at: 0 173 2415 Size: 173 2242 NgoMIV G'CCGG_C Cuts at: 0 1574 2415 Size: 1574 841 NspI r_CATG'y Cuts at: 0 1047 1812 1999 2415 Size: 1047 765 187 416 Fragments arranged by size: 1047 765 416 187 PasI CC'CwG_GG Cuts at: 0 2289 2415 Size: 2289 126 PciI A'CATG_T Cuts at: 0 1043 2415 Size: 1043 1372 PfoI T'CCnGG_A Cuts at: 0 320 2415 Size: 320 2095 PmlI CAC'GTG Cuts at: 0 660 2175 2415 Size: 660 1515 240 Fragments arranged by size: 1515 660 240 PpuMI rG'GwC_Cy Cuts at: 0 921 1050 2355 2415 Size: 921 129 1305 60 Fragments arranged by size: 1305 921 129 60 PspOMI G'GGCC_C Cuts at: 0 2285 2415 Size: 2285 130 PstI C_TGCA'G Cuts at: 0 1238 1325 1352 1826 2415 Size: 1238 87 27 474 589 Fragments arranged by size: 1238 589 474 87 27 PvuII CAG'CTG Cuts at: 0 315 1665 1821 2415 Size: 315 1350 156 594 Fragments arranged by size: 1350 594 315 156 SacI G_AGCT'C Cuts at: 0 2399 2415 Size: 2399 16 SapI GCTCTTCn'nnn_ Cuts at: 0 1408 2415 Size: 1408 1007 SbfI CC_TGCA'GG Cuts at: 0 1238 1325 2415 Size: 1238 87 1090 Fragments arranged by size: 1238 1090 87 ScaI AGT'ACT Cuts at: 0 832 2415 Size: 832 1583 SexAI A'CCwGG_T Cuts at: 0 2303 2415 Size: 2303 112 SfcI C'TryA_G Cuts at: 0 1234 1321 1348 1822 2415 Size: 1234 87 27 474 593 Fragments arranged by size: 1234 593 474 87 27 SfoI GGC'GCC Cuts at: 0 62 2113 2415 Size: 62 2051 302 Fragments arranged by size: 2051 302 62 SmlI C'TyrA_G Cuts at: 0 274 460 670 2415 Size: 274 186 210 1745 Fragments arranged by size: 1745 274 210 186 SphI G_CATG'C Cuts at: 0 1999 2415 Size: 1999 416 StuI AGG'CCT Cuts at: 0 1067 2415 Size: 1067 1348 StyI C'CwwG_G Cuts at: 0 239 1139 1382 2201 2415 Size: 239 900 243 819 214 Fragments arranged by size: 900 819 243 239 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 904 2023 2172 2415 Size: 904 1119 149 243 Fragments arranged by size: 1119 904 243 149 TatI w'GTAC_w Cuts at: 0 464 830 1373 2415 Size: 464 366 543 1042 Fragments arranged by size: 1042 543 464 366 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 218 250 896 928 2415 Size: 218 32 646 32 1487 Fragments arranged by size: 1487 646 218 32 32 Tth111I GACn'n_nGTC Cuts at: 0 862 2415 Size: 862 1553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1146 2097 2370 2415 Size: 1146 951 273 45 Fragments arranged by size: 1146 951 273 45 XmnI GAAnn'nnTTC Cuts at: 0 961 2128 2415 Size: 961 1167 287 Fragments arranged by size: 1167 961 287 ZraI GAC'GTC Cuts at: 0 1785 2415 Size: 1785 630 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI BbsI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI SbfI ScaI SexAI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AclI AflII AgeI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BfrBI BmtI Bpu10I BsaI BsaBI BsiWI BsmI BsrDI BsrGI BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NheI NotI NruI NsiI PacI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI