Restriction map for pFN21AB5482
 (Circular) (Six-base) MAPSORT of: pFN21AB5482.seq  Check: 3347  from: 1  to: 5440



pFN21AB5482  5440 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3250     278 
   Size:         53      83     186     936    1714    2468

  Fragments arranged by size: 

                2468    1714     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2783    1937 
   Size:        846    4594
 
Acc65I G'GTAC_C

Cuts at:   2761    2761 
   Size:       5440
 
AclI AA'CG_TT

Cuts at:   1563    3567    3940    1563 
   Size:       2004     373    3063

  Fragments arranged by size: 

                3063    2004     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3503    4887    5155    1632 
   Size:       1871    1384     268    1917

  Fragments arranged by size: 

                1917    1871    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2201     820 
   Size:        197    1184    4059

  Fragments arranged by size: 

                4059    1184     197
 
AflIII A'CryG_T

Cuts at:   1203    4339    5316    1203 
   Size:       3136     977    1327

  Fragments arranged by size: 

                3136    1327     977
 
AgeI A'CCGG_T

Cuts at:   4271    4271 
   Size:       5440
 
AhdI GACnn_n'nnGTC

Cuts at:   4169    4169 
   Size:       5440
 
AleI CACnn'nnGTG

Cuts at:   1166    2422    1166 
   Size:       1256    4184
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5440
 
AlwNI CAG_nnn'CTG

Cuts at:   3183    4755    3183 
   Size:       1572    3868
 
ApaLI G'TGCA_C

Cuts at:   3497    4653    3497 
   Size:       1156    4284
 
ApoI r'AATT_y

Cuts at:   1493    2429    2749    2975    3127    5236    1493 
   Size:        936     320     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     936     320     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3994     160 
   Size:        872    2962    1606

  Fragments arranged by size: 

                2962    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5440
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2145    2765    1870 
   Size:         73     202     620    4545

  Fragments arranged by size: 

                4545     620     202      73
 
BamHI G'GATC_C

Cuts at:   2770    3137    2770 
   Size:        367    5073
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2276    2761    4216     618 
   Size:        325     217     627     489     485    1455    1842

  Fragments arranged by size: 

                1842    1455     627     489     485     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2759    1391 
   Size:       1368    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2191     928 
   Size:        971     292    4177

  Fragments arranged by size: 

                4177     971     292
 
BbvCI CC'TCA_GC

Cuts at:   2196    2196 
   Size:       5440
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3631    3665    1903 
   Size:         34    1694      34    3678

  Fragments arranged by size: 

                3678    1694      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2384    3332    4542    2384 
   Size:        948    1210    3282

  Fragments arranged by size: 

                3282    1210     948
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5440
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2235    4051 
   Size:        107     122      71     631     605     337     226    1816

Cuts at:   4051     136 
   Size:       1525

  Fragments arranged by size: 

                1816    1525     631     605     337     226     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5435    5435 
   Size:       5440
 
BlpI GC'TnA_GC

Cuts at:   2857    2857 
   Size:       5440
 
Bme1580I G_kGCm'C

Cuts at:   1790    3501    4657    1790 
   Size:       1711    1156    2573

  Fragments arranged by size: 

                2573    1711    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5440
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3171    4129     442 
   Size:        416      53      83     399      24    1754     958    1753

  Fragments arranged by size: 

                1754    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5440
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4100    1239 
   Size:       2861    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2196    2196 
   Size:       5440
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3565    4430    4728    3565 
   Size:        865     298    4277

  Fragments arranged by size: 

                4277     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4103     882 
   Size:        617    2604    2219

  Fragments arranged by size: 

                2604    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3231     493 
   Size:        711    2027    2702

  Fragments arranged by size: 

                2702    2027     711
 
BsaBI GATnn'nnATC

Cuts at:   5434    5434 
   Size:       5440
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3247    3629    4264     275 
   Size:         53      83     186     936    1714     382     635    1451

  Fragments arranged by size: 

                1714    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3873    4271    4545    4692    5103    3873 
   Size:        398     274     147     411    4210

  Fragments arranged by size: 

                4210     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5215

  Fragments arranged by size: 

                5215     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5440
 
BseYI C'CCAG_C

Cuts at:   2096    4643    5139    2096 
   Size:       2547     496    2397

  Fragments arranged by size: 

                2547    2397     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2615    1163 
   Size:        573     879    3988

  Fragments arranged by size: 

                3988     879     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2890    3651    4679    1540 
   Size:        275     186     889     761    1028    2301

  Fragments arranged by size: 

                2301    1028     889     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3501    3586    4657    3501 
   Size:         85    1071    4284

  Fragments arranged by size: 

                4284    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2951    3044    2951 
   Size:         93    5347
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2759    3501    3586    4657    1391 
   Size:        399     969     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     969     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5103    5103 
   Size:       5440
 
BspHI T'CATG_A

Cuts at:   2541    3329    5059    2541 
   Size:        788    1730    2922

  Fragments arranged by size: 

                2922    1730     788
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2795     844 
   Size:       1951    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3327    1394 
   Size:        429    1504    3507

  Fragments arranged by size: 

                3507    1504     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2382    3935    4109      65 
   Size:       1183    1134    1553     174    1396

  Fragments arranged by size: 

                1553    1396    1183    1134     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4084    4271    1700 
   Size:        110    2274     187    2869

  Fragments arranged by size: 

                2869    2274     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2397    2613      96 
   Size:       2301     216    2923

  Fragments arranged by size: 

                2923    2301     216
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5440
 
BssSI C'ACGA_G

Cuts at:   3500    4512    3500 
   Size:       1012    4428
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5440
 
BstBI TT'CG_AA

Cuts at:   4244    4244 
   Size:       5440
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5440
 
BstYI r'GATC_y

Cuts at:   1972    2770    3137    3522    3539    4991    5077    5089    5435 
   Size:        798     367     385      17    1452      86      12     346

Cuts at:   5435    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     798     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3949

  Fragments arranged by size: 

                3949     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4701

  Fragments arranged by size: 

                4701     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2627    2956    3750    3770    2627 
   Size:        329     794      20    4297

  Fragments arranged by size: 

                4297     794     329      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5440
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5405
 
DraI TTT'AAA

Cuts at:   2744    2744 
   Size:       5440
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4447     809 
   Size:        507    3131    1802

  Fragments arranged by size: 

                3131    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2607    2887    3776 
   Size:         54    1425      50     275      18     777     280     889

Cuts at:   3776    5405       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     777     280     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2887    1537 
   Size:        275    1075    4090

  Fragments arranged by size: 

                4090    1075     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2036    2162    2165    3370    1353 
   Size:        683     126       3    1205    3423

  Fragments arranged by size: 

                3423    1205     683     126       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2273    4027    4401    4547     387 
   Size:       1064     138     684    1754     374     146    1280

  Fragments arranged by size: 

                1754    1280    1064     684     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3503    4100    4887    5155    1239 
   Size:        393    1871     597     787     268    1524

  Fragments arranged by size: 

                1871    1524     787     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2667    2667 
   Size:       5440
 
EcoRI G'AATT_C

Cuts at:   2749    3127    2749 
   Size:        378    5062
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2386    2386 
   Size:       5440
 
FspI TGC'GCA

Cuts at:   3946    3946 
   Size:       5440
 
HaeII r_GCGC'y

Cuts at:   1678    4587    1678 
   Size:       2909    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2470    2502    4081    4113    4155 
   Size:         32     135      32     898      32    1579      32      42

Cuts at:   4155    4187    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1579     898     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2784    3030     669 
   Size:        988     281     846     246    3079

  Fragments arranged by size: 

                3079     988     846     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5440
 
HpaI GTT'AAC

Cuts at:   3030    3030 
   Size:       5440
 
KpnI G_GTAC'C

Cuts at:   2765    2765 
   Size:       5440
 
MfeI C'AATT_G

Cuts at:   3039    3039 
   Size:       5440
 
MluI A'CGCG_T

Cuts at:   5316    5316 
   Size:       5440
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2094    4554    4738    1305 
   Size:        789    2460     184    2007

  Fragments arranged by size: 

                2460    2007     789     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2609      10 
   Size:         54    1425     343     777    2841

  Fragments arranged by size: 

                2841    1425     777     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2321    2422    3398    3757    3916     518 
   Size:        648    1155     101     976     359     159    2042

  Fragments arranged by size: 

                2042    1155     976     648     359     159     101
 
MspA1I CmG'CkG

Cuts at:   1606    2115    2251    2856    3533    4681    4926    1606 
   Size:        509     136     605     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     605     509     245     136
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3949

  Fragments arranged by size: 

                3949     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5440
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5440
 
NotI GC'GGCC_GC

Cuts at:   2887    2887 
   Size:       5440
 
NspI r_CATG'y

Cuts at:   1132    1229    2798    4343    1132 
   Size:         97    1569    1545    2229

  Fragments arranged by size: 

                2229    1569    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5440
 
PciI A'CATG_T

Cuts at:   4339    4339 
   Size:       5440
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5440
 
PmeI GTTT'AAAC

Cuts at:   2744    2744 
   Size:       5440
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3509    3541    5022    5054    3509 
   Size:         32    1481      32    3895

  Fragments arranged by size: 

                3895    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2667    2667 
   Size:       5440
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5440
 
PsiI TTA'TAA

Cuts at:   3010    3010 
   Size:       5440
 
PstI C_TGCA'G

Cuts at:    830    2256    2578    2792     830 
   Size:       1426     322     214    3478

  Fragments arranged by size: 

                3478    1426     322     214
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5440
 
PvuII CAG'CTG

Cuts at:   2115    2251    2115 
   Size:        136    5304
 
SalI G'TCGA_C

Cuts at:   1936    2782    1936 
   Size:        846    4594
 
SanDI GG'GwC_CC

Cuts at:   2667    2667 
   Size:       5440
 
SbfI CC_TGCA'GG

Cuts at:   2792    2792 
   Size:       5440
 
ScaI AGT'ACT

Cuts at:   3688    5400    3688 
   Size:       1712    3728
 
SfcI C'TryA_G

Cuts at:    826    1045    2252    2574    2788    3923    4604    4795     826 
   Size:        219    1207     322     214    1135     681     191    1471

  Fragments arranged by size: 

                1471    1207    1135     681     322     219     214     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5440
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5440
 
SmaI CCC'GGG

Cuts at:   1872    2147    2767    1872 
   Size:        275     620    4545

  Fragments arranged by size: 

                4545     620     275
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2201    3544    4445    4707     820 
   Size:        197     926     258    1343     901     262    1553

  Fragments arranged by size: 

                1553    1343     926     901     262     258     197
 
SnaBI TAC'GTA

Cuts at:    493    3231     493 
   Size:       2738    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5440
 
SphI G_CATG'C

Cuts at:   1132    2798    1132 
   Size:       1666    3774
 
SspI AAT'ATT

Cuts at:      5      52    3364       5 
   Size:         47    3312    2081

  Fragments arranged by size: 

                3312    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5440
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2347    2879     513 
   Size:        552     939     343     532    3074

  Fragments arranged by size: 

                3074     939     552     532     343
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3467    3484    3637    3822    4267     325 
   Size:       1255    1887      17     153     185     445    1498

  Fragments arranged by size: 

                1887    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2367    2397    2613 
   Size:        274      80      33      51    1443     390      30     216

Cuts at:   2613    3686    5398      96 
   Size:       1073    1712     138

  Fragments arranged by size: 

                1712    1443    1073     390     274     216     138      80
                  51      33      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5400    5432    5400 
   Size:         32    5408
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5440
 
XbaI T'CTAG_A

Cuts at:   2776    2776 
   Size:       5440
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2368    2368 
   Size:       5440
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5440
 
XmaI C'CCGG_G

Cuts at:   1870    2145    2765    1870 
   Size:        275     620    4545

  Fragments arranged by size: 

                4545     620     275
 
XmnI GAAnn'nnTTC

Cuts at:   3569    3569 
   Size:       5440
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3248     276 
   Size:         53      83     186     936    1714    2468

  Fragments arranged by size: 

                2468    1714     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI
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