################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsmBI BsmI Bsp1407I BspDI BspHI BspMI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI HphI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 904 GGTACC G^GTACC AccIII 567 TCCGGA T^CCGGA AcyI 203 GGCGCC G[GA]^CG[CT]C AflIII 1028 ACATGT A^C[GA][CT]GT AlwNI 1051 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 871 AGCGCT AGC^GCT ApaI 755 GGGCCC GGGCC^C Asp718I 904 GGTACC G^GTACC AvaI 880 CCCGGG C^[CT]CG[GA]G BalI 1022 TGGCCA TGG^CCA BamHI 728 GGATCC G^GATCC BbeI 203 GGCGCC GGCGC^C BbvCI 412 GCTGAGG CCTCAGC(-5/-2) BfaI 703 CTAG C^TAG BglI 430 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 97 TACGTG [CT]AC^GT[GA] BsaHI 203 GGCGCC G[GA]^CG[CT]C BseAI 567 TCCGGA T^CCGGA BsiEI 338 CGATCG CG[GA][CT]^CG BsiWI 321 CGTACG C^GTACG BsmAI 549 GTCTC GTCTC(1/5) BsoBI 880 CCCGGG C^[CT]CG[GA]G BspEI 567 TCCGGA T^CCGGA BspLU11I 1028 ACATGT A^CATGT BsrDI 170 CATTGC GCAATG(2/0) BssHII 292 GCGCGC G^CGCGC BstXI 512 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 728 GGATCC [GA]^GATC[CT] DraIII 217 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 109 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 871 AGCGCT AGC^GCT EcoT22I 991 ATGCAT ATGCA^T Hin1I 203 GGCGCC G[GA]^CG[CT]C KasI 203 GGCGCC G^GCGCC KpnI 904 GGTACC GGTAC^C MaeI 703 CTAG C^TAG MflI 728 GGATCC [GA]^GATC[CT] MluNI 1022 TGGCCA TGG^CCA MroI 567 TCCGGA T^CCGGA MscI 1022 TGGCCA TGG^CCA MseI 242 TTAA T^TAA NaeI 44 GCCGGC GCC^GGC NarI 203 GGCGCC GG^CGCC NcoI 941 CCATGG C^CATGG NgoMIV 44 GCCGGC G^CCGGC NsiI 991 ATGCAT ATGCA^T NspI 1028 ACATGT [GA]CATG^[CT] PflMI 216 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 338 CGATCG CGAT^CG PvuII 113 CAGCTG CAG^CTG SapI 867 GAAGAGC GCTCTTC(1/4) ScaI 840 AGTACT AGT^ACT SexAI 824 ACCAGGT A^CC[AT]GGT SfaNI 993 GCATC GCATC(5/9) SfcI 479 CTGTAG C^T[GA][CT]AG SfoI 203 GGCGCC GGC^GCC SmaI 880 CCCGGG CCC^GGG Tru9I 242 TTAA T^TAA TspRI 390 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 216 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 728 GGATCC [GA]^GATC[CT] XmaCI 880 CCCGGG C^CCGGG XmaI 880 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 313 GTCTAC GT^[AC][GT]AC 774 GTCTAC AlwI 729 GATCC GGATC(4/5) 728 GGATC AocI 349 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG AspI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 823 GACCAGGTC BsaWI 567 TCCGGA [AT]^CCGG[AT] 598 TCCGGT BseRI 415 GAGGAG GAGGAG(10/8) 706 GAGGAG BsgI 811 CTGCAC GTGCAG(16/14) 1057 CTGCAC BsrFI 44 GCCGGC [GA]^CCGG[CT] 677 ACCGGC BssSI 831 CACGAG CACGAG(-5/-1) 7 CTCGTG BstF5I 228 CATCC GGATG(2/0) 994 CATCC Bsu36I 349 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG Cfr10I 44 GCCGGC [GA]^CCGG[CT] 677 ACCGGC DpnII 339 GATC ^GATC 729 GATC DsaI 194 CCGTGG C^C[GA][CT]GG 941 CCATGG EarI 55 GAAGAG CTCTTC(1/4) 867 GAAGAG Eco81I 349 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG EcoT14I 512 CCAAGG C^C[AT][AT]GG 941 CCATGG FokI 228 CATCC GGATG(9/13) 994 CATCC HgaI 109 GACGC GACGC(5/10) 449 GCGTC Ksp632I 55 GAAGAG CTCTTC(1/4) 867 GAAGAG MboI 339 GATC ^GATC 729 GATC MslI 690 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 984 CACTAAAATG NdeII 339 GATC ^GATC 729 GATC PflFI 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 823 GACCAGGTC PpuMI 131 AGGACCC [GA]G^G[AT]CC[CT] 345 GGGACCT SacI 179 GAGCTC GAGCT^C 268 GAGCTC Sau3AI 339 GATC ^GATC 729 GATC StyI 512 CCAAGG C^C[AT][AT]GG 941 CCATGG Tsp45I 562 GTGAC ^GT[GC]AC 829 GTCAC Tth111I 220 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 823 GACCAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI TfiI TseI